Page 1 INSTRUCTION MANUAL 889-9231-008 Ver.9 June. 2017 Ultra High Performance Liquid Chromatograph ChromasterUltra R 6430 DIODE ARRAY DETECTOR...
Page 2 Hitachi High-Tech Science Corporation. 3. Hitachi High-Tech Science Corporation assumes no liability for any direct, indirect, or consequential damages arising from use not described in this manual.
Page 3: Preface
PREFACE Thank you very much for purchasing the ChromasterUltra R System. The ChromasterUltra R System is designed to perform liquid chromatography which separates and detects the existence in a sample. Note that physiologically or biologically active samples are not applicable to the ChromasterUltra R System because of possible infection with bacteria or viruses.
Page 4: About This Manual
ABOUT THIS MANUAL This instruction manual describes how to use and maintain the ChromasterUltra R System. This instruction manual consists of the following sections. IMPORTANT (Warranty, Installation, Relocation, After-sale Technical Service, etc) SAFETY SUMMARY (collected and summarized) Section 1 OUTLINE (Outline of the ChromasterUltra R System) Section 2 FUNCTIONS...
Page 5: Important
IMPORTANT Warranty on Product Limited Warranty The ChromasterUltra R System is warranted to be free from defects in material or workmanship under normal use within the product specifications indicated in this manual and under conditions given below. This warranty is void if the software is not used according to the instruction manual.
Page 6 Warranty Limitations and Exclusions Disclaimer of Warranty THE MANUFACTURER MAKES NO WARRANTIES, EITHER EXPRESS OR IMPLIED, EXCEPT AS PROVIDED HEREIN, INCLUDING WITHOUT LIMITATION THEREOF, WARRANTIES AS TO MARKETABILITY, MERCHANTABILITY, FOR A PARTICULAR PURPOSE OR USE, OR AGAINST INFRINGEMENT OF ANY PATENT. IN NO EVENT SHALL THE MANUFACTURER BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT.
Page 7: Installation, Relocation And After-Sale Technical Service
(m) Failure due to a life-limited part that has exceeded the end of its useful lifetime. Limitations of Liability Hitachi High-Tech Science shall not be liable in contract or in tort (including, without limitation, negligence, strict liability or otherwise) for claims by third parties (other then for bodily injury or...
Page 8: Technical Seminars And Training Courses For Users
After-sales Service (a) For after-sale technical service of this instrument, please notify our local sales representative or service office. (b) A maintenance & service contract is available for servicing the instrument after the warranty period has ended (service available at charge). (c) Maintenance parts and consumables will be supplied during the useful life of this instrument (7 years).
Page 9: Caution On Disposal Of Instrument
Note, however, that the environmental protection laws and regulations may be revised or amended. Therefore, be sure to consult with your local Hitachi High-Technologies sales or service representative when planning disposal of this instrument or its parts and accessories.
Page 10: Information For Users On Weee (Only For Eu Countries)
Information for Users on WEEE (only for EU Countries) This symbol is in compliance with the Waste Electrical and Electronic Equipment directive 2012/19/EU (WEEE). This symbol on the product indicates the requirement NOT to dispose of the equipment as unsorted municipal waste, but use the return and collection systems available.
Page 11 Disturbance by Electromagnetic Wave This instrument conforms to Class A in the EN standards EN61326-1 (2013). Avoid installing this instrument near equipment whose data will be affected by electromagnetic noise within the permissible range of this standard. Also, data of this instrument may be affected by electromagnetic noise or the instrument itself may malfunction.
Page 12: Safety Summary
Do not perform any operation or action other than described in these manuals.  On occurrence of any trouble in the instrument, notify the nearest sales service representative of Hitachi High-Technologies.  Keep in mind that the hazard warnings in the manuals or on the product cannot cover every possible case, as it is impossible to predict and evaluate all circumstances beforehand.
Page 13: General Safety Precautions
Disconnect the power cord plug from the power outlet. Then, after providing proper safety measures as required, contact the nearest service representative of Hitachi High-Technologies. Using the instrument in such an abnormal condition could result in an electric shock or fire.
Page 14: Warning Precautions In The Manual
SAFETY SUMMARY WARNING precautions in the Manual WARNING Ignition of Flammable Chemicals  This instrument is not explosion-proof. In unattended operation, do not use organic solvents having an ignition point below 70 C (Manual System Section 3) (Manual Binary Pump Section 4) (Manual Autosampler Section 5) (Manual Column Oven Section 2) (Manual UV-VIS Section 5) (Manual DAD Section 5) (Manual Organizer Section 3) (Manual FL Section 5)
Page 15 SAFETY SUMMARY WARNING precautions in the Manual (Continued) WARNING Explosion of Vapor from Flammable Chemicals If a flammable chemical such as organic solvent leaks from the flow path of the instrument and its vapor concentration reaches the explosion limit, it could cause spontaneous combustion with dangerously explosive results.
