Table of Contents
7. Draining (AN7).
8. Dispensing rinsing solution (distilled water) (DN7).
9. Draining (AN4).
10. Dispensing methanol (DN4).
11. Slide removal.
Needle
DN1 (Undil. Stain 1)
DN2 (Buffer)
DN3 (Dil. Stain 2)
DN4 (Methanol)
DN7 (Rinse)
AN2
AN3
AN4
AN7
NOTE:
Methanol Volume (mL)
Stain Volume (mL)
Dilution Ratio (%)
Duration
Principle of the Procedure
The stained smear is examined microscopically; the nucleus and the cytoplasm of
neutrophils, lymphocytes, monocytes, eosinophils, and basophils show a
characteristic blue or red coloration. Cells are then manually differentiated and
quantified.
Cell Type
Neutrophils
Lymphocytes
Monocytes
Eosinophils
ADVIA Autoslide Slide Maker Stainer
Position
Needles AN2 and DN2 in position 22 are unused in this protocol.
4
3.6
25
Long
Cell Component
Nuclei
Cytoplasm
Granules
Nuclei
Cytoplasm
Nuclei
Cytoplasm
Granules
Nuclei
Action
32
1
22
0
8
1
31
0
24
2
22
0
8
1
31
1
24
2
Color
Vibrant deep purple
Pink background
Light purple-violet
Distinct deep purple
Sky blue to deep blue
Light purple with distinct spaces
between chromatin strands
Dull blue-gray with lightly stained
Fine pink to violet and evenly
distributed
Pale purplish-blue and well defined
10-33
Chapters
Table of Contents
Troubleshooting
