GE ImageQuant LAS 4000 User Manual
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GE ImageQuant LAS 4000 User Manual

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ImageQuant™ LAS 4000
User Manual
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Summary of Contents for GE ImageQuant LAS 4000

  • Page 1 GE Healthcare ImageQuant™ LAS 4000 User Manual...

  • Page 3: Table Of Contents

    Changing the UV transilluminator or white light table ..................3.3.2 Changing the Epi lights ..............................Operating the ImageQuant LAS 4000 ......................Layout of the ImageQuant LAS 4000 Control Software ..................Preparation for exposure ..............................Calibration ....................................Placing the sample ................................
  • Page 4 5.13 Help menu ....................................Installing ImageQuant LAS 4000 Control Software ................... Installation sequence ................................ Install ImageQuant LAS 4000 Control Software under Windows XP ............Install ImageQuant LAS 4000 Control Software under Windows Vista ............. Uninstalling and upgrading ImageQuant LAS 4000 Control Software ..........

  • Page 5: Introduction

    1 Introduction The ImageQuant LAS 4000 The ImageQuant LAS 4000 is a camera system for producing digital images of gel or membrane samples or films. It is used together with the ImageQuant LAS 4000 Control Software to: • Expose chemiluminescent samples or membranes.
  • Page 6 The ImageQuant LAS 4000 is a camera system that produces digital images of chemiluminescent, dyed or fluorescent gels and membranes. The ImageQuant LAS 4000 is intended for research use only, and shall not be used in any clinical procedures, or for diagnostic purposes.
  • Page 7 Do not use cables other than the power cable that comes with the equipment. 2 In EU countries: The cable is given the following CEE Certification Number. 6522-1570-8035/AIG DEMKO 116773 ASC SEMKO 9449029 KEMA 93.6830.04-KCS/LB NEMKO P94102620 CEBEC 8652 EI (FIMKO) 179615-02 0204-515-00 ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 8 Notes and tips Note: A Note is used to indicate information that is important for trouble-free and optimal use of the product. A tip contains useful information that can improve or optimize your procedures. TIP: ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 9 Software items are identified in the text by bold italic text. A colon separates menu levels, thus File:Open refers to the Open command in the File menu. Hardware items are identified in the text by bold text (e.g., Power switch). ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 10: The Imagequant Las 4000

    2 The ImageQuant LAS 4000 2 The ImageQuant LAS 4000 This chapter describes the features and part names of the ImageQuant LAS 4000 hardware, and the connectors that connect the various parts of the equipment. 2.1 The ImageQuant LAS 4000 exterior...

  • Page 11: Inside The Imagequant Las 4000

    Sample tray Supports the sample Lifting table Moves the sample to the selected tray position Lens F0.85/43 mm LAS High Sens. lens, or F1.8/24 mm wide view lens Lens connector Connects lens to IDX ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 12: Connections

    Do not connect anything other than the transmitted light sources to the transmitted light source connector. 2.3 Connections Camera head Name Camera cable USB cable Do not connect cables other than the camera cable supplied with the equipment. Note: ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 13 • Be sure to use Epi light sources of the same type for the right and left sides other- wise an incorrect image will be obtained. • Do not insert any connector other than those for the Epi light sources. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 14: Parts And Accessories

    • Do not connect the instrument and the PC via a USB hub. • Do not connect USB equipment other than ImageQuant LAS 4000 to the PC. Simi- larly, do not use other USB equipment connected to the PC while ImageQuant LAS 4000 is in use.
  • Page 15 Cal plate GR (Pink) Cal plate DI Cal plate FL (Green) NP tray White trans tray UV trans tray Gel sheet White light table UV transilluminator F-mount adapter F1.8 24mm wide view lens ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 16 • The UV trans tray will degrade after many UV illuminations. It can be used up to approximately 1000 times if each exposure time is 1 second. New trays can be purchased separately. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 17: Exchanging Accessory Parts

    Optical filters are installed in the filter turret localted under the lens inside the IDX. This section describes how to install or change a filter on the filter turret. Install a filter in the filter turrent and register it in the ImageQuant LAS 4000 Control Software as follows: 2, 5 1 Ensure that the camera head and PC are connected.

