GE Frac-920 Operating Instructions Manual
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GE Frac-920 Operating Instructions Manual

Fraction collector
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Fraction Collector Frac-920 and
Frac-950
Operating Instructions
Original instructions
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Summary of Contents for GE Frac-920

  • Page 1 Fraction Collector Frac-920 and Frac-950 Operating Instructions Original instructions...

  • Page 2: Table Of Contents

    Unpacking ..............................Assembly ..............................Connections ............................Spare parts and accessories ......................Operation ......................Fraction Collector Frac-920 stand alone ................... Fraction Collector Frac-920 connected to an ÄKTA instrument ........Fraction Collector Frac-950 ......................Maintenance ......................General ..............................Cleaning ..............................Changing capillaries ..........................

  • Page 3: Introduction

    The Operating Instructions provide you with the instructions needed to handle Fraction Collector Frac-920 and Frac-950 in a safe way. Prerequisites In order to operate Fraction Collector Frac-920 and Frac-950 as is intended, the following pre-requisites must be fulfilled: ®...

  • Page 4: Important User Information

    ÄKTA™ chromatography systems. Fraction Collector Frac-920 can also be used as a stand-alone unit. Fraction Collector Frac-920 and Frac-950 are intended for research use only, and shall not be used in any clinical procedures, or for diagnostic purposes.

  • Page 5: Regulatory Information

    Hardware items are identified in the text by bold text (e.g., Power switch). Regulatory information In this section This section describes the directives and standards that are fulfilled by Fraction Collector Frac-920 and Frac-950. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 6 • connected to other products recommended or described in the user documentation, • used in the same state as it was delivered from GE Healthcare, except for alterations described in the user documentation. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 7 The user is cautioned that any changes or modifications not expressly approved by GE Healthcare could void the user’s authority to operate the equipment. This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to part 15 of the FCC Rules.

  • Page 8: Instrument

    Registration, Evaluation, Authorization and restriction of No 1907/2006 CHemicals (REACH) Instrument Fraction Collector Frac-920 and Frac-950 are automated fraction collectors for use in ÄKTA chromatography systems. Fraction Collector Frac-920 can also be used as a stand- alone unit. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...

  • Page 9: Fraction Collector Frac-920

    1 Introduction 1.3 Instrument 1.3.1 Fraction Collector Frac-920 1.3.1 Fraction Collector Frac-920 Main parts Table 1.1: The main parts of Fraction Collector Frac-920. Part Description Delivery arm Lock knob Control buttons Control buttons Collection tubes Tube rack Programming buttons Menu display...
  • Page 10 1 Introduction 1.3 Instrument 1.3.1 Fraction Collector Frac-920 Electrical and communication connections Part Description Drop sensor Valve Auxillary equipment Remote/digital I/O UniNet-1 Power inlet Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...

  • Page 11: Fraction Collector Frac-950

    1 Introduction 1.3 Instrument 1.3.2 Fraction Collector Frac-950 1.3.2 Fraction Collector Frac-950 Main parts Table 1.2: The main part of Fraction Collector Frac-950 Part Description Delivery arm Safety bar Collection tubes Tube rack Indicators Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...

  • Page 12: Control Software

    Control software UNICORN control software ™ UNICORN is a complete software for control and supervision of Fraction Collector Frac-920 ® and Frac-950. The software runs under Microsoft Windows operating system. For more information about UNICORN control system, see the UNICORN user manuals supplied.
  • Page 13 Software documentation Together with each system, the following software documentation is supplied providing additional information that applies to Fraction Collector Frac-920 and Frac-950, indepen- dent of the specific configuration: Document Purpose/Contents UNICORN™ manual package The manuals contain detailed instructions on •...

  • Page 14: Safety Instructions

    Safety precautions Introduction Fraction Collector Frac-920 and Frac-950 are powered by mains voltage and handles liquids that may be hazardous. Before installing, operating or maintaining the system, you must be aware of the hazards described in this manual. Follow the instructions provided to avoid personal injury or damage to the equipment.
  • Page 15 Do not use any accessories not supplied or recommended by GE Healthcare. WARNING Do not use the Fraction Collector Frac-920 and Frac-950 if it is not working properly, nor if it has suffered any damage, for example: • damage to the power cord or its plug •...
  • Page 16 Personal protection WARNING Always use appropriate personal protective equipment during operation and maintenance of Fraction Collector Frac-920 and Frac-950. WARNING When using hazardous chemical and biological agents, take all suitable protective measures, such as wearing protective glasses and gloves resistant to the substances used.
  • Page 17 WARNING Fraction Collector Frac-920 and Frac-950 must always be connect- ed to a grounded power outlet. WARNING Power cord. Only use power cords with approved plugs delivered or approved by GE Healthcare.
  • Page 18 Be sure to fold down the safety bar whenever the rack holder is operated by hand. This blocks the rack holder from accidentally moving while the rack is moved by hand or replaced. (Fraction Collector Frac-950 only) Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...

