GE SEMIPHOR TRANSPHOR Series Operating Instructions Manual
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GE SEMIPHOR TRANSPHOR Series Operating Instructions Manual

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SEMIPHOR TRANSPHOR series
Operating Instructions
Original instructions
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  • Page 1 SEMIPHOR TRANSPHOR series Operating Instructions Original instructions...

  • Page 2: Table Of Contents

    Table of Contents Table of Contents Introduction ......................About this manual ..........................Important user information ......................Regulatory information ........................1.3.1 EU directives ............................. 1.3.2 Eurasian Customs Union ......................1.3.3 Regulations for USA and Canada ................... 1.3.4 Other regulations and standards .................... Safety instructions ....................

  • Page 3: Introduction

    1 Introduction Introduction About this chapter This chapter contains important user information, descriptions of safety notices, regula- tory information, and intended use of the SEMIPHOR TRANSPHOR UNIT series. In this chapter Section See page 1.1 About this manual 1.2 Important user information 1.3 Regulatory information SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA...

  • Page 4: About This Manual

    1 Introduction 1.1 About this manual About this manual Purpose of this manual The Operating Instructions provide you with the information needed to install, operate and maintain the product in a safe way. Scope of this manual The Operating Instructions cover the SEMIPHOR TRANSPHOR UNIT series, including SEMIPHOR TRANSPHOR UNIT, LARGE SEMIPHOR TRANSPHOR UNIT, PWR LARGE SEMIPHOR TRANSPHOR UNIT, and PWR SEMIPHOR TRANSPHOR UNIT.
  • Page 5 1 Introduction 1.1 About this manual Software items are identified in the text by bold italic text. A colon separates menu levels, thus File:Open refers to the Open command in the File menu. Hardware items are identified in the text by bold text (for example, Power). SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA...

  • Page 6: Important User Information

    1 Introduction 1.2 Important user information Important user information Read this before operating the product All users must read the entire Operating Instructions before installing, operating or maintaining the product. Always keep the Operating Instructions at hand when operating the product. Do not operate the product in any other way than described in the user documentation.
  • Page 7 1 Introduction 1.2 Important user information Safety notices This user documentation contains safety notices (WARNING, CAUTION, and NOTICE) concerning the safe use of the product. See definitions below. WARNING WARNING indicates a hazardous situation which, if not avoided, could result in death or serious injury. It is important not to proceed until all stated conditions are met and clearly understood.

  • Page 8: Regulatory Information

    UNIT series. Manufacturing information The table below summarizes the required manufacturing information. Requirement Information Name and address of manufacturer GE Healthcare Bio-Sciences AB, Björkgatan 30, SE 751 84 Uppsala, Sweden In this section Section See page 1.3.1 EU directives 1.3.2 Eurasian Customs Union 1.3.3 Regulations for USA and Canada...

  • Page 9: Eu Directives

    Operating Instructions or user manuals, and • used in the same state as it was delivered from GE Healthcare, except for alterations described in the Operating Instructions or user manuals. SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA...

  • Page 10: Eurasian Customs Union

    Telephone: + 46 771 400 600 Importer and/or company for obtain- GE Healthcare LLC ing information about importer GE Healthcare Life Sciences Presnenskaya nab., 10C, 12th floor RU-123 317 Moscow, Russian Federation Telephone 1: + 7 495 411 9714 Fax nr: + 7 495 739 6932 Email: [email protected]...

  • Page 11: Regulations For Usa And Canada

    The user is cautioned that any changes or modifications not expressly approved by GE Healthcare could void the user’s authority to operate the equipment. This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to part 15 of the FCC Rules.
  • Page 12 1 Introduction 1.3 Regulatory information 1.3.3 Regulations for USA and Canada • Consult the dealer or an experienced radio/TV technician for help. SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA...

  • Page 13: Other Regulations And Standards

    1 Introduction 1.3 Regulatory information 1.3.4 Other regulations and standards 1.3.4 Other regulations and standards Introduction This section describes the standards that apply to the SEMIPHOR TRANSPHOR UNIT series. Environmental conformity This product conforms to the following environmental requirements. Requirement Title 2012/19/EU Waste Electrical and Electronic Equipment (WEEE) Directive...