  • Page 18: Installing Or Exchanging The Lens

    3.2 Installing or exchanging the lens Two lenses can be used with the ImageQuant LAS 4000, an F0.85 43mm LAS High Sens. lens, and an optional F1.8 24mm wide view lens. The wide view lens can image samples up to 250 x 250 mm in size.

  • Page 19: Removing The Las High Sens. Lens

    1 Close the instrument door. If the power is turned off and the filter changer is down, proceed to step 2 Select Option:Lens Exchange. A message is displayed. 3 Click OK. Turn off the instrument using the power switch. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 20 6 Disconnect the lens cable connector by rotating the knurling ring. Knurling ring Connector of the lens cable 7 Turn the lens slightly to the left and loosen it while pushing the lens release lever. Lens release lever ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 21 Hold the lens with both hands so as to prevent the lens guide from being damaged. 9 Take out the lens slowly along the lens guide toward you. 10 Place the end cap on. End cap ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 22: Installing The Las High Sens. Lens

    Click OK. Turn off the instrument using the power switch. 4 Remove the end cap from the lens. 5 Insert the lens along the lens guide with the red points facing towards you. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 23 Turn the knurling ring until it is securely fixed. Knurling ring Connector of the lens cable Note: Be sure to connect the lens cable. Otherwise, the high sensitivity lens cannot be recognized. This may cause malfunction of the equipment. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 24: Removing The Wide View Lens

    9 Install the lens hood. Lens hood 3.2.3 Removing the wide view lens 1 Close the instrument door. If the power is turned off and the filter changer is down, proceed to step 2 Select Option:Lens Exchange..ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 25: Installing The Wide View Lens

    8 Place the end cap on the lens and the cap on the F-mount adapter. 3.2.4 Installing the wide view lens 1 Close the instrument door. If the power is turned off and the filter changer is down, proceed to step 2 Select Option:Lens Exchange..ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 26 6 Align the White point mark of the F-mount adapter with the lens mark and turn to lock them together. Hold the lens so that the red point is towards you. F-mount adapter Wide view lens ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 27: Changing The Light Sources

    LAS 4000. This section describes how to change both types of light source. The following table summarizes the properties of the various light sources. Type Name Peak wavelength UV transilluminator 312 nm Transmitted White light table ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 28: Changing The Uv Transilluminator Or White Light Table

    1 If neither the UV transilluminator or white light table are currently in place inside the instrument, proceed to step 4. 2 Turn the lever holding the light source to unlock it. 3 Pull out the light source carefully along the guide while holding it with both hands. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 29: Changing The Epi Lights

    The tray should be at tray position 4 before installing Epi lights. If the tray is higher, then it should be lowered to tray position 4 using the ImageQuant LAS 4000 Control Software before attaching the lights, as described below.
  • Page 30 5 Holding the latch with one hand, remove the Epi light by pulling it gently outwards. 6 Lift up and remove the Epi light. 7 Before installing the new Epi light, ensure that the latch is sprung open (upwards). ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 31 • The Epi lights are clearly labelled L for the left and R for the right sides. • Install the Epi lights in matching pairs only. If Epi lights with different wavelengths are used, the intended image will not be obtained. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 32: Operating The Imagequant Las 4000

    4.1 Layout of the ImageQuant LAS 4000 Control Software The exposure of samples in the ImageQuant LAS 4000, the setting of exposure conditions and the viewing of the digitized images are performed within the ImageQuant LAS 4000 Control Software.

  • Page 33: Preparation For Exposure

    Turn on the ImageQuant LAS 4000, computer and peripheral equipment. Start up ImageQuant LAS 4000 Control Software. A message is displayed until the ImageQuant LAS 4000 is ready. The power switches of the instrument and PC can be turned on in any order.