  • Page 19: Labels

    In this section This section describes the instrument labels and labels concerning hazardous substances that are attached to the Fraction Collector Frac-920 and Frac-950 instrument. For infor- mation about marking of the computer equipment, refer to the manufacturer’s instruc- tions.
  • Page 20 (EMC) in Australia and New Zealand. The equipment complies with applicable European directives. This symbol indicates that Fraction Collector Frac-920 and Frac-950 has been certified by a Nationally Recognized Testing Laboratory (NRTL). NRTL means an organization, which is recognized by the US...

  • Page 21: Emergency Procedures

    Emergency procedures In this section This section describes how to do an emergency shutdown of the Fraction Collector Frac-920 and Frac-950 system. The section also describes the result in the event of power failure. Emergency shutdown In an emergency situation, do as follows to stop the run:...

  • Page 22: Recycling Information

    Disposal, general instructions When taking Fraction Collector Frac-920 and Frac-950 out of service, the different ma- terials must be separated and recycled according to national and local environmental regulations.

  • Page 23: Declaration Of Hazardous Substances (Dohs)

    EFUP shall be done in accordance with the Product Maintenance Procedures. This product must not be disposed of as unsorted municipal waste, and must be collected separately and handled properly after decommissioning. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 24 Indicates that this toxic or hazardous substance contained in at least one of the homoge- neous materials used for this part is above the limit requirement in SJ/T11363-2006. Data listed in the table represents best information available at the time of publication. • Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...

  • Page 25: Installation

    3 Installation Installation Fraction Collector Frac-920 and Frac-950 is delivered in protective packing material and shall be unpacked with great care. Any equipment connected to Fraction Collector Frac-920 and Frac-950 must fulfill appli- cable standards and local regulations. For detailed information on Installation, see Fraction Collector Frac-920 and Frac-950 User Manuals.

  • Page 26: Unpacking

    Unpack the system Remove straps and packing material. Then set the equipment upright before starting installation. Assembly The following parts must be added to the Fraction Collector Frac-920 and Frac-950 in- strument before it can be used: • Tube rack •...

  • Page 27: Spare Parts And Accessories

    3 Installation 3.5 Connections Flow path Connect inlet and outlet tubing to the system. See Fraction Collector Frac-920 and Frac-950 User Manuals. Electrical power Connect the power cord to a grounded power outlet specified in Section 3.1 Site require- ments, on page...

  • Page 28: Operation

    4 Operation Operation About this chapter This chapter provides instructions for the use of Fraction Collector Frac-920 and Frac-950. Fraction Collector Frac-920 stand alone Starting the instrument 1 Connect the power cord to the mains power outlet. The display lights up and the system immediately performs a self-test.
  • Page 29 4 Operation 4.1 Fraction Collector Frac-920 stand alone The Fraction Collector Frac-920 software is divided into three main menus: Menu Description Run Method is used for running methods that are pro- grammed by the user. The Run Method menu is dis- played after the self-test.
  • Page 30 Press pause/cont. to pause all operation without ending the method. All functions, including pump and fraction collector, are stopped. Press pause/cont. again to restart the method operation. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 31 Note: Before starting to create a method the method base should be checked (for more information, refer to Fraction Collector Frac-920 User Manual). Creating a new method To create a new method: From the Run Method menu, press to reach the Program Method menu.
  • Page 32 When a new method is created or a current is edited, the first displayed menu will be Set Fraction Size. 1 Press OK and select a fraction size using the arrow buttons. 2 Press OK. To set fraction start, press . Press OK. 4 Set a start value. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 33 2 Select Peak Detect: Level or Slope. Press OK. • If Level has been selected, set an absorbance level (% FSD) when to start collecting fractions. • If Slope has been selected, set a slope (%/min). • Press OK. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 34 4 Press OK. For more information about the peak detection parameters, refer to Fraction Collector Frac-920 User Manual. Saving the method 1 Decide to save the method, select yes or no. 2 Press OK. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 35 Used for running methods that are programmed by the user. Description Description Run menu 1 Run menu 2 1 Use the Esc and arrow buttons to reach the Run Method menu. 2 Press OK. 3 Select which method to run. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 36 Method run length Tube no. Actual tube volume/fraction or peak fraction size. Tube volume is counted from 0 to set value. Run menu 2 Description Flow (ml/min), if a valid pump is connected. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...