  • Page 14: Safety Instructions

    2 Safety instructions Safety instructions About this chapter This chapter describes safety precautions, labels and symbols that are attached to the equipment. In addition, the chapter describes emergency and recovery procedures, and provides recycling information. Important WARNING Before installing, operating or maintaining the product, all users must read and understand the entire contents of this chapter to become aware of the hazards involved.

  • Page 15: Safety Precautions

    2 Safety instructions 2.1 Safety precautions Safety precautions Introduction SEMIPHOR TRANSPHOR UNIT series handle materials that are considered hazardous material and LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT are powered by an external power supply. Before installing, operating or maintaining the system, you must be aware of the hazards described in this manual.
  • Page 16 SEMIPHOR TRANSPHOR UNIT series Op- erating Instructions. WARNING Use only approved parts. Only spare parts and accessories that are approved or supplied by GE Healthcare may be used for maintaining or servicing the product. WARNING This instrument is designed for indoor laboratory use only.
  • Page 17 2 Safety instructions 2.1 Safety precautions WARNING Do not immerse the unit in water. CAUTION Never autoclave any component of the electrophoresis unit or casting kit. Personal protection WARNING Always use appropriate Personal Protective Equipment (PPE) during operation and maintenance of this product. WARNING Hazardous substances and biological agents.
  • Page 18 Power supply WARNING Power cord. Only use power cords with approved plugs delivered or approved by GE Healthcare. System operation WARNING The safety lid must be in place before connecting the power leads to a power supply.
  • Page 19 2 Safety instructions 2.1 Safety precautions WARNING The electric components in the transfer unit base must not become wet. WARNING Supply voltage. Before connecting the power cord, make sure that the supply voltage at the wall outlet corresponds to the marking on the instrument.
  • Page 20 2 Safety instructions 2.1 Safety precautions WARNING Decommissioning. Decontaminate the equipment before decom- missioning to make sure that hazardous residues are removed. SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA...

  • Page 21: Labels

    2 Safety instructions 2.2 Labels Labels Introduction This section describes the system label and other safety or regulatory labels that are attached to the product. Description of symbols on the system label The table below describes the various symbols that may be found on the system label Label Meaning Warning! Read the user documentation before using the system.
  • Page 22 2 Safety instructions 2.2 Labels Label Meaning Eurasian Conformity mark: the single conformity mark indicates that the product is approved for circulation on the markets of the member states of the Eurasian Customs Union. This symbol indicates that the product has been certified by Intertek, which is a US Occupational Safety and Health Administration Na- tionally Recognized Testing Laboratory (NRTL).

  • Page 23: Emergency Procedures

    2 Safety instructions 2.3 Emergency procedures Emergency procedures Introduction This section describes how to shut down SEMIPHOR TRANSPHOR UNIT series in an emergency situation, and the procedure for restarting SEMIPHOR TRANSPHOR UNIT series. The section also describes the result in the event of power failure. Precautions WARNING Make sure that there is access to the instrument power supply cord...
  • Page 24 2 Safety instructions 2.3 Emergency procedures Power failure In case of power failure to the SEMIPHOR TRANSPHOR UNIT series, the run is interrupted immediately. Restart LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT after emergency shutdown or power failure To restart the run after an emergency shutdown or power failure, follow these steps: Step Action Make sure all connections are in place.

  • Page 25: Recycling Information

    2 Safety instructions 2.4 Recycling information Recycling information Introduction This section contains information about the decommissioning of the product. Decontamination The product must be decontaminated before decommissioning. All local regulations must be followed with regard to scrapping of the equipment. Disposal of the product When taking the product out of service, the different materials must be separated and recycled according to national and local environmental regulations.

  • Page 26: Declaration Of Hazardous Substances (Dohs)

    2 Safety instructions 2.5 Declaration of Hazardous Substances (DoHS) Declaration of Hazardous Substances (DoHS) 根据SJ/T11364-2014《电子电气产品有害物质限制使用标识要求》特提供如下有关污染控制方面的 信息。 The following product pollution control information is provided according to SJ/T11364-2014 Marking for Restriction of Hazardous Substances caused by electrical and electronic products. 电子信息产品污染控制标志说明 Explanation of Pollution Control Label 该标志表明本产品不含有超过中国标准...
  • Page 27 2 Safety instructions 2.5 Declaration of Hazardous Substances (DoHS) 有害物质的名称及含量 Name and Concentration of Hazardous Substances 产品中有害物质的名称及含量 Table of Hazardous Substances’ Name and Concentration 部件名称 有害物质 Component name Hazardous substance 铅 汞 镉 六价铬 多溴联苯 多溴二苯醚 (Pb) (Hg) (Cd) (Cr(VI)) (PBB) (PBDE) 11001342...

