Page 1 INSTRUCTION MANUAL 889-9121-009 Ver.10 June. 2017 Ultra High Performance Liquid Chromatograph ChromasterUltra R 6270 AUTOSAMPLER...
Page 2 Hitachi High-Tech Science Corporation. 3. Hitachi High-Tech Science Corporation assumes no liability for any direct, indirect, or consequential damages arising from use not described in this manual.
Page 3: Preface
PREFACE Thank you very much for purchasing the ChromasterUltra Rs System. The ChromasterUltra Rs System is designed to perform liquid chromatography which separates and detects the existence in a sample. Note that physiologically or biologically active samples are not applicable to the ChromasterUltra Rs System because of possible infection with bacteria or viruses.
Page 4: About This Manual
ABOUT THIS MANUAL This instruction manual describes how to use and maintain the ChromasterUltra Rs System. This instruction manual consists of the following sections. IMPORTANT (Warranty, Installation, Relocation, After-sale Technical Service, etc) SAFETY SUMMARY (collected and summarized) Section 1 OUTLINE Section 2 FUNCTIONS Section 3...
Page 5: Important
IMPORTANT Warranty on Product Limited Warranty The ChromasterUltra R System is warranted to be free from defects in material or workmanship under normal use within the product specifications indicated in this manual and under conditions given below. This warranty is void if the software is not used according to the instruction manual.
Page 6 Warranty Limitations and Exclusions Disclaimer of Warranty THE MANUFACTURER MAKES NO WARRANTIES, EITHER EXPRESS OR IMPLIED, EXCEPT AS PROVIDED HEREIN, INCLUDING WITHOUT LIMITATION THEREOF, WARRANTIES AS TO MARKETABILITY, MERCHANTABILITY, FOR A PARTICULAR PURPOSE OR USE, OR AGAINST INFRINGEMENT OF ANY PATENT. IN NO EVENT SHALL THE MANUFACTURER BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT.
Page 7: Installation, Relocation And After-Sale Technical Service
(m) Failure due to a life-limited part that has exceeded the end of its useful lifetime. Limitations of Liability Hitachi High-Tech Science shall not be liable in contract or in tort (including, without limitation, negligence, strict liability or otherwise) for claims by third parties (other then for bodily injury or...
Page 8: Technical Seminars And Training Courses For Users
After-sales Service (a) For after-sale technical service of this instrument, please notify our local sales representative or service office. (b) A maintenance & service contract is available for servicing the instrument after the warranty period has ended (service available at charge). (c) Maintenance parts and consumables will be supplied during the useful life of this instrument (7 years).
Page 9: Caution On Disposal Of Instrument
Note, however, that the environmental protection laws and regulations may be revised or amended. Therefore, be sure to consult with your local Hitachi High-Technologies sales or service representative when planning disposal of this instrument or its parts and accessories.
Page 10: Information For Users On Weee (Only For Eu Countries)
Information for Users on WEEE (only for EU Countries) This symbol is in compliance with the Waste Electrical and Electronic Equipment directive 2012/19/EU (WEEE). This symbol on the product indicates the requirement NOT to dispose of the equipment as unsorted municipal waste, but use the return and collection systems available.
Page 11 Disturbance by Electromagnetic Wave This instrument conforms to Class A in the EN standards EN61326-1 (2013). Avoid installing this instrument near equipment whose data will be affected by electromagnetic noise within the permissible range of this standard. Also, data of this instrument may be affected by electromagnetic noise or the instrument itself may malfunction.
Page 12: Safety Summary
Do not perform any operation or action other than described in these manuals.  On occurrence of any trouble in the instrument, notify the nearest sales service representative of Hitachi High-Technologies.  Keep in mind that the hazard warnings in the manuals or on the product cannot cover every possible case, as it is impossible to predict and evaluate all circumstances beforehand.
Page 13: General Safety Precautions
Disconnect the power cord plug from the power outlet. Then, after providing proper safety measures as required, contact the nearest service representative of Hitachi High-Technologies. Using the instrument in such an abnormal condition could result in an electric shock or fire.