Page 16 SAFETY SUMMARY WARNING precautions in the Manual (Continued) WARNING Electric Shock due to Improper Grounding This instrument is designed in conformity with the specifications of Class I in Annex H of the IEC 61010-1 (International Electrotechnical Commission Standards) – Issue 1. To prevent an electric shock hazard, provide a proper grounding connection.
Page 17 SAFETY SUMMARY WARNING precautions in the Manual (Continued) WARNING Injury of Hand by Needle or Internal Mechanism Never touch the inside of the autosampler during operation, as your hand or finger may be seriously injured by the needle or internal mechanism. (Manual Autosampler Section 4.2) Prohibition of Releasing the Safety Mechanism In this instrument, the safety mechanism functions to turn off power supply automatically...
Page 18 SAFETY SUMMARY WARNING precautions in the Manual (Continued) WARNING Injury due to Xenon Lamp Explosion [In use]  When the cumulative turn-on time of the xenon lamp exceeds the guaranteed useful lifetime, the electrodes evaporate and the scattered matter sticks to the bulb wall, so its blackening progresses and heat dissipation is hindered.
Page 19 SAFETY SUMMARY WARNING precautions in the Manual (Continued) WARNING Injury due to Xenon Lamp Explosion [At mounting] (Continued)  Do not touch the quartz glass part of the xenon lamp with bare hands. If the quartz glass part of the xenon lamp is contaminated with dust or fingerprints, wipe it using a gauze sheet or absorbent cotton cloth slightly moistened with high-quality alcohol.
Page 20: Caution Precautions In The Manual
SAFETY SUMMARY CAUTION Precautions in the Manual CAUTION Caution in Carrying Heavy Instrument  ChromasterUltra R Organizer weighs as much as 9 kg. Be careful not to drop it and to handle it with care when carrying it, for preventing injury. Hold the right and left parts of the instrument firmly.
Page 21 SAFETY SUMMARY CAUTION Precautions in the Manual (Continued) CAUTION Prevention of Instrument Tipover Personal injury could be incurred if the instrument tips over. When installing the instrument, provide a proper means for preventing the instrument from tipping over accidentally. (APPENDIX 1) Fatigue due to Long-Hour Operation If you keep working with the display monitor and keyboard for long hours, your eyes and body will be fatigued to jeopardize your health.
Page 22 SAFETY SUMMARY CAUTION Precautions in the Manual (Continued) CAUTION  The light source lamp and its cover become hot during operation and remain hot for a while even after power-off. They can severely burn you if touched. Before replacement of the lamp, turn power off and wait for about 60 minutes until it cools down sufficiently.
Page 23 If any one of the warning labels becomes s illegible due to deterioration, contact your lo ocal Hitachi High-Technologies Corporat tion sales representative or service office of H Hitachi High-Technologies Corporat tion sales representative for replacement with h a new one.
Page 24 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument ChromasterUltra R 6170 Bi inary Pump (1) Ignition of Fla ammable Chemicals (2) Explosion of Vapor from Flammable Chemicals Left sid de view (3) Electric Shoc ck due to Contact with Inside of f Instrument Rear Pan...
Page 25 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument ChromasterUltra R 6270 Au utosampler (1) Ignition of Flamm mable Chemicals (2) Explosion of Vap por from Flammable Chemicals Left side v view (3) Electric Shock d due to Contact with Inside of Ins strument Rear Pan...
Page 26 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument (4) Damage to Needle by Misss setting Sample Rack Inside View (L Left Side) (5) Injury of Hand by Needle or r Internal Mechanism Front Vi SAFETY - 15...
Page 27 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument ChromasterUltra R 6310 Co olumn Oven (1) Electric Shoc k due to Contact with Inside of f Instrument Rear Panel SAFETY - 16...
Page 28 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument (3) Do Not Touch H Hot Part (2) Ignition of Flammable Che emicals (4) Explosion of Va apor from Flammable Chemicals Right side e view SAFETY - 17...
Page 29 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument ChromasterUltra R 6420 UV V-VIS detector (1) Ignition of Flammable e Chemicals (2) Explosion of Vapor from F Flammable Chemicals Left side view (3) Electric Shock due to Contact t with Inside of Instrument ar Panel SAFETY - 18...
Page 30 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument (4) Electric Shock due to Cont tact with Inside of Instrument (5) Do Not Touc h Hot Part Front View SAFETY - 19...
Page 31 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument ChromasterUltra R 6430 Di iode Array detector (1) Ignition of Flammable e Chemicals (2) Explosion of Vapor from m Flammable Chemicals Left side view (3) Electric Shock due to Contact with Inside of Instrument ar Panel SAFETY - 20...
Page 32 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument (5) Burns due to Contact w ith Hot Parts (4) Electric Shock due to Con ntact with Power Supply to Light Source Front View SAFETY - 21...