  • Page 37: Fraction Collector Frac-920 Connected To An Äkta Instrument

    It is also possible to set the delay volume, which corresponds to the volume between the UV monitor and Fraction Collector Frac-920. This value must be changed if the tubing is changed to tubing of a different inner diameter, see...
  • Page 38 3 The self-test takes about five seconds. When the self-test is completed, the display shows the Run Method menu. 4 When UNICORN connects to Fraction Collector Frac-920, the displays shows the message Uninet 1 connected for for a few seconds.

  • Page 39: Fraction Collector Frac-950

    4.2 Fraction Collector Frac-920 connected to an ÄKTA instrument Setting the delay volume The delay volume between the UV monitor and Fraction Collector Frac-920 in your ÄKTA chromatography system can be set. The fraction marks shown in UNICORN will be ad- justed according to this volume to show the actual parts collected.
  • Page 40 The following functions are available for operating Fraction Collector Frac-950 from UNICORN: Manual Method Man_Fractionation Fractionation Fractionation_Stop Fractionation_Stop Feed_Tube Feed_Tube Man_Peak_Fractionation Peak_Fractionation Peak_Frac_Stop Peak_Frac_Stop AccumulatorWash AccumulatorWash Ignore_LastTube Reset_Frac_Position Reset_Frac_Position (PeakFrac Parameters (PeakFrac Parameters) Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 41 ÄKTA design Optional Configuration User Manual. Collecting peak fractions Details about collecting peaks only using Fraction Collector Frac-950 in a method are described in the ÄKTA design Optional Configuration User Manual. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 42 You can then fill up with new tubes, and use the instruc- tion Ignore_LastTube. 1 Select menu System Control:Manual:Frac in UNICORN. 2 Select the instruction Ignore_LastTube in the Frac list. 3 Click on the Execute button. 4 Fill up the rack with new tubes. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 43 Outlet Valve to the other port. Select menu System Con- trol:Manual:Flowpath in UNICORN. 3 Select the instruction OutletValve and select the desired port. 4 Click on the Execute button. 5 Select menu System Control:Manual:Frac in UNICORN. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 44 4 The appropriate value for your ÄKTA system is found in the ÄKTA design Optional Configuration User Manual. 5 Click on the OK button. The value entered will be used until a further change is made. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 45 For the 30 mm rack and Prep mode racks the accumulator is recom- mended. Rack type Flow rate limit using dropSync [ml/min] microplates 0 to 1.0 12 mm 0 to 1.5 18 and 30 mm 0 to 2.0 Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 46 Define rack and tube parameters When running a method, the rack and tube parameters for the rack to be used must be set. This is described in detail in the ÄKTA design Optional Configuration User Manual. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...

  • Page 47: Maintenance

    5 Maintenance Maintenance General Fraction Collector Frac-920 and Frac-950 require no periodic maintenance. Cleaning WARNING When using hazardous chemical and biological agents, take all suitable protective measures, such as wearing protective glasses and gloves resistant to the substances used. Follow local and/or national regulations for safe operation and maintenance of Fraction Collector Frac-920 and Frac-950.

  • Page 48: Changing Capillaries

    5.3 Changing capillaries Changing capillaries Change the capillaries when they show signs of leakage or wear (sharp bending, for example). Changing waste tubing Change the waste tubing when it shows signs of wear. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...

  • Page 49: Troubleshooting

    This blocks the rack holder from accidentally moving while the rack is moved by hand or replaced. (Fraction Collector Frac-950 only) CAUTION Only spare parts that are approved or supplied by GE Healthcare may be used for maintaining or servicing the unit. Faults and actions Fault...
  • Page 50 Check that the rack is correctly fitted to the rack holder. • Check that the capillary end is cut cleanly and straight. • Check that the correct rack type is selected. • Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...

  • Page 51: Reference Information

    Power consumption 300 VA (Frac-950) 20 VA (Frac-920) Dimensions (H × W × D) 480 × 380 × 550 mm (Frac-950) 250 × 320 × 400 mm (Frac-920) Weight 16.5 kg (Frac-950) 4.5 kg (Frac-920) Ambient temperature 4° to 40°C...

  • Page 52: Health And Safety Declaration Form

    Bio-Sciences Co rp, 800 Centennial A Avenue, P.O. Box 132 7, Piscataway, NJ 08855-1 1327 © 2010-14 General Electric Com mpany—All rights res served. First publishe ed April 2010. DOC1149542/28-980 00-26 AC 05/2014 Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 53 NJ 08855-1 1327, US © 2010-14 General Electric Com mpany—All rights res served. First publishe ed April 2010. DOC1149544/28-980 00-27 AC 05/2014 Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...

  • Page 54: Ordering Information

    7 Reference information 7.3 Ordering information Ordering information For ordering information visit www.gelifesciences.com/AKTA. Frac-920 and Frac-950 Operating Instructions 28-9622-09 AC...
  • Page 55 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare repre- sentative for the most current information.

This manual is also suitable for:

Frac-950

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