  • Page 28: System Description

    3 System description System description About this chapter This chapter gives an overview of the SEMIPHOR TRANSPHOR UNIT series, and a brief description of its function. In this chapter Section See page 3.1 Instrument overview 3.2 Detailed description SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA...

  • Page 29: Instrument Overview

    3 System description 3.1 Instrument overview Instrument overview Introduction to SEMIPHOR TRANSPHOR UNIT series The SEMIPHOR TRANSPHOR UNIT series are for preparative blotting of proteins. PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT have a built-in power supply and are operated and controlled from the instrument keypad. This section gives an overview of the SEMIPHOR TRANSPHOR UNIT series.
  • Page 30 3 System description 3.1 Instrument overview Illustration of the PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT The illustration below shows an example of PWR LARGE SEMIPHOR TRANSPHOR UNIT or PWR SEMIPHOR TRANSPHOR UNIT. Difference SEMIPHOR TRANSPHOR UNIT series The SEMIPHOR TRANSPHOR UNIT series differ by their transfer surface.

  • Page 31: Detailed Description

    3 System description 3.2 Detailed description Detailed description Introduction This section gives an overview of the SEMIPHOR TRANSPHOR UNIT series. Illustration of LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT The illustration below shows and gives a brief description of the LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT.
  • Page 32 3 System description 3.2 Detailed description Illustration of PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT The illustration below shows the parts and gives a brief description of the PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT. Part Function Power cord...
  • Page 33 3 System description 3.2 Detailed description Illustration of the PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT keypad The illustration below shows the parts and gives a brief description of the keypad of PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT, placed on the top panel of the instrument.

  • Page 34: Installation

    Inspect all visible parts for damage or missing pieces. If any damage is observed, record this on the receiving documents and inform your GE Healthcare representative. Make sure to keep all packing material for damage claims or to use should it become necessary to return the unit.

  • Page 35: Operation

    The electric components in the transfer unit base must not become wet. WARNING Do not immerse the unit in water. WARNING Power cord. Only use power cords with approved plugs delivered or approved by GE Healthcare. SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA...
  • Page 36 WARNING Power cord. Only use power cords with approved plugs delivered or approved by GE Healthcare. In this chapter Section See page 5.1 Assemble transfer stack 5.2 Electrotransfer with LARGE SEMIPHOR TRANSPHOR UNIT and...

  • Page 37: Assemble Transfer Stack

    5 Operation 5.1 Assemble transfer stack Assemble transfer stack Introduction A single or multiple gels can be transferred at the same time. Place multiple small gels of the same thickness side by side, or stack two gels vertically in a carefully constructed multi-layered stack.
  • Page 38 5 Operation 5.1 Assemble transfer stack Part Function Optional: Mylar™ mask (opening approximately 2 mm smaller than the gel on all sides) Preparations Follow the instructions below to prepare for making a transfer stack. Step Action Rinse the anode and cathode with distilled water. Cut away the wells and/or stacking gel section from the gel.
  • Page 39 5 Operation 5.1 Assemble transfer stack Step Action For each gel, cut at least 6 pieces of blotting paper the same size as the gel or slightly smaller. Make sure to cut the blotting paper to the same size as the gel.
  • Page 40 5 Operation 5.1 Assemble transfer stack Step Action Remove all trapped air by rolling a clean pipet or roller from the center toward the edges. Place the pre-wet membrane onto the stack of blotting paper. Place the gel on the membrane. NOTICE Take care to place the gel correctly on the first try be- cause proteins begin to transfer immediately;...
  • Page 41 5 Operation 5.1 Assemble transfer stack Part Function Blotting paper (2 to 3 sheets) Membrane Blotting paper (2 to 3 sheets) Optional: Mylar mask (opening approximately 2 mm smaller than the gels on all sides) The illustration below shows a vertical transfer stack. SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA...
  • Page 42 5 Operation 5.1 Assemble transfer stack Part Function Blotting paper (2 to 3 sheets) Membrane Blotting paper (1 sheet) Cellophane Membrane Blotting paper (2 to 3 sheets) SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA...
  • Page 43 5 Operation 5.1 Assemble transfer stack Part Function Optional: Mylar mask (opening approximately 2 mm smaller than the gels on all sides) Assemble parallel transfer stacks To assemble a transfer stack with multiple gels, either place gels of the same thickness side-by-side to create parallel stacks or place the gels in a vertical stack.
  • Page 44 5 Operation 5.1 Assemble transfer stack Assemble a transfer stack with multiple gels Follow the instructions below to assemble a vertical transfer stack. Tip: When transferring multiple gels, transfer efficiency depends on factors such as gel thickness, gel position in the stack, transfer buffer, membrane type, and, most importantly, the characteristics of the protein.