Page 14: Warning Precautions In The Manual
SAFETY SUMMARY WARNING precautions in the Manual WARNING Ignition of Flammable Chemicals  This instrument is not explosion-proof. In unattended operation, do not use organic solvents having an ignition point below 70 C (Manual System Section 3) (Manual Binary Pump Section 4) (Manual Autosampler Section 5) (Manual Column Oven Section 2) (Manual UV-VIS Section 5) (Manual DAD Section 5) (Manual Organizer Section 3) (Manual FL Section 5)
Page 15 SAFETY SUMMARY WARNING precautions in the Manual (Continued) WARNING Explosion of Vapor from Flammable Chemicals If a flammable chemical such as organic solvent leaks from the flow path of the instrument and its vapor concentration reaches the explosion limit, it could cause spontaneous combustion with dangerously explosive results.
Page 16 SAFETY SUMMARY WARNING precautions in the Manual (Continued) WARNING Electric Shock due to Improper Grounding This instrument is designed in conformity with the specifications of Class I in Annex H of the IEC 61010-1 (International Electrotechnical Commission Standards) – Issue 1. To prevent an electric shock hazard, provide a proper grounding connection.
Page 17 SAFETY SUMMARY WARNING precautions in the Manual (Continued) WARNING Injury of Hand by Needle or Internal Mechanism Never touch the inside of the autosampler during operation, as your hand or finger may be seriously injured by the needle or internal mechanism. (Manual Autosampler Section 4.2) Prohibition of Releasing the Safety Mechanism In this instrument, the safety mechanism functions to turn off power supply automatically...
Page 18 SAFETY SUMMARY WARNING precautions in the Manual (Continued) WARNING Injury due to Xenon Lamp Explosion [In use]  When the cumulative turn-on time of the xenon lamp exceeds the guaranteed useful lifetime, the electrodes evaporate and the scattered matter sticks to the bulb wall, so its blackening progresses and heat dissipation is hindered.
Page 19 SAFETY SUMMARY WARNING precautions in the Manual (Continued) WARNING Injury due to Xenon Lamp Explosion [At mounting] (Continued)  Do not touch the quartz glass part of the xenon lamp with bare hands. If the quartz glass part of the xenon lamp is contaminated with dust or fingerprints, wipe it using a gauze sheet or absorbent cotton cloth slightly moistened with high-quality alcohol.
Page 20: Caution Precautions In The Manual
SAFETY SUMMARY CAUTION Precautions in the Manual CAUTION Caution in Carrying Heavy Instrument  ChromasterUltra R Organizer weighs as much as 9 kg. Be careful not to drop it and to handle it with care when carrying it, for preventing injury. Hold the right and left parts of the instrument firmly.
Page 21 SAFETY SUMMARY CAUTION Precautions in the Manual (Continued) CAUTION Prevention of Instrument Tipover Personal injury could be incurred if the instrument tips over. When installing the instrument, provide a proper means for preventing the instrument from tipping over accidentally. (APPENDIX 1) Fatigue due to Long-Hour Operation If you keep working with the display monitor and keyboard for long hours, your eyes and body will be fatigued to jeopardize your health.
Page 22 SAFETY SUMMARY CAUTION Precautions in the Manual (Continued) CAUTION  The light source lamp and its cover become hot during operation and remain hot for a while even after power-off. They can severely burn you if touched. Before replacement of the lamp, turn power off and wait for about 60 minutes until it cools down sufficiently.
Page 23 If any one of the warning labels becomes s illegible due to deterioration, contact your lo ocal Hitachi High-Technologies Corporat tion sales representative or service office of H Hitachi High-Technologies Corporat tion sales representative for replacement with h a new one.
Page 24 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument ChromasterUltra R 6170 Bi inary Pump (1) Ignition of Fla ammable Chemicals (2) Explosion of Vapor from Flammable Chemicals Left sid de view (3) Electric Shoc ck due to Contact with Inside o of Instrument Rear Pan...
Page 25 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument ChromasterUltra R 6270 Au utosampler (1) Ignition of Flamm mable Chemicals (2) Explosion of Vap por from Flammable Chemicals Left side v view (3) Electric Shock d due to Contact with Inside of Ins strument Rear Pan...
Page 26 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument (4) Damage to Needle by Misss setting Sample Rack Inside View (L Left Side) (5) Injury of Hand by Needle or r Internal Mechanism Front Vi SAFETY - 15...
Page 27 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument ChromasterUltra R 6310 Co olumn Oven (1) Electric Shoc k due to Contact with Inside of f Instrument Rear Panel SAFETY - 16...
