Table of Contents
Table of Contents
Related Manuals for GE SE 250 Mighty Small II
Summary of Contents for GE SE 250 Mighty Small II
- Page 1 SE 250/260 Mighty Small II Operating Instructions Original instructions...
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Page 2: Table Of Contents
Table of Contents Table of Contents Introduction ......................About this manual ..........................Important user information ......................Regulatory information ........................1.3.1 EU directives ............................. 1.3.2 Eurasian Customs Union ......................1.3.3 Regulations for USA and Canada ................... 1.3.4 Other regulations and standards .................... Safety information .................... -
Page 3: Introduction
1 Introduction Introduction About this chapter This chapter contains important user information, descriptions of safety notices, regula- tory information, and intended use of the SE 250/260 Mighty Small II. In this chapter Section See page 1.1 About this manual 1.2 Important user information 1.3 Regulatory information SE 250/260 Mighty Small II Operating Instructions 29281629 AA... -
Page 4: About This Manual
1 Introduction 1.1 About this manual About this manual Purpose of this manual The Operating instructions provide you with the information needed to install, operate and maintain the product in a safe way. Scope of this manual The Operating Instructions covers the SE 250/260 Mighty Small II. The illustration below shows the SE 250/260 Mighty Small II. -
Page 5: Important User Information
1 Introduction 1.2 Important user information Important user information Read this before operating the product All users must read the entire Operating instruction before installing, operating or maintaining the product. Always keep the Operating instruction at hand when operating the product. Do not operate the product in any other way than described in the user documentation. - Page 6 1 Introduction 1.2 Important user information Safety notices This user documentation contains safety notices (WARNING, CAUTION, and NOTICE) concerning the safe use of the product. See definitions below. WARNING WARNING indicates a hazardous situation which, if not avoided, could result in death or serious injury. It is important not to proceed until all stated conditions are met and clearly understood.
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Page 7: Regulatory Information
Small II. Manufacturing information The table below summarizes the required manufacturing information. Requirement Information Name and address of manufacturer GE Healthcare Bio-Sciences AB, Björkgatan 30, SE 751 84 Uppsala, Sweden In this section Section See page 1.3.1 EU directives 1.3.2 Eurasian Customs Union 1.3.3 Regulations for USA and Canada... -
Page 8: Eu Directives
Operating Instructions or user manuals, and • used in the same state as it was delivered from GE, except for alterations described in the Operating Instructions or user manuals. SE 250/260 Mighty Small II Operating Instructions 29281629 AA... -
Page 9: Eurasian Customs Union
Telephone: + 46 771 400 600 Importer and/or company for obtain- GE Healthcare LLC ing information about importer GE Healthcare Life Sciences Presnenskaya nab., 10C, 12th floor RU-123 317 Moscow, Russian Federation Telephone 1: + 7 495 411 9714 Fax nr: + 7 495 739 6932 Email: [email protected]... -
Page 10: Regulations For Usa And Canada
1 Introduction 1.3 Regulatory information 1.3.3 Regulations for USA and Canada 1.3.3 Regulations for USA and Canada Introduction This section describes the regulations that apply to SE 250/260 Mighty Small II in the USA and Canada. NRTL certification This symbol indicates that the product has been certified by Intertek, which is a US Oc- cupational Safety and Health Administration Nationally Recognized Testing Laboratory (NRTL). -
Page 11: Other Regulations And Standards
1 Introduction 1.3 Regulatory information 1.3.4 Other regulations and standards 1.3.4 Other regulations and standards Introduction This section describes the standards that apply to the SE 250/260 Mighty Small II. Environmental conformity This product conforms to the following environmental requirements. Requirement Title 2012/19/EU... -
Page 12: Safety Information
2 Safety information Safety information About this chapter This chapter describes safety precautions, labels and symbols that are attached to the equipment. In addition, the chapter describes emergency and recovery procedures, and provides recycling information. Important WARNING Before installing, operating or maintaining the product, all users must read and understand the entire contents of this chapter to become aware of the hazards involved. -
Page 13: Safety Precautions
2 Safety information 2.1 Safety precautions Safety precautions Introduction The SE 250/260 Mighty Small II is powered by an external power supply. Before installing, operating or maintaining the system, you must be aware of the hazards described in this manual. Follow the instructions provided to avoid injury to the operator or other personnel, to the product, or to other equipment in the area. - Page 14 Do not damage the power supply cord by bending, twisting, heating or allowing them to become pinned under the equipment. Using damaged power cords could result in fire or electric shock. If the power supply cords are damaged, contact your local GE representative for replacements. WARNING The safety lid must be in place before connecting the power leads to a power supply.