  • Page 45: Electrotransfer With Large Semiphor Transphor Unit And Semiphor Transphor Unit

    5 Operation 5.2 Electrotransfer with LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT Electrotransfer with LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT Introduction Electrotransfer with LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT are performed at low current and low voltage, in approximately one hour. This section describes how to run the electrotransfer using LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT.
  • Page 46 5 Operation 5.2 Electrotransfer with LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT Electrotransfer with LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT Follow the instructions below to start an electrotransfer with the LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT. Step Action Make sure that the power supply is switched OFF.

  • Page 47: Electrotransfer With Pwr Large Semiphor Transphor Unit And Pwr Semiphor Transphor Unit

    5 Operation 5.3 Electrotransfer with PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT Electrotransfer with PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT Introduction Electrotransfer with PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT are performed at low current and low voltage, in approximately one hour.
  • Page 48 5 Operation 5.3 Electrotransfer with PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT Electrotransfer with PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT Follow the instructions below to start an electrotransfer with PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT. Step Action Turn the instrument on using the POWER button on the front keypad.
  • Page 49 5 Operation 5.3 Electrotransfer with PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT Step Action Press the START/STOP button to start the transfer. Tip: During the transfer, the voltage can be displayed. Result: A red LED will appear in the lower right corner of the display when voltage is applied to the transfer stack, as shown in the illustration below.

  • Page 50: Changing Parameters During The Transfer

    5 Operation 5.4 Changing parameters during the transfer Changing parameters during the transfer Introduction This section describes how to change parameters or add buffer during the electrotransfer using PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT. Changing the time or current Both the time and the current can be changed during the electrotransfer run.
  • Page 51 5 Operation 5.4 Changing parameters during the transfer Buffer depletion One of the more common problems for a semi-dry electrotransfer is buffer depletion. Buffer depletion leads to changes in pH and overheating, both of which are detrimental to the transfer. PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT monitor the transfer stack resistance.

  • Page 52: After The Transfer Is Complete

    5 Operation 5.5 After the transfer is complete After the transfer is complete Introduction This section describes how to remove the gel from the transfer stack and how to turn off PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT. After a transfer with LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT...
  • Page 53 5 Operation 5.5 After the transfer is complete Step Action If desired, label the gel-contact-side of the membrane with a soft pencil. Remove the membrane(s) from the stack with blunt forceps. Process the membrane according to your protocol or allow the membrane to air dry prior to storage.

  • Page 54: Maintenance

    WARNING Use only approved parts. Only spare parts and accessories that are approved or supplied by GE Healthcare may be used for maintaining or servicing the product. WARNING Disconnect power. Always disconnect power from the instrument before performing any maintenance task.
  • Page 55 6 Maintenance NOTICE Cleaning. Keep the exterior of the instrument dry and clean. Wipe regularly with a soft damp tissue and, if necessary, a mild cleaning agent. Let the instrument dry completely before use. General cleaning procedure When cleaning the SEMIPHOR TRANSPHOR UNIT series make sure to: •...