Page 28 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument (3) Do Not Touch H Hot Part (2) Ignition of Flammable Che emicals (4) Explosion of Va apor from Flammable Chemicals Right side e view SAFETY - 17...
Page 29 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument ChromasterUltra R 6420 UV V-VIS detector (1) Ignition of Flammable e Chemicals (2) Explosion of Vapor from F Flammable Chemicals Left side view (3) Electric Shock due to Contact t with Inside of Instrument ar Panel SAFETY - 18...
Page 30 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument (4) Electric Shock due to Cont tact with Inside of Instrument (5) Do Not Touc h Hot Part Front View SAFETY - 19...
Page 31 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument ChromasterUltra R 6430 Di iode Array detector (1) Ignition of Flammable e Chemicals (2) Explosion of Vapor from m Flammable Chemicals Left side view (3) Electric Shock due to Contact with Inside of Instrument ar Panel SAFETY - 20...
Page 32 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument (5) Burns due to Contact w ith Hot Parts (4) Electric Shock due to Con ntact with Power Supply to Light Source Front View SAFETY - 21...
Page 33 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument ChromasterUltra R 6440 FL L detector (1) Explosion of Vapor fro om Flammable (2) Electric Shock due to Co ontact with Light Chemicals Source Power Supply Front View (3) Ignition of Flammable Chemic cals (4) Burns due to Contact with Hot Parts Explosion of Xenon...
Page 34 AFETY SUMM MARY WARNING a nd CAUTION Labels on In nstrument (6) Elect ric Shock due to Conta act with Inside of Instru ument Rear Panel SAFETY - 23...
Page 35: Notice Precautions
SAFETY SUMMARY NOTICE precautions Precautions on Use of Solvents  Alumina, EPDM (ethylene-propylene rubber), ETFE (Ethylene tetrafluoroethylene), FEP (fluorinated ethylene-propylene), FFPM (Perfluoroelastomer), PCTFE (polychlorotrifluoroethylene), PEEK (polyether ether ketone), PP (polypropylene), PPS (Polyphenylene sulfide), PTFE (polytetrafluoroethylene), PVDF (polyvinylidene fluoride), Quartz glass, Ruby, Sapphire, SUS316 (Stainless steel), Teflon®AF, UHMWPE (ultra high molecular weight polyethylene), Vespel®...
Page 36 SAFETY SUMMARY NOTICE precautions (Continued)  Referring to the instruction manual about each module unit of the liquid chromatograph system, set up the automatic liquid feed stop function (such as the pressure limiter function of the pump unit) to prevent a possible trouble. For detailed information, refer to the instruction manual about the ChromasterUltra R 6170 Binary Pump.
Page 37: Table Of Contents
CONTENTS PREFACE ············································································································ 1 ABOUT THIS MANUAL ·························································································· 2 IMPORTANT ······················································································· IMPORTANT-1 Warranty on Product ······································································ IMPORTANT-1 Installation, Relocation and After-sale Technical Service ····················· IMPORTANT-3 Technical Seminars and Training Courses for Users ·························· IMPORTANT-4 Estimated Life time of the Instrument ··············································· IMPORTANT-4 Caution on Disposal of Instrument ···················································...
Page 38 5. MAINTENANCE & INSPECTION ······································································ 5-1 Checking the Performance and Specifications·········································· 5-1 5.1.1 Preparation of Items ······································································ 5-1 5.1.2 Preparation of Operation ································································ 5-2 5.1.3 Reproducibility Check ···································································· 5-6 Periodic Maintenance ············································································ 5-8 5.2.1 Liquid Leak Check ········································································ 5-8 5.2.2 Washing the Flow Path ··································································...
Page 39 2. Place for Installation ······························································ APPENDIX1-3 Removal of Transport Fixed Plate (ChromasterUltra R 6270 Autosampler) ····························· APPENDIX1-4 Removal of Transport Screws (ChromasterUltra R 6430 DAD) ······································· APPENDIX1-5 Removal of Transport Screws (ChromasterUltra R 6440 FL Detector) ······························ APPENDIX1-6 Removal of Transport Screws (ChromasterUltra R 6420 UV-VIS Detector)························...
Page 40: Outline
1. OUTLINE The major features of the ChromasterUltra R 6270 Autosampler are as follows: Low carryover Low carryover is achieved by adoption of an effective washing process and reduction of the dead volume which causes residual of sample. The specification of carryover is ≦0.001%.