Page 33 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument ChromasterUltra R 6440 FL L detector (1) Explosion of Vapor fro om Flammable (2) Electric Shock due to Co ontact with Light Chemicals Source Power Supply Front View Explosion of Xenon (3) Ignition of Flammable Chemic cals (4) Burns due to Contact with Hot Parts...
Page 34 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument (6) Elect ric Shock due to Conta act with Inside of Instru ument Rear Panel SAFETY - 23...
Page 35: Notice Precautions
SAFETY SUMMARY NOTICE precautions Precautions on Use of Solvents  Alumina, EPDM (ethylene-propylene rubber), ETFE (Ethylene tetrafluoroethylene), FEP (fluorinated ethylene-propylene), FFPM (Perfluoroelastomer), PCTFE (polychlorotrifluoroethylene), PEEK (polyether ether ketone), PP (polypropylene), PPS (Polyphenylene sulfide), PTFE (polytetrafluoroethylene), PVDF (polyvinylidene fluoride), Quartz glass, Ruby, Sapphire, SUS316 (Stainless steel), Teflon®AF, UHMWPE (ultra high molecular weight polyethylene), Vespel®...
Page 36 SAFETY SUMMARY NOTICE precautions (Continued)  Referring to the instruction manual about each module unit of the liquid chromatograph system, set up the automatic liquid feed stop function (such as the pressure limiter function of the pump unit) to prevent a possible trouble. For detailed information, refer to the instruction manual about the ChromasterUltra R 6170 Binary Pump.
Page 37: Table Of Contents
CONTENTS PREFACE ..........................1 ABOUT THIS MANUAL ······················································································· 2 IMPORTANT ···················································································· IMPORTANT-1 Warranty on Product ··································································· IMPORTANT-1 Installation, Relocation and After-sale Technical Service ·················· IMPORTANT-3 Technical Seminars and Training Courses for Users ······················· IMPORTANT-4 Estimated Life time of the Instrument ············································ IMPORTANT-4 Caution on Disposal of Instrument ················································...
Page 38 5.3.2 Periodic Maintenance/Checkup ···················································· 5-6 Maintenance/Check Procedure ···························································· 5-7 5.4.1 Checking and Cleaning the Flow Cell ············································· 5-7 Registration of Flow Cell Unit ······························································ 5-11 Replacement Parts and Consumables ·················································· 5-13 Lamp Replacement ············································································ 5-14 5.7.1 Replacement of D Lamp ···························································· 5-15 5.7.2 Replacement of Hg Lamp ····························································...
Page 39: Outline
1. OUTLINE 1.1 Outline of ChromasterUltra R 6430 Diode Array Detector The major features of the ChromasterUltra R 6430 diode array detector are as follows: High Transmittance Flow Cell Based on Total Reflection In order to achieve both of high transmittance of light and small cell volume enough to realize peak resolution for UHPLC applications, silica glass capillary flow cell is employed.
Page 40: Functions
2. FUNCTIONS The functions of the ChromasterUltra R 6430 Diode Array Detector are described below. 2.1 Power Switch and Others on the Front Panel Main switch Door Figure 2-1 Front View  Main (power) switch: Turns power ON and OFF. ...
Page 41: Power Connector And Others On The Rear Panel
2.2 Power Connector and Others on the Rear Panel 2.2 Power Connector and Others on the Rear Panel Power connector USB connector Figure 2-2 Rear View e-Line connector Figure 2-3 Left Side View 2 - 2...
Page 42 Table 2-1 Function of Each Item Item Function Remarks e-Line connector (1) Transmission/reception of (1) Transmission/reception of instrument conditions via e-Line. instrument conditions. (2) Input/output of contact signals: a) START IN a) Program start b) ERROR IN b) Error detected in another instrument c) ERROR OUT c) Error status display...
Page 43: Principle Of Operation
2.3 Principle of Operation 2.3 Principle of Operation lamp Condensing mirror Optical fiber (Incident side) Capillary flow cell Optical fiber Light intlet (Emission side) Light outlet Hg lamp Condensing mirror Photodiode array Changeover mirror Slit Grating Figure 2-4 Optical System Diagram The optical system of ChromasterUltra R 6430 DAD is illustrated in Figure 2-4.
Page 44: Specifications
When carrying out a wavelength accuracy check, the light from lamp is cut off at the changeover mirror, and simultaneously the light from the Hg lamp is reflected into the spectrometer. 2.4 Specifications Optical system: Single beam photometric system (dispersion by diffraction grating) Light source: lamp, Hg lamp (for wavelength check)
Page 45 2.4 Specifications a) Within 0.4  10 (11) Drift: AU/h b) Within 0.4  10 AU/h/C whichever is greater. Measuring conditions using standard flowcell: poured methanol at 0.5 ml/min, COARSE slit, bandwidth 4 nm, smoothed chromatogram 250  10 nm, reference 360 nm  50 nm, sampling period 1600 ms, response 2 s, and after 1 hr warm-up (12) Linearity:...