  • Page 34: Calibration

    • The Start button can be clicked even when the CCD is not completely cooled with the EtBr trans method. 4.3 Calibration Ensure that the ImageQuant LAS 4000 is properly calibrated. See Section 5.12.1 CCD Calibration, on page 104 Section 5.12.2 Flat Frame Calibration, on page 105.
  • Page 35 Sample Gel sheet The gel sheet can be used repeatedly. After use, wash it with mild detergent, rinse Note: with water then dry well. A gel sheet can be reused about 20 times. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 36 Line up the sample using the appropriate dents. For NP tray • The NP tray is to be used at tray position 3. Note: The readable area will be slightly smaller using the F1.8 wide view lens. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 37: Exposing Chemiluminescent Samples

    This section describes how to take images of chemiluminescent samples. No illumination is used for this application. Place a sample on the Epi tray. Put the tray in the ImageQuant LAS 4000 then close the door. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 38 4.5 Exposing chemiluminescent samples Step Action Click the Method/Tray position button. Result: The Method/Tray position dialog opens. • Select Chemiluminescence for Method. • Select Tray position according to the sample. • Click the OK button. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 39 Check that the sample is correctly postioned and focus the image. Click the Return button. Click on the image to magnify it. Click on the image again to return to the original TIP: size. Select Precision for Exposure Type. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 40 The exposure time can be set from 0.01 seconds up to 30 hours. Automatic exposure Note: time setting may not be possible de- pending on the sample type and method. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 41 Select Help:Sensitivity/Resolution..This opens the following diagram that de- TIP: scribes the relation between sensitivity and resolution. Click the Start button. Result: The exposure is started. The orange Busy LED lights on the instrument during exposure. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 42: Exposing Fluorescent Samples (Etbr)

    Place a gel sheet, which has been cut out to be slightly larger than the sample, onto the UV trans tray. Place the sample on the gel sheet. Put the tray into the ImageQuant LAS 4000 and close the door.
  • Page 43 Result: The Method/Tray position dialog opens. • Select Fluorescence as the Method, and choose EtBr from the pull-down menu. The 605DF40 filter is automatically selected. • Select the Tray position. • Click the OK button. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 44 Confirm the sample position and focus. Click the Return button. Click on the image to magnify it. Click on the image again to return to the original TIP: size. Select Precision for Exposure Type. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 45 Select Sensitivity/Resolution. Select Help:Sensitivity/Resolution..This opens the following diagram that de- TIP: scribes the relation between sensitivity and resolution. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 46 The display returns to the main screen. TIP: If the Method EtBr UV (Trans-illumination) is used, the Start button can be clicked and exposure taken even if the temperature setting state of the CCD is Not Ready. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 47: Exposing Fluorescence (Epi Illumination)

    Chapter 3 Exchanging accessory parts, on page Place a sample on the Epi tray. Put the tray into the ImageQuant LAS 4000 and close the door. Note: • Make sure that sample is suitably placed on the tray for the desired tray position.
  • Page 48 • Select Fluorescence as the Method, choose the appropriate fluorescent marker and the ap- propriate light source from the pull-down menus. The appropriate filter is automatically selected. • Select the Tray position. • Click the OK button. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 49 Confirm the sample position and focus. Click the Return button. Click on the image to magnify it. Click on the image again to return to the original TIP: size. Select Precision for Exposure Type. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 50 Select “Sensitivity/Resolution...” in the Help menu. You can display Help that de- TIP: scribes the relation between sensitivity and resolution. Click the Start button. The orange Busy LED lights on the instrument and a progress dialog is displayed during exposure. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 51: Exposing Dye Stained Samples And Films (White Epi Light)