- Page 15 2 Safety information 2.1 Safety precautions Personal protection WARNING Always use appropriate Personal Protective Equipment (PPE) during operation and maintenance of this product. WARNING Hazardous substances and biological agents. When using haz- ardous chemical and biological agents, take all suitable protective measures, such as wearing protective clothing, glasses and gloves resistant to the substances used.
- Page 16 Handle the unit with care to avoid personal injury. Power supply WARNING Power cord. Only use power cords with approved plugs delivered or approved by GE. WARNING The safety lid must be in place before connecting the power leads to a power supply.
- Page 17 2 Safety information 2.1 Safety precautions WARNING Make sure that there is access to the instrument power supply cord at all times. Operation WARNING The high voltage power supply must always be disconnected when the safety lid of the electrophoresis unit is taken off. The high volt- age power supply must never be switched on unless the safety lid is on the electrophoresis unit.
- Page 18 2 Safety information 2.1 Safety precautions CAUTION Circulate coolant through the heat exchanger to minimize heating. Overheating will cause irreparable damage to the unit! Do not connect the heat exchanger to a water tap or any coolant source where the water pressure is unregulated. CAUTION Do not operate with buffer temperatures above the maximum specified technical specifications.
- Page 19 2 Safety information 2.1 Safety precautions WARNING Use only approved parts. Only spare parts and accessories that are approved or supplied by GE may be used for maintaining or servicing the product. WARNING Disconnect power. Always disconnect power from the instrument before performing any maintenance task.
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Page 20: Labels
2 Safety information 2.2 Labels Labels Introduction This section describes the system label and other safety or regulatory labels that are attached to the product. Description of symbols on the system label The table below describes the various symbols that may be found on the system label. Label Meaning Warning! Read the user documentation before using the system. - Page 21 2 Safety information 2.2 Labels Label Meaning This symbol indicates that the product has been certified by Intertek, which is a US Occupational Safety and Health Administration Na- tionally Recognized Testing Laboratory (NRTL). Serial no.: Serial number of the product Manufac- Year (YYYY) and month (MM) of manufacture tured:...
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Page 22: Emergency Procedures
2 Safety information 2.3 Emergency procedures Emergency procedures Introduction This section describes how to shut down of the SE 250/260 Mighty Small II in an emer- gency situation, and the procedure for restarting the SE 250/260 Mighty Small II. The section also describes the result in the event of power failure. Precautions WARNING Make sure that there is access to the instrument power supply cord... -
Page 23: Recycling Information
2 Safety information 2.4 Recycling information Recycling information Introduction This section contains information about the decommissioning of the product. Decontamination The product must be decontaminated before decommissioning. All local regulations must be followed with regard to scrapping of the equipment. Disposal of the product When taking the product out of service, the different materials must be separated and recycled according to national and local environmental regulations. -
Page 24: Declaration Of Hazardous Substances (Dohs)
2 Safety information 2.5 Declaration of Hazardous Substances (DoHS) Declaration of Hazardous Substances (DoHS) 根据SJ/T11364-2014《电子电气产品有害物质限制使用标识要求》特提供如下有关污染控制方面的 信息。 The following product pollution control information is provided according to SJ/T11364-2014 Marking for Restriction of Hazardous Substances caused by electrical and electronic products. 电子信息产品污染控制标志说明 Explanation of Pollution Control Label 该标志表明本产品不含有超过中国标准... - Page 25 2 Safety information 2.5 Declaration of Hazardous Substances (DoHS) 有害物质的名称及含量 Name and Concentration of Hazardous Substances 产品中有害物质的名称及含量 Table of Hazardous Substances’ Name and Concentration 部件名称 有害物质 Component name Hazardous substance 铅 汞 镉 六价铬 多溴联苯 多溴二苯醚 (Pb) (Hg) (Cd) (Cr(VI)) (PBB) (PBDE) 80614745...