Page 46 (23) Weight: 17 kg (24) Confidence functions: Used in combination with the Chromato Data Station (CDS) (Maintenance software is included) or the GUI controller. Wavelength accuracy check at 486 mm and 656 mm of D2 lamp emission line, and 254 mm, 365 mm, 436 mm, 546 mm of Hg lamp bright line.
Page 47: System Operation
3. SYSTEM OPERATION Refer to instruction manual for ChromasterUltra R System "Section 3 SYSTEM OPERATION ". 3 - 1...
Page 48: Operation
4. OPERATION The operating procedure for the ChromasterUltra R 6430 Diode Array Detector is explained next. As for the operating procedure for options, refer to the instruction manual provided with each one. CAUTION Fatigue due to long-hour operation If you keep working with the display monitor and keyboard for long hours, your eyes and body will be fatigued to jeopardize your health.
Page 49: Cautions On Operation
4.2 Cautions on Operation 4.2 Cautions on Operation The data acquisition parameters, photometric values, error information etc. of the ChromasterUltra R 6430 Diode Array Detector are all controlled or managed via Chromatography data station (CDS). For the method of using CDS to be installed in the PC, refer to the instruction manual provided with CDS.
Page 50: Selection Of Mobile Phase And Cautions On Handling
4.4 Selection of Mobile Phase and Cautions on Handling Be very careful about the restrictions on use with respect to characteristics and temperature of the mobile phase to be used. (See Table 3-4 Characteristics of Organic Solvents in 3. SYSTEM OPERATION) For high-sensitivity analysis with the diode array detector, use a solvent having a very low UV absorption characteristic.
Page 51 4.4 Selection of Mobile Phase and Cautions on Handling Table 4-1 Reagents for Mobile Phase that may be Restricted Use is Possible If Use is Possible If 10% Reagents Unsuitable Use is Possible 50% or Lower or Lower Phosphoric acid Acetic acid Disodium phosphate Tetrahydrofuran...
Page 52 Concerning the Storage of the Flow cell after use When the flow cell is not used, keep its flow path filled with distilled water or alcohol. When the flow cell is disconnect from the flow path of UHPLC system, connect the loop tube to the outlet of the flow cell and fill the flow path with distilled water or alcohol and connect the other end of the loop tube to the inlet of the flow cell.
Page 53: Maintenance & Inspections
5. MAINTENANCE & INSPECTIONS Confirmation of Basic Actions Install the instrument as instructed in Appendix 1. Turn on the power switch of this detector and make sure LED of "POWER" is on. Make sure the “D LAMP” is lit. Start up CDS, and confirm that the diode array detector is in the “Ready”...
Page 54 5.2 Confirmation of Performance Specifications The performance of diode array detector should be checked with PC (maintenance software of CDS) or GUI (option). For details of performance check method, refer to the respective instruction manuals of maintenance software of CDS, or GUI (option).
Page 55 Measurement of Noise/Drift For measuring noise and drift, refer to the instruction manual for CDS (EZChrom or Empower). Noise and drift need to be measured at wavelengths 250 nm.  Specifications  Noise 0.5  10 AU or less Conditions: Measuring conditions using standard flow cell: poured methanol at 0.5 ml/min, COARSE slit, bandwidth 4 nm,...
Page 56: Periodic Checkup
5.3 Periodic Checkup 5.3 Periodic Checkup 5.3.1 Check of Liquid Leak Sensor For detection of liquid leak, a liquid leak sensor is mounted. However, if the sensor is contaminated, liquid leak might not be detected properly. To prevent this, checkup and cleaning should be carried out periodically.
Page 57 5.3.1 Cleaning of Leak Sensor Hexagonal wrench (P/N: N315003) Remove the leak sensor fastening screw with the furnished hexagonal wrench and separate the leak sensor from the drain tray. Wipe off the water adhering to the leak sensor and drain tray.
Page 58: Periodic Maintenance/Checkup
5.3 Periodic Checkup 5.3.2 Periodic Maintenance/Checkup For ensuring reliable routine operations through prevention of a trouble, periodic checkup must be carried out, referring to Table 5-1. This table lists the items of periodic maintenance/checkup. The interval of periodic checkup is based on the assumption that the instrument operates 8 hours/day and 20 days/month.
Page 59: Maintenance/Check Procedure
5.4.1 5.4 Maintenance/Check Procedure 5.4.1 Checking and Cleaning the Flow Cell WARNING Ignition of flammable chemicals! This instrument is not explosion-proof. During unmanned operation, avoid use of organic solvents having an ignition point below 70 C. Beware of an ignition hazard when using flammable chemicals such as organic solvents.
Page 60 5.4 Maintenance/Check Procedure WARNING Inflammation or injury due to toxic, corrosive or stimulative solvent When using a toxic, corrosive or stimulative solvent, be careful not to incur inflammation or injury. For details of the properties of each solvent and how to handle it, refer to Safety Data Sheet (SDS).