    (Trans illumination), on page 55 for a more optimal method. Place a sample on the Epi tray. Put the tray in the ImageQuant LAS 4000 then close the door. Note: • Prior to exposure, click on the Digitiza-...
  • Page 52 Action Click the Method/Tray position button. Result: The Method/Tray position dialog opens. • Select Digitization and Epi-illumination for Method. • Select Tray position according to the sample used. • Click the OK button. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 53 Confirm the sample position and focus. Click the Return button. Click on the image to magnify it. Click on the image again to return to the original TIP: size. Select Precision for Exposure Type. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 54 Select “Sensitivity/Resolution...” in the Help menu. You can display Help that de- TIP: scribes the relation between sensitivity and resolution. Click the Start button. The orange Busy LED lights on the instrument and a progress dialog is displayed during exposure. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 55: Exposing Dye Stained Samples And Films (Trans Illumination)

    4.9 Exposing dye stained samples and films (Trans illumination) This section describes how to expose dye-stained samples using white trans illumination. Place a sample on the White trans tray. Put the tray in the IDX and close the door. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 56 4.9 Exposing dye stained samples and films (Trans illumination) Step Action Click the Method/Tray position button. Result: The Method/Tray position dialog opens. 1 Select Digitization:Trans-illumination for Method. 2 Select Tray position according to the sample used. 3 Click the OK button. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 57 Confirm the sample position and the focus. Click the Return button. Click on the image to magnify it. Click on the image again to return to the original size. TIP: Select Precision for Exposure Type. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 58 Select “Sensitivity/Resolution...” in the Help menu. You can display Help that describes TIP: the relation between sensitivity and resolution. Click the Start button. The orange Busy LED lights on the instrument and a progress dialog is displayed during exposure. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 59: Exposing Consecutively (Increment)

    This section describes how to perform this type of experiment. Place a sample on the appropriate tray. Place the tray in the ImageQuant LAS 4000 then close the door.
  • Page 60 4 Operating the ImageQuant LAS 4000 4.10 Exposing consecutively (Increment) Step Action Click the Method/Tray position button. Result: The Method/Tray position dialog is opened. Select the Method and Tray Position. Click the OK button. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 61 Click on the image to magnify it. Click on the image again to return to the original TIP: size. Select Increment for Exposure Type. Select Interval Time unit and enter a numeric value. Interval Time can be entered in the range of 10 seconds to 2 hours. Note: ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 62 Select “Sensitivity/Resolution...” in the Help menu. You can display Help that de- TIP: scribes the relation between sensitivity and resolution. To specify the maximum number of exposures to be taken, check the Set Limit check box and specify the number of images. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 63 The changed gradation is ap- dragging the mouse. plied to all images. The gradation conversion Return to initial setting. curve can be toggled between Linear or Sigmoid. The gradation can be changed by dragging the mouse. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 64: Exposing Repeatedly (Repetition)

    Place a sample on the tray that you selected according to the type of a sample. Put the tray in the ImageQuant LAS 4000 then close the door. Note: • Make sure that the tray is placed at the required position.
  • Page 65 4 Operating the ImageQuant LAS 4000 4.11 Exposing repeatedly (Repetition) Step Action Click the Method/Tray position button. Result: The Method/Tray position dialog is opened. Select the Method and Tray Position. Click the OK button. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 66 Click on the image to magnify it. Click on the image again to return to the original TIP: size. Select Repetition for Exposure Type. Select Interval Time unit and enter a numeric value. Interval Time can be entered in the range of 10 seconds to 2 hours. Note: ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 67 Select “Sensitivity/Resolution...” in the Help menu. You can display Help that de- TIP: scribes the relation between sensitivity and resolution. To specify the maximum number of exposures to be taken, check the Set Limit check box and specify the number of images. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 68 The changed gradation is ap- dragging the mouse. plied to all images. The gradation conversion Return to initial setting. curve can be toggled between Linear or Sigmoid. The gradation can be changed by dragging the mouse. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 69: Printing Exposed Images

    Select the output setting for the printer and the type (full-scale print, paper-size print or screen print). Click the OK button. Full-scale print Paper-size print Screen print The Print dialog is displayed. Choose settings for the printer and click the Print button. The image is printed. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 70: Saving Exposed Images