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Page 26: System Description
3 System description System description About this chapter This chapter gives an overview of the SE 250/260 Mighty Small II. Illustration of the instrument The illustration below shows the SE 250/260 Mighty Small II instrument. Part Function Color-coded leads (2) SE 250/260 Mighty Small II Operating Instructions 29281629 AA... - Page 27 3 System description Part Function Safety lid Upper buffer chamber core Foam gasket Coolant port (2) Spring clamps (4) Positioning tabs SE 250/260 Mighty Small II Lower buffer chamber The following parts are included, but not shown in the illustration: •...
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Page 28: Installation
Inspect all visible parts for damage or missing pieces. If any damage is observed, record this on the receiving documents and inform your GE representative. Make sure to keep all packing material for damage claims or to use should it become necessary to return the unit. -
Page 29: Operation
5 Operation Operation About this chapter This chapter gives instructions on how to operate the product in a safe way. Safety precautions WARNING The high voltage power supply must always be disconnected when the safety lid of the electrophoresis unit is taken off. The high volt- age power supply must never be switched on unless the safety lid is on the electrophoresis unit. - Page 30 5 Operation CAUTION Circulate only water or 50/50 water/ethylene glycol through the heat exchanger. Never use anti-freeze or any organic solvent in the heat exchanger. CAUTION Devices must only be operated with a certified power supply capa- ble of limiting the voltage and current to specified ratings. NOTICE After initial monitoring, do not leave the unit unattended for more than 45 min before checking the progress of the bands and the...
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Page 31: Preparations
5 Operation 5.1 Preparations Preparations Prepare the gel sandwich Both precast and self-cast gels can be run in the SE 250/260 Mighty Small II units. This unit accepts gels in 10 × 8 cm plates, which can be cast in SE215, SE245, or SE275 gel casters. - Page 32 5 Operation 5.1 Preparations Optional cooling Circulating pressure must not exceed 0.8 bar (12 psi) above ambient pressure. Do not connect the cooling core to an unregulated coolant source such as a water tap. To connect the cooling core to a circulator bath, follow these steps: Step Action Slide hose clamps (4 in total) onto each end of two lengths of 8 mm (5/16")
- Page 33 5 Operation 5.1 Preparations Step Action To remove the core, depress both release tabs and lift. Part Description Handle Release tabs (2) Coolant port (2) SE 250/260 Mighty Small II Operating Instructions 29281629 AA...
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Page 34: Assembly
5 Operation 5.2 Assembly Assembly Place the gel sandwich in the SE 250/260 Mighty Small II WARNING Acrylamide is a neurotoxin. Always wear gloves and observe all laboratory safety procedures. Step Action Rinse away the gel overlay with distilled water and drain any excess water. If installing a self-cast or precast 10 ×... - Page 35 5 Operation 5.2 Assembly Step Action Rinse away the overlay with distilled water and drain any excess water. If installing a self-cast or precast 10 × 8 cm gel sandwich, align the bottom of the plate with the bottom of the core (see figure below). The bottom of the notched plate must cover the silicone rubber gasket.
- Page 36 5 Operation 5.2 Assembly Clamp the sandwich in place Note: Cooling is optional. If desired, attach tubing to ports on both sides of the core before attaching gel sandwiches. Circulate coolant. See Optional cooling, on page Step Action Lightly press the sandwich against the gasket and secure it to the core with one spring clamp on each side.
- Page 37 5 Operation 5.2 Assembly To prepare the wells, follow these steps: Step Action De-aerate the stacking gel monomer solution. Add catalyst and initiator to the stacking gel monomer solution and then pour. Use a pipette to deliver the solution into one corner of the plate, taking care not to trap any bubbles.
- Page 38 5 Operation 5.2 Assembly Step Action To aid in loading samples, wet the well-locating decal and apply it to the front of the glass plate so that the appropriate edge outlines the sample wells. Note: The side wells for standards of a preparative comb correspond to the outer- most wells formed by the 10-well comb.