  • Page 56: Troubleshooting

    If the suggested actions in this guide do not solve the problem, or if the problem is not covered by this guide, contact your GE Healthcare representative for advice. Safety precautions...
  • Page 57 7 Troubleshooting Problem Solution Molecules do not migrate out of Check all electrical connections. Confirm that current is flowing through the transfer stack. Increase transfer period. Large fragments may require an additional hour. Do not use staining or fixing agents on the gel before transfer.
  • Page 58 7 Troubleshooting Open circuit or no output current for LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT Problem Solution Open circuit or no output cur- Push circuit protection reset button located next rent for LARGE SEMIPHOR to the input leads. To prevent recurrence limit the TRANSPHOR UNIT and input voltage to 30 V and the current to 500 mA.
  • Page 59 7 Troubleshooting Uneven band transfer Problem Solution Uneven band transfer The blotting paper and membrane must be the same size as the gel or 1 to 2 mm smaller. Larger sizes will provide an electrical path for current to bypass the gel solution. Different proteins will transfer at different rates depending on size and net charge.
  • Page 60 7 Troubleshooting PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT Problem Solution Current reading lower than set- Maximum voltage (30 V) has been reached. Buffer ting may be depleted. No output current Transfer stack is not making contact with the up- per electrode.

  • Page 61: Reference Information

    8 Reference information Reference information About this chapter This chapter lists the technical specifications of SEMIPHOR TRANSPHOR UNIT series. The chapter also includes ordering information and Health and Safety Declaration form for service. In this chapter Section See page 8.1 Specifications 8.2 Ordering information 8.3 Health and Safety Declaration Forms SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA...

  • Page 62: Specifications

    8 Reference information 8.1 Specifications Specifications LARGE SEMIPHOR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR UNIT specification Characteristic Description SEMIPHOR LARGE SEMIPHOR TRANSPHOR UNIT TRANSPHOR UNIT Electrode size (W × H) 14 × 16 cm 21 × 26 cm Inputs: Voltage (VDC) Current (A) Output: Voltage (VDC)
  • Page 63 8 Reference information 8.1 Specifications PWR LARGE SEMIPHOR TRANSPHOR UNIT and PWR SEMIPHOR TRANSPHOR UNIT specification Characteristic Description PWR SEMIPHOR PWR LARGE SEMIPHOR TRANSPHOR UNIT TRANSPHOR UNIT Electrode size (W × H) 14 × 16 cm 21 × 26 cm Inputs: Voltage (VDC) Current (A)

  • Page 64: Ordering Information

    8 Reference information 8.2 Ordering information Ordering information For product codes and information about how to order, please see www.gelifesciences.com SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA...

  • Page 65: Health And Safety Declaration Forms

    Service Ticket #: To make the mutual protection and safety of GE service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, please complete this checklist and present it to the Service Engineer upon arrival.
  • Page 66 To make sure the mutual protection and safety of GE personnel, our customers, transportation personnel and our environment, all equipment must be clean and free of any hazardous contaminants before shipping to GE. To avoid delays in the processing of your equipment, please complete this checklist and include it with your return.

  • Page 67: Index

    Index Index Assemble Important user information, 6 multiple transfer stack, 43 Incomplete transfer, 56 single transfer stack, 38–39 Intended use, 6 Labels, 21 conformity, 9 Safety labels, 22 marking, 9 LARGE SEMIPHOR TRANSPHOR Cleaning, 55 UNIT and SEMIPHOR Before service, 55 TRANSPHOR UNIT Emergency shutdown, 23 instrument modules, descrip-...
  • Page 68 Index PWR LARGE SEMIPHOR LARGE SEMIPHOR TRANSPHOR UNIT and PWR TRANSPHOR UNIT and SEMIPHOR TRANSPHOR SEMIPHOR TRANSPHOR UNIT, 63 UNIT, 45, 47 Purpose of this manual, 4 Service PWR LARGE SEMIPHOR Cleaning before, 55 TRANSPHOR UNIT and PWR Single transfer stack, 37 SEMIPHOR TRANSPHOR UNIT Standards, 13 change current, 50...
  • Page 69 Amersham Place All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms Little Chalfont and conditions is available on request. Contact your local GE Healthcare repre- sentative for the most current information.

This manual is also suitable for:

Pwr semiphor transphor unitSemiphor transphor unitPwr large semiphor transphor unitLarge semiphor transphor unit

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