    Control Software version number. Nov Wed 25 15:25:14 2009 Serial#1234567 FUJINON VRF43LMD Cooling:-25C Precision Standard 1sec Chemiluminescence Tray Position 1 Range:512-4607 Gradation:512-4607 Linear ImageQuant LAS 4000 mini 1.1.0.17 Image 4.13 Saving exposed images The exposed image can be saved using the Save button displayed in the post-exposure state or by File:Save.
  • Page 71 Make Increment Image (Exposure Type: Program only) Files of multiple exposures are accu- mulated and saved. The range of image files to be pro- cessed in accumulation can be speci- fied. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 72: Ending The Session

    Windows should not be used in a file name. 4.14 Ending the session This section describes how to end the current session with the ImageQuant LAS 4000. The instrument should be switched off as described below when it will not be used for some time.

  • Page 73: Exposing Large Sample Using Wide View Lens

    CCD will be maintained and the instrument can be used straight away the next time the ImageQuant LAS 4000 Control Software is started. In this case, do not turn off the ImageQuant LAS 4000. However, if the instrument will not be used for a long period, it should be turned off.
  • Page 74 4.15 Exposing large sample using wide view lens Step Action Place a sample on the tray that you selected according to the light source used. Put the tray in IDX and close the door. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 75 1 Select the Method, Light source, Filter, Iris, and Tray position. 2 If FlatFrame Mode is set to manual in Edit:Preference, click the Next button. 3 Select the appropriate Flat Frame file. 4 Click the OK button. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 76 Click the Return button. Click on the image to magnify it. Click on the image again to return to the original size. TIP: Select Exposure Type. Set the Exposure Time or Interval Time. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 77 Click the Start button. Exposure is started. Adjust the gradations of the exposed image, then save and print the image. Click the Complete button. The display returns to the main screen. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 78: Creating A New Method And Performing Flat Frame Calibration

    Select the appropriate Light, Filter and Iris, edit the Name for the method and click OK. Once the method is added to the list of methods, click OK to return to the main window. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 79 4 Operating the ImageQuant LAS 4000 4.16 Creating a new method and performing flat frame calibration Step Operation Insert the calibration plate appropriate for the light source into the ImageQuant LAS 4000. Light source Calibration plate Red Epi light Cal plate GR (pink)

  • Page 80: Software Reference Guide

    Exposes for each interval time set in Interval Time and displays images for each section. Program Exposure is made for the desired time and exposure intervals. A file is created automatically for each read image, and the file of accumulated images is also saved automatically. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 81: Exposure Time

    5 Software reference guide 5.1 Exposure type 5.2 Exposure time This function is for setting the exposure time. When exposure type is precision • Auto: The exposure time is automatically calculated by histogram analysis. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 82: Add Digitization Image

    A chemiluminescence and a white-light epi-illuminated image are exposed by single-click operation when this function is set to ON. After exposure, both chemiluminescence and white epi-illuminated images can be saved. This function can be used only when the method is chemiluminescence. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 83: Program Settings

    Invert Pixel-data. 5.4 Program settings Program Settings are used to set exposure time and exposure intervals when Program mode is selected as Exposure Type. The input settings are saved as a template file. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 84: Focusing

    C:\GE Healthcare\ImageQuant LAS 4000\ProgramTemplate 5.5 Focusing The Brightness function enables adjustment of the brightness of the Focusing screen, and the Adjust function enables adjustment of the focus when the LAS High Sens. lens is used. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 85: Start

    • When the NP tray is used, adjust so that the bottom of a plate is focused. Adjustment using a printed paper facilitates focusing. • Check the tray positions to be exposed in advance. 5.6 Start Exposure starts when you click the Start button. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 86 5.6 Start During exposure (type Precision) During exposure, a progress bar and ending time are displayed to indicate the progress status of exposure. Click Cancel to discontinue exposure. The current image is not saved. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 87 • When you click the Apply All button during magnified image display, the gradation changed in the expanded image display state is applied to all magnified images. Note: Only the 100 latest exposed images can be saved for exposure types Increment and Repetition. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 88 • An image, which is the accumulation of all exposed images is saved in a file with name format: __ Note: Only the 16 latest exposed images can be saved for exposure type Program. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 89 The range can be changed by dragging the Print image or screen. mouse. Gradation can be changed by dragging the Save image. mouse. The gradation conversion curve can be Return to main screen. changed (linear or sigmoidal). ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 90: Sensitivity/Resolution