- Page 39 5 Operation 5.2 Assembly Step Action Fill the lower buffer chamber with running buffer. The SE 250/260 Mighty Small II holds about 250 mL. Make sure that the lower electrode (running along the bottom of the the upper buffer chamber core) is completely submerged.
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Page 40: Electrophoresis Run
5 Operation 5.3 Electrophoresis run Electrophoresis run Running the gel Gels may be run at either constant current or constant voltage. A constant current setting is traditionally used with a discontinuous buffer system so that the rate of electrophoretic migration remains unchanged throughout the run. Under these conditions, voltage in- creases as the run proceeds. - Page 41 5 Operation 5.3 Electrophoresis run Step Action Remove the core assembly with gels attached by squeezing the release tabs and lift out the core assembly. Pour out the buffer by inverting the core assembly, then remove both clamps, and lift away gel sandwich(es) from the upper buffer chamber core. Gently loosen and then slide away both spacers.
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Page 42: Maintenance
WARNING Use only approved parts. Only spare parts and accessories that are approved or supplied by GE may be used for maintaining or servicing the product. WARNING Disconnect power. Always disconnect power from the instrument before performing any maintenance task. - Page 43 6 Maintenance Cleaning before planned maintenance/service To ensure the protection and safety of service personnel, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts maintenance work. Please complete the checklist in the On Site Service Health and Safety Declaration Form or the Health and Safety Declaration Form for Product Return or Servicing, depending on whether the instrument is going to be serviced on site or returned for service, respectively.
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Page 44: Troubleshooting
If the suggested actions in this guide do not solve the problem, or if the problem is not covered by this guide, contact your GE representative for advice. Safety precautions CAUTION Circulate only water or 50/50 water/ethylene glycol through the heat exchanger. - Page 45 7 Troubleshooting Error description Corrective action Protein streaks verti- Centrifuge or filter sample before loading to remove particu- cally lates. Dialyze or desalt the sample. Unusually slow (or To increase or decrease the migration rate, adjust the voltage fast) run or current by 25% to 50%.
- Page 46 7 Troubleshooting Error description Corrective action Poor band resolu- Use only the highest quality reagents. tion Conduct the separation at a lower current or voltage setting. Dialyze or desalt the sample. Reduce the sample volume or concentration. Only use freshly deionized urea. Improve dissociation of subunits by heating sample in SDS sample buffer 1–2 minutes at 100°C.
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Page 47: Reference Information
8 Reference information Reference information About this chapter This chapter lists the technical specifications of the SE 250/260 Mighty Small II. The chapter also includes ordering information, and the Health and Safety Declaration form for service. In this chapter Section See page 8.1 Specifications 8.2 Ordering information... -
Page 48: Specifications
8 Reference information 8.1 Specifications Specifications Parameter Specification Gel plate size 10 × 8 cm Approximate gel size 8 × 7 cm Maximum power 12 W Maximum voltage 500 V Maximum current 500 mA Maximum gel temperature 45°C Environmental operating Indoor use: 4°C to 40°C conditions Humidity up to 80%... -
Page 49: Ordering Information
8 Reference information 8.2 Ordering information Ordering information For product codes and information about how to order, please see www.gelifesciences.com SE 250/260 Mighty Small II Operating Instructions 29281629 AA... -
Page 50: Health And Safety Declaration Forms
Service Ticket #: To make the mutual protection and safety of GE service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, please complete this checklist and present it to the Service Engineer upon arrival. - Page 51 To make sure the mutual protection and safety of GE personnel, our customers, transportation personnel and our environment, all equipment must be clean and free of any hazardous contaminants before shipping to GE. To avoid delays in the processing of your equipment, please complete this checklist and include it with your return.
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Page 52: Index
Index Index Operation, 29 Ordering information, 49 conformity, 8 marking, 8 Prerequisites, 5 Cleaning, 42 Product return or servicing, 51 Before service, 43 Purpose of this manual, 4 Decommisioning, 23 Recycling information decontamination, 23 disposal of electrical compo- Emergency nents, 23 procedures, 22 Regulatory information, 7, 10 Emergency procedures... - Page 53 All goods and services are sold subject to the terms and conditions of sale of GE Healthcare UK Limited the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare repre- Amersham Place sentative for the most current information.