Page 61 5.4.1 If the lamp energy does not recover with the cleaning procedure mentioned (1), clean the light inlet and outlet of the flow cell unit. Loosen two flow cell front lid thumbscrews and detach the flow cell front lid. Thumbscrews Front lid Figure 5-3 Front Cover Clamping Screw Loosen the flow cell clamping screws and separate...
Page 62 5.4 Maintenance/Check Procedure Visually check contamination on both of the light inlet and outlet of flow cell unit. If contamination is found, wipe it with a lens cleaning paper or a cotton swab soaked with methanol. Light inlet Light outlet Figure 5-5 Light Inlet/Outlet of Flow Cell Unit After cleaning the flow cell unit, insert it into the instrument follow the fix blocks.
Page 63: Registration Of Flow Cell Unit
5.5 Registration of Flow Cell Unit When the flow cell unit is replaced with the new one, register the serial number and the code of the new flow cell unit with following procedure. Start the maintenance software of CDS. Open the “Maintenance” screen and select the “DAD” tab. Open the “DAD Meta Parameters”...
Page 64 5.5 Registration of Flow Cell Unit Push the button of the type of the flow cell which is going to be replaced. Input the serial number and the code written on the label attached to the bottom of the new flow cell and register them with Apply button.
Page 65: Replacement Parts And Consumables
5.6 Replacement Parts and Consumables The following table lists the replacement parts and consumables used in this instrument. The majority of these parts (such as lamps) have a lifetime which varies significantly with usage of the instrument. The service life of each part is based on the assumption that the instrument operates 160 hours/month (8 hours/day), 1920 hours/year (240 days).
Page 66: Lamp Replacement
5.7 Lamp Replacement 5.7 Lamp Replacement WARNING Electric shock due to contact with inside of instrument! Before detaching the light source cover for lamp replacement, be sure to turn off the power switch and unplug the power cord from the wall receptacle. WARNING Prohibition of releasing the safety mechanism! In this instrument, the safety mechanism functions to turn...
Page 67: Replacement Of D 2 Lamp
5.7.1 5.7.1 Replacement of D Lamp Part and Tool to be Prepared  New D lamp Check the following.  The power switch of this instrument is turned off.  The power cord is unplugged from the instrument. How to Replace D Lamp Detach the front lid (by removing two thumbscrews).
Page 68 5.7 Lamp Replacement Separate the connector of D lamp and remove two lamp cover fastening screws. And then, detach the lamp cover. lamp cover fastening screw Connector lamp cover Pull out the D lamp from the lamp house. Mount a new D lamp to the lamp house and position it so that the notch on the flange of lamp is in line with the alignment pin on the lamp house.
Page 69 5.7.1 Fasten the D lamp cover to the lamp house by the two fastening screws after passing the lead wire of D lamp through the opening on the center of D lamp cover. Pull the lead wire of D lamp out under the fastening band.
Page 70 5.7 Lamp Replacement Connect the lead wire connector with the connector of the instrument. Pull the lead wire so that the length of the folded wire is as long as possible and fasten it with pulling the fastening band rightward. The fastening screws should be tightened gradually in an alternate order.
Page 71 In this case, ask the maintenance service company authorized by Hitachi High-Technologies for adjustment of the instrument. 5 - 19...
Page 72: Replacement Of Hg Lamp
5.7 Lamp Replacement 5.7.2 Replacement of Hg Lamp Part and Tool to be Prepared  New Hg lamp unit  Phillips screwdriver Check the following.  The power switch of this instrument is turned off.  The power cord is unplugged from the instrument. How to Replace Hg Lamp Detach the front lid (by removing two thumbscrews).
Page 73 5.7.2 Disconnect the lead wire connector of Hg lamp unit from the lamp inverter. Loosen the Hg lamp unit fixing screw and separate the Hg lamp cover. Hg lamp cover Clamp screw Connector 5 - 21...
Page 74 5.7 Lamp Replacement Pull out the Hg lamp unit. Hg lamp unit Hg lamp unit Fit a new Hg lamp unit to the instrument. Attach the Hg lamp cover at the original position and fix the Hg lamp unit with fixing screws. Connect the lead wire connector to the lamp inverter.
Page 75 5.7.2 Operation Check Start the maintenance software. Carry out wavelength check of DAD and make sure a sharp peak (emission line) appears near 253.7 nm in the display of the Hg lamp spectrum acquired with the slit set in Coarse mode. On CDS, reset the cumulative lamp-on time and the lamp-on count.
Page 76: Fuse Replacement
5.8 Fuse Replacement 5.8 Fuse Replacement WARNING Electric shock due to contact with inside of instrument! Before fuse replacement, be sure to turn off the power switch and unplug the power cord from the wall receptacle. Part and Tool to be Prepared ...