    Click on an image to select it. Double- click an image or click the Image tab to open the magnified image view. Returns the gradation to the initial setting. 5.7 Sensitivity/Resolution This function sets the sensitivity and resolution. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 91: Method/Tray Position

    The window for setting the method and tray position is displayed when you click the Method/Tray position button. Select a method and tray position most suitable for the sample and click the OK button. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 92 UV (312nm Trans-illumi- 605DF40 F2.8 nation) Blue (460nm Epi) Y515 Di F0.85 Fluorescence Green (520nm Epi) 575DF20 F0.85 Red (630nm Epi) R670 F0.85 Dy781 NIR (710nm Epi) IR785 F0.85 DAPI UV (365nm Epi) F2.8 ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 93 • The NP tray is to be used at tray position 3 using the Chemiluminescence method and the NP check box should be checked. The tray will not be detected otherwise. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 94: File Menu

    Opens the settings for the printer, for example the direction of paper. Print Outputs an image to a printer. The image can be printed as a full-scale print or screen print. For the output method, see Section 4.12 Printing exposed images, on page ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 95: Edit Menu

    Copies the selected characters. This function can be used during manual input of exposure time and interval. Paste Pastes the copied characters. This function can be used during manual input of exposure time and interval. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 96 5 Software reference guide 5.10 Edit menu Preference The available settings can be viewed by clicking the respective item. CCD Cooling Sets the CCD temperature. Root Folder Sets the folder in which files are saved. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 97 • Original Image TIFF File-Linear 16bit Gray (*.tif) This is a 16-bit TIFF format. • Window Image TIFF File-8bit Color (*.tif) This is a TIFF format that has an eight-bit color gradation. The changed gradation can be saved directly. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 98 Optimal correction data is automatically set. Manual Correction data can be selected in the Flat Frame selection screen. Launch Applicaton This option can be used to automatically launch an external application when an image is saved. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 99 • Select the application to be launched from the drop down list, or locate the application using the Select... button. • Check the Activate save and launch function checkbox. Print Adjust Adjusts the print output size. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 100 5.10 Edit menu Increment Option Description During incremental imaging, exposure is automatically stopped when the amount of satu- rated data is too large. During incremental imaging, exposure is continued until the Stop button is pressed. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 101 5 Software reference guide 5.10 Edit menu Digitization EPI Option Description Pixel data inversion is performed. Pixel data inversion is not performed. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 102 5 Software reference guide 5.10 Edit menu Timer If the ImageQuant LAS 4000 is not used during the chosen time, the cooling will be automatically shut off and the system will enter power saving mode. Auto Range An image whose gradations are adjusted automatically is displayed when exposure type is Increment or Repetition.

  • Page 103: View Menu

    This sets the display color of an image such that illuminated regions are displayed in blue. Pan Focus All This function toggles the quality of a focus image. The image is displayed in high image quality when this option is selected. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 104: Option Menu

    2 hours. resolutions for more than 2 hours. The time required for creation is dis- Click on the buttons in this column to played. check the boxes for both correction files for specific resolutions. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 105: Flat Frame Calibration

    Make sure that an appropriate filter and tray are in place. 6 A message window opens when the calibration is completed. Click OK. 7 Click the Close button to close the FlatFrame Calibration window. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 106: Method Setting

    Only user-defined methods can be deleted. • To edit a method, select the method to be edited and click Edit. Note: Only user-defined methods can be edited. • To add a method, click Add. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 107: Help Menu

