Table of Contents
Bibliography
Duck-Chong CG: Differential effect of detergents on the alkaline denaturation of
haemoglobin in maternal and fetal blood, with particular reference to Triton X-
100. J Clin Pathol 36:910 (1983)
Simplicio and Schwenzer, Biochem 12:1923 (1983)
Malin MJ, Sclafani LD and Wyatt JL: Evaluation of 24-second cyanide-
containing and cyanide-free methods for whole blood hemoglobin on the
Technicon H-1 analyzer with normal and abnormal blood samples. Am J Clin
Path 92:286 (1989)
Malin MJ, Fan SS and Benezra J: Mechanism of automated alkaline methods for
the determination of hemoglobin in whole blood based on the micellization of
ligated heme in the presence and absence of cyanide. Anal Chim Acta 262:67
(1992)
Measurement
Optical readings are obtained colorimetrically at 565 or 546 nm according to the
method installed.
After processing, the optical data are plotted on the hemoglobin rate curve where
time in seconds is plotted along the x axis, and the percent light transmission is
plotted along the y axis.
The hemoglobin transmission histogram is divided into five parts:
1. ADVIA 2 120/2120i SHEATH/RINSE reading from previous cycle
2. Draining of the ADVIA 2120/2120i SHEATH/RINSE, and refilling with the
reaction solution consisting of sample and ADVIA 2120/2120i HGB reagent
3. Reaction solution readings (15.5s to 18.0s) - Sample Mean
4. Draining of the reaction solution, and refilling with ADVIA 2120/2120i
SHEATH/RINSE
5. ADVIA 2120/2120i SHEATH/RINSE readings (baseline transmittance) for
the current sample − Baseline Mean value should be between 2.5 and 4.1.
8-22
HGB Trans
Methods
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Troubleshooting