Page 77: Troubleshooting
6.1 Troubleshooting For operation and maintenance/checkup of personal computer (PC), printer and HPLC modules, refer to their instruction manuals. Troubleshooting is described here with regard to the ChromasterUltra Rs 6430 Diode Array detector proper. Symptom Cause Judgment/Check Remedy 1. “POWER” LED...
Page 78 (0.25 mm I.D.  1 m Teflon tube or the like) should be added. For details, contact the nearest maintenance service company authorized by Hitachi High- Technologies. (4) Impurities are Check the solvent. Purify or renew the contained in solvent.
Page 79: Error Messages
6.2 Error Messages When the ChromasterUltra Rs 6430 Diode Array Detector is used, error messages appear on the display unit of PC. For error messages at the time of connection, refer to the instruction manual of CDS or maintenance software.
Page 80 Lamp goes out due to liquid Eliminate the cause of liquid message was received from leakage. leakage and wipe off leaked the ChromasterUltra Rs 6430 liquid completely. . Check the Unit Status in the control panel. A "LAMP OFF BY EXTERNAL...
Page 81 If this error message was received from recurs, contact the nearest (2) Failure of circuit board the ChromasterUltra Rs 6430 maintenance service company . Close CDS, restart PC authorized by Hitachi High- and re-connect with unit.
Page 82 6.2 Error Messages Error Message Description Remedy ChromasterUltra Rs 6430 Operation (downloading of Change to the PC Control DAD on process. The instrument method or start of mode with the GUI controller requirement can’t be analysis) of CDS was and make a retry.
Page 83: Accessories
7. ACCESSORIES 7.1 Flow Cell Name Items  Standard flow cell 889-0810 Standard flow cell  Loop tube  Light inlet/outlet caps (2 pcs)  High sensitivity flow 889-0811 High sensitivity flow cell cell  Loop tube (option)  Light inlet/outlet caps (2 pcs) NOTICE: The transmittance of quartz currently used for the optical penetration part of a flow cell falls down to...
Page 84: Index
8. INDEX Cautions on operation ......................4-2 Check of lamp energy ....................... 5-19 Check of leak sensor ......................5-4 Checking and cleaning the flow cell ..................5-7 Cleaning of leak sensor ...................... 5-5 Confirmation of performance specifications ................ 5-1 Connectors ...................... APPENDIX 2-2 Error message ........................
Page 85 8. INDEX Rear panel .......................... 2-2 Registration of flow cell unit ....................5-11 Replacement of D lamp ....................5-15 Replacement of Hg lamp ....................5-20 Replacement parts and consumables ................5-13 Shutdown ........................... 4-2 Specifications ........................2-5 Troubleshooting ........................6-1 Washing ..........................
Page 86: Appendix
APPENDIX APPENDIX 1 INSTALLATION CAUTION Caution in Carrying Heavy Instrument ChromasterUltra R Organizer weighs as much as 9 kg. Be careful not to drop it and to handle it with care when carrying it, for preventing injury. Hold the right and left parts of the instrument firmly.
Page 87: Unpacking
If relocation of the instrument becomes necessary after delivery, consult your dealer or nearest Hitachi High-Tech Science service representative in order to avoid possible trouble involved in relocation. APPENDIX 1 - 2...
Page 88: Place For Installation
2. Place for Installation For installation of the instrument, select a place which meets the following conditions. Operating temperature: Within 15 to 35 C and with minimum temperature variation (2 °C/h) during measurement. Utmost care should be used for preventing condensation. Operating humidity: 25 to 85% Atmospheric gas...
Page 89: Removal Of Transport Fixed Plate (Chromasterultra R 6270 Autosampler)
(h) Power supply to motor-driven equipment (such as stirrer and vibrator) without a noise suppressor shall not be turned ON/OFF frequently on the same power supply line as that of this instrument. 2.1 Removal of Transport Fixed Plate (ChromasterUltra R 6270 Autosampler) Open the front door of ChromasterUltra R 6270...
Page 90: Removal Of Transport Screws (Chromasterultra R 6430 Dad)
2.2 Removal of Transport Screws (ChromasterUltra R 6430 DAD) Remove Transport Screws from ChromasterUltra R 6430 Diode Array Detector. There are two screws for transport on the bottom of the detector. Remove them using a Phillips screwdriver and attach the washers with the accessory short screws at that place.
Page 91 2.3 Removal of Transport Screws (ChromasterUltra R 6440 FL Detector) Remove Transport Screws from ChromasterUltra R 6440 Fluorescence Detector. There are three screws for transport on the bottom of the detector. Remove them (screw for transport 1, 2, 3) using a Phillips screwdriver Screws for transport 3 (for fixing lamp house)
Page 92 Detach a lamp house from the detector (refer to section 5.6.1) Anode nut Lamp house New Xenon lamp Cathode nut Appended Fig. 1-4 Attaching Xenon Lamp Attach a Xenon lamp to the lamp house (refer to section 5.6.1). Adjust the position of xenon lamp (refer to section 5.6.1) NOTE: Please keep the transport screw after detaching.