    Opens the ImageQuant LAS 4000 User Manual in the default pdf browser. Getting Started Opens the Getting Started with ImageQuant LAS 4000 manual in the default pdf browser. End-User License Agreement Opens the End-User License Agreement in the default pdf browser.
  • Page 108 5 Software reference guide 5.13 Help menu About ImageQuant LAS 4000 Control Software The version information of Image Reader is displayed. Click to close the window. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 109: Installing Imagequant Las 4000 Control Software

    Software installation is performed in the following sequence: 1 Install the USB contol driver 2 Install the USB function driver 3 Install the ImageQuant LAS 4000 Control Software 6.2 Install ImageQuant LAS 4000 Control Software under Windows XP Before you begin Log in using a Windows account with administrator privileges.
  • Page 110 Select Show All Devices and click the Next button. Click the Have Disk button in the Add hardware wizard. Insert the ImageQuant LAS 4000 Control Software CD and click the Browse button. Select to install the driver from the ImageQuant LAS 4000 Control Software CD.
  • Page 111 6 Installing ImageQuant LAS 4000 Control Software 6.2 Install ImageQuant LAS 4000 Control Software under Windows XP Step Action Open the USB Control folder. Select the DevMng.inf file and click the Open button. Click the OK button in the Install from disk dialog.
  • Page 112 Install the USB function driver (Windows XP) Step Action Connect the computer and the ImageQuant LAS 4000 with a USB cable and turn ON the power switch of ImageQuant LAS 4000. The scanner is automatically detected by the computer. In the Found New Hardware Wizard dialog, choose No, not this time.

  • Page 113: Install Imagequant Las 4000 Control Software Under Windows Vista

    • Click the Change button to install to a different folder. Click the Install button in the installation dialog. Click the Finish button. Start the ImageQuant LAS 4000 Control Software. This will ensure that necessary folders and files are created. 6.3 Install ImageQuant LAS 4000 Control Software under...
  • Page 114 Select Show All Devices and click the Next button. Click the Have Disk button. Insert the ImageQuant LAS 4000 Control Software CD and click the Browse button. Select to install the driver from the ImageQuant LAS 4000 Control Software CD.
  • Page 115 6 Installing ImageQuant LAS 4000 Control Software 6.3 Install ImageQuant LAS 4000 Control Software under Windows Vista Step Action Select the USB Control folder and click Open. Select the file DevMng and click the Open button. Click the OK button in the dialog Install from disk.
  • Page 116 Continue to proceed with the installation. Step Action Connect the computer and the ImageQuant LAS 4000 with a USB cable and turn ON the power switch of ImageQuant LAS 4000. Result: The scanner will automatically be detected by the computer and the Plug and Play function in Windows Vista starts.
  • Page 117 An unidentified program wants access to your computer. Click Allow. Click the Finish button. The installation of ImageQuant LAS 4000 Control Software is now completed. Start the ImageQuant LAS 4000 Control Software. This will ensure that necessary folders and files are created. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 118: Uninstalling And Upgrading Imagequant Las 4000 Control Software

    Open the control panel and select Add or Remove Programs. Select ImageQuant LAS 4000 and click the Remove button. Correction files created during calibration are required by the ImageQuant LAS 4000 Note: Control Software. They are not deleted during the uninstallation, and remain in the Data folder of ImageQuant LAS 4000 Control Software folder.
  • Page 119 An unidentified program wants access to your computer. Click Allow. Upgrading ImageQuant LAS 4000 Control Software Step Action Uninstall the current version of ImageQuant LAS 4000 Control Software. Install the new version of ImageQuant LAS 4000 Control Software. ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 120: After-Sales Service

    1 The warranty period will expire after 1 year from the date the system was delivered. 2 GE Healthcare will make repairs free of charge for failures during the warranty period, provided that normal usage conditions and the instructions given in this manual, etc., are followed.