Page 93 2.4 Removal of Transport Screws(ChromasterUltra R 6420 UV-VIS Detector) Remove grommets, please remove two screws for transport. Please attach two screws provided in screw holes. Please attach grommets to the original position. Grommets, Two Screws for tansport The screw on the optical base fixing Appended Fig.
Page 94: Items To Be Prepared By Customer
3. Items to be Prepared by Customer 3.1 Power Supply Arrangement Power supply voltage: 100 to 240 V AC Fluctuation shall be ±10% of the rated voltage. Frequency: 50 or 60 Hz Fluctuation shall be ±0.5 Hz of the rated frequency. Power Capacity: 1100 VA or more The total system shown comprises ChromasterUltra R...
Page 95 WARNING Electric shock due to improper grounding! This instrument is designed to meet the basic insulation stipulated in the international standard IE C61010-1. To prevent an electric shock hazard due to improper grounding, provide a proper grounding connection. Be sure to use the grounded 3P power cable, which is supplied as a standard accessory for the instrument.
Page 96 (a) Usual Grounding Connection (with grounded 3P power outlet) Grounded 3P power outlet (15 A) Grounded 3P table tap Power cord (attached to ChromasterUltra R instrument) 6170 Binary Pump 750 mm or Ground resistance 100  or less more below ground Connector Rear panel...
Page 97 (b) Connection when Grounded Power Outlet is Unavailable 2P power outlet (15 A) 3P table tap with grounding wire Power cord (attached to ChromasterUltra Rs Grounding instrument) 6170 Binary Pump terminal 750 mm or Ground more below resistance ground Connector 100 ...
Page 98: Installation Space
3.2 Installation Space For composing a liquid chromatograph system with the ChromasterUltra R System, a space of 735 mm wide and 765 mm deep is required at least. Behind each unit, a space of 150 mm or more must be secured. At the left and right sides of the respective units, a space of minimum 100 mm each is necessary.
Page 99: Assembling
5. Assembling NOTE: It is not permitted for the user to carry out installation at the time of delivery. For ensuring safety and high accuracy in operation of the instrument, installation of the instrument shall be carried out by or under supervision of qualified service personnel of the manufacturer or its authorized service agent.
Page 100: System Power Supply
5.1 System Power Supply For ChromasterUltra R System, the AC power cables are connected to each unit. Plug the power cables into AC power outlets on back side of instruments. ChromasterUltra R Organizer Inter Face Control Board ChromasterUltra R 6420 UV-VIS ChromasterUltra R 6430 DAD ChromasterUltra R...
Page 101: System Cabling
5.2 System Cabling In the ChromasterUltra R System, the module units are cabled by plugging the e-Line cable into the e-Line connector at the rear left of each unit. Rear panel side e-Line connector Appended Fig. 1-10 e-Line Cable Connector e-Line Cable Connection ChromasterUltra R Organizer...
Page 102 ChromasterUltra R Organizer Inter Face Control Board ChromasterUltra R 6420 UV-VIS ChromasterUltra R 6430 DAD ChromasterUltra R ChromasterUltra R 6310 Column Oven 6270 Autosampler ② ChromasterUltra R ChromasterUltra R 6170 Binary Pump 6440 FL Detector Appended Fig. 1-12 e-Line Cable Connection （with CM6440 FL Detector）...
Page 103 ChromasterUltra R Organizer Inter Face Control Board ChromasterUltra R 6420 UV-VIS ChromasterUltra R 6430 DAD ChromasterUltra R 6310 Column Oven ChromasterUltra R 6270 Autosampler ChromasterUltra R 6440 FL Detector ChromasterUltra R 6170 Binary Pump Appended Fig. 1-13 e-Line Cable Connection（with CM6440 FL Detector） (The FL detector is connected to a DAD (or UV-VIS detector) in parallel.) Appended Table 1-2 Details of Cable e-Line cable 1) (50 cm)
Page 104 USB Cable Connection ChromasterUltra R Organizer Inter Face Control Board ChromasterUltra R 6420 UV-VIS ChromasterUltra R 6430 DAD ChromasterUltra R ChromasterUltra R 6310 Column Oven 6270 Autosampler ChromasterUltra R ChromasterUltra R 6170 Binary Pump 6440 FL Detector To the USB connector of the PC Appended Fig.
Page 105 Appended Fig. 1-15 Driver Installation Screen, which is wrong APPENDIX 1 - 20...
Page 106: System Tubing
5.3 System Tubing In the ChromasterUltra R System, the inlet tube up to the pump is fastened to the left side face of each unit with tube clamps. ChromasterUltra R DAD System Flexible tube 400 Drain tube D Inlet tube A1 Inlet tube A2 Inlet tube B1 Inlet tube B2...
Page 107 Connection to tube 1. 6170 Binary Pump Connection to double corkscrew mixer Flexible tube 300 2. 6270 Autosampler Connection to injection valve (a) Flexible tube 300 (b) Flexible tube 400 3. 6310 Column Oven Connection to preheat plate (b) Flexible tube 400 (c) Flexible tube 180 or MEM column fitting 4.
Page 108 ChromasterUltra R UV-VIS System Flexible tube 400 Drain tube Inlet tube A1 Inlet tube A2 Inlet tube B1 Inlet tube B2 Flexible tube 180 Inlet tube AS1 Inlet tube AS2 Inlet tube W Flexible tube 400 WP tube Drain tube P Flexible tube 300 Appended Fig.
Page 109 Drain Tubing (Right side view) (Front side view) T-joint T-joint Drain tube 1 Drain tube 1 T-joint T-joint Drain tube 2 Drain tube 2 Waste fluid tank P-OV tray Drain tube 3 Appended Fig. 1-18 Tubing Diagram for Drain Appended Table 1-6 Drain Tubes to be Used T-joint P/N 810-1362 (attached to autosampler)
Page 110 ChromasterUltra R Two Detectors System (DAD or UV-VIS detector + FL detector) The FL detector is connected to a DAD (or UV-VIS detector) in series. Flexible tube 400 Inlet tube A1 Inlet tube A2 Flexible tube 180 Inlet tube B1 Inlet tube B2 Inlet tube AS1 Inlet tube AS2...
Page 111 a) As for the analysis result of FL detector installed in the downstream side, a peak may become broad by the increase in system volume. b) Please be careful of the resisting pressure of the flow cell of DAD or UV-VIS detector installed in the upper stream side.
Page 112 ChromasterUltra R Two Detectors System (DAD or UV-VIS detector + FL detector) The FL detector is connected to a DAD (or UV-VIS detector) in parallel. Inlet tube A1 Flexible tube 400 Inlet tube A2 Inlet tube B1 Flexible tube 180 Inlet tube B2 INLET semi-micro U assy Inlet tube AS1...
Page 113: Fastening Between Component Units
5.4 Fastening between Component Units CAUTION Prevention of Instrument Tipover Personal injury could be incurred if the instrument tips over. When installing the instrument, provide a proper means for preventing the instrument from tipping over accidentally. On the rear of each module unit, a cabling cover is attached. The adjacent models are fastened with each other by attaching the fastening plate between the wiring covers.
Page 114: Communication
APPENDIX 2 DESCRIPTION ON CONTACT SIGNAL COMMUNICATION 1. Outline The following two types of contact signals are used in the ChromasterUltra R series. Contact signals (START, ERROR, BUSY) entering the e- Line connector. These are contact signals having input/output functions that are used in common for each unit.
Page 115 Connection with L-2000, Chromaster S Series Use 3-pin connectors of the e-DIO cab ble (890-6145) in the connection shown below. ERROR START STOP D-Lin conn nector To L-2000, To ChromasterUltra R Chromaster series instrument series instrument e-Line connector BUSY 3-pin connecto e-DIO cable e-DIO cable (890-6145)
Page 116: Individual Contacts Of Each Unit
3. Individual Contacts of Each Unit Contact Signal Input Terminals These are contact signal input termina ls for control using contact signals from an external switch, relay etc. Any of the signals is activated by shorting the term minal for 0.1 second or longer.
Page 117 +5 V  Max. 10 mA Chromaster series u unit External unit Activated u upon shorting contacts of external unit. Append ded Fig. 2-3 Contact Signal Input Circuit 0.1 A max. 30 V max. Chromaster series u unit External unit Append ded Fig.
Page 118: Appendix 3 Terminologies
APPENDIX 3 TERMINOLOGIES e-line Used for an analysis-specific digital network. Interface control board to control the ChromasterUltra R series instrument by PC. An interface control board (IFC board) is a standard equipment of the ChromasterUltra R Organizer. Liquid leak sensor A sensor to detect liquid leakage.
Page 119 Peltier A semiconductor device that allows heat exchange from one direction to another by flowing a current. Cooling or heating is possible according to the direction of current flow. Sample rack A component part on which a sample is set. Septum Sheet used as the lid for a vial.
Page 120 Auto zero Electrically adjusts absorbance to zero, thereby canceling a rise of the baseline. Generally activated just before or after start. Grating An optical device for dispersing incident beam according to differences in wavelength. Output range Determines the amplitude of a signal to be output to an external system such as data processor.
Page 121 Flow cell A sample container for measuring a sample continuously. Lamp house A section where the light source lamp is accommodated. The lamp gets hot when the instrument is used. Be careful not to get burned. Sample-side energy Refers to the intensity of beam that passes through a sample in a flow cell.

Hitachi ChromasterUltra RS 6430 Instruction Manual

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