  • Page 121: A Appendix

    Under the same conditions (tray position, iris, fluorescent filter, light source, and tray) as when exposing an object sample, a flat frame records the image in a uniform fluorescent plate (fluorescent mode) or white plate (digitize mode). ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 122: Quick-Reference Functions

    Conceptual diagram of CCD calibration and flat frame A.2 Quick-reference functions Function Function name Set item Exposing for a fixed exposure Exposure Type Precision time Exposing consecutively Exposure Type Increment Exposing repeatedly Exposure Type Repetition ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 123 Method Chemiluminescence Fluorescence:EtBr Fluorescence:SYBR Green Fluorescence:GFP Fluorescence:Cy3 Selecting detection method Fluorescence:Cy5 Fluorescence:Dy781 Fluorescence:DAPI Digitization:DIA Digitization:EPI Method/Tray position Select the tray position from 1 Changing tray position to 4. ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 124 Option:Flat Frame Calibration Select and set conditions for Creating Flat Frame Start Checking software version in- Help:About ImageQuant LAS Select formation 4000 Control Software Checking the relation between Help:Sensitivity/Resolution Select sensitivity and resolution ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 125: Detection Reagents And Corresponding Settings

    Lumi-Light Plus Chemiluminescence none Through 0.85 Renaissance Chemiluminescence none Through 0.85 Chemiluminescence Super Signal Chemiluminescence none Through 0.85 Bright-Star Chemiluminescence none Through 0.85 CDP-Star Chemiluminescence none Through 0.85 CSPD Chemiluminescence none Through 0.85 ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 126 Green (520nm 575DF20 0.85 Epi) Fluorescence:Cy3 Green (520nm 575DF20 0.85 Epi) Fluorescence:Cy5 Red (630nm R670 0.85 Epi) Alexa 633 Fluorescence:Cy5 Red (630nm R670 0.85 Epi) Alexa 660 Fluorescence:Cy5 Red (630nm R670 0.85 Epi) ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 127: Main Specifications

    Number of gradations Recorded image: 16 bit Focusing: 8 bit Exposure time 1/100 seconds to 2 hours (Images can be continuously exposed for up to 30 hours using ImageQuant LAS 4000 Control Software.) ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 128 : 224 x 161 x 252 mm (W/H/D) : 510 x 730 x 480 mm (W/H/D) Weight Camera head : 3.4 kg : 49 kg (not including the light source, lens, and tray) High-sensitivity lens : 4.5 kg ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 129 : 50/60 Hz Rated input current : 3.0-1.5 A AC power cable Use the cable supplied with the instrument. Specifications of cables required for the use of ImageQuant LAS 4000 Voltage : 100-120 V Plug/connector : 125 V AC, 13 A Cable : SJT3 x 16AWG 60°C...
  • Page 130 A Appendix A.4 Main specifications Installation conditions Placement conditions Free space required around ImageQuant LAS 4000 Secure space for maintenance work as follows: Front : 600 mm Right : 600 mm Left : 600 mm Rear : 200 mm : 1000 mm...
  • Page 131 Rated pollution applied : Pollution Degree 2 Analysis unit interface USB 2.0 Do not connect the ImageQuant LAS 4000 USB connector to a computer not certified with UL60950-1 (UL listed) and IEC60950-1. Others Noise : 70 dB (A) or lower...

  • Page 132: Minimum Computer Requirements

    Windows Vista™ Business SP1 (32-bit) Memory 1 GB or more Processor Intel Core 2 Duo processor 80 GB or more USB port version USB 2.0 Optical drives DVD-ROM Monitor resolution 1280 × 1024 pixels or more ImageQuant LAS 4000 User Manual 28-9607-42 AB...

  • Page 133: Service Report Fax Sheet

    ImageQuant LAS 4000 system information 6) Serial No. • Camera head • Others 7) Analyzing unit type Windows • Operating environment • Machine model 8) Usage frequency 9) Trouble occurrence frequency 10) Error code and failure description ImageQuant LAS 4000 User Manual 28-9607-42 AB...
  • Page 136 First published Oct. 2009 Björkgatan 30 All goods and services are sold subject to the terms and conditions of sale of the company within GE 751 84 Uppsala Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare representative for the most current information.

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