Page 41: Functions
2. FUNCTIONS 2.1 Name and Function of Each Part on Autosampler Main switch Window Door Figure 2-1 Front View (A) Main switch: Turns on/off power supply. Door: Front side open. Window: Used for visually confirm the instrument state. LED: There are four types of LEDs, and each LED lights in a specific color for the determined indication.
Page 42 2.1 Name and Function of Each Part on Autosampler Door status sensor Sample rack Syringe valve Syringe Injection valve Wash pump Rinse port 3-way solenoid valve Upper: Back flush side Lower: Wash pump side Figure 2-2 Front View (B) Sample rack: Accommodates sample vials.
Page 43 Needle Leak sensor Sample loop Injection port Figure 2-3 Front View (C) Needle: Aspirates a sample. Arm: Move right to left (X direction), back and forth (Y direction), and up and down (Z direction). Leak sensor: Detects fluid leakage. Injection port: A port into which the needle is inserted to inject a sample into the sample loop.
Page 44 2.1 Name and Function of Each Part on Autosampler External output terminal Wiring cover Grounding terminal Figure 2-4 Rear View Wiring cover: Removal of the left wiring cover gives access to the e-Line connector for connecting the units of ChromasterUltra Rs series. Grounding terminal: Grounds the autosampler.
Page 45: Operating Principle And Functional Description
2.2.1 2.2 Operating Principle and Functional Description 2.2.1 Configuration of Flow Path The configuration and the flow path of the ChromasterUltra Rs 6270 Autosampler is shown in Fig. 2-5 and 2-6. Sample vials are placed in the form of a rack. The sample rack moves along the transverse axis (X), and the arm moves along the longitudinal axis (Y) and the vertical axis (Z), whereby the needle can move freely among the sample vials, rinse port and injection port.
Page 46 2.2 Operating Principle and Functional Description Figure 2-6 Flow Path of Autosampler 2.2.2 2.2.3 2.2.4 2 - 6...
Page 47: Sample Injection Method
2.2.2 2.2.5 Sample Injection Method Description of each method Select a sample injection method for the ChromasterUltra Rs 6270 Autosampler from the following three methods. Table 2-1 Injection Methods Cut Injection All Volume Full Loop Method Injection Method Injection Method (Standard) Summary The amount of sample...
Page 48 2.2 Operating Principle and Functional Description NOTE: Depending on processing accuracy, the net capacity of a 1 µL loop is not uniform (± 30 %). Therefore, the area value of the Full Loop Injection Method differs from an equivalent amount of measured value of the Cut Injection Method and the All Volume Injection Method (up to twice).
Page 49 (For example, 1 µL loop is required 5 to 10 µL sample when measuring.) Wash fluid selection and use examples The ChromasterUltra Rs 6270 Autosampler uses two kinds of wash fluid. Refer to the following table. 2 - 9...
Page 50 2.2 Operating Principle and Functional Description Table 2-2 Isocratic Analysis One Fluid Wash Two Fluids Wash (for Cut injection method, (for All volume injection method, Low carry over analysis) Full loop injection method) Wash fluid 1 Sample solution Sample solution Mobile phase (Strong wash) Wash fluid 2...
Page 51: Sample Injection Procedure
2.2.3 2.2.3 Sample Injection Procedure Cut Injection Method Procedure The cut injection method, the injection volume, the lead volume, the rear volume, and the air volume are restricted by the following relational expression. A stroke of the syringe capacity can’t be used all. <70 µL of Standard syringe >...
Page 52 2.2 Operating Principle and Functional Description Idle status The mobile phase is flowing to the column from the pump via the loop of the injection valve. The wash fluid1 is connected to the syringe via the 3-way solenoid valve, the wash pump, and the port3 of the syringe valve.
Page 53 2.2.3 Aspiration of the wash fluid1 The syringe aspirates the dead volume (vd) (inside parameter) of fluid1. Switch the syringe valve to the rinse port side (port1) after aspiration of the wash fluid1. vd: Dead volume (the instrument inside parameter) vi: Injection volume (the sample net volume to the flow path) vl: Lead volume (forward part, cut volume) vr: Rear volume (rearward part, cut volume)
Page 54 2.2 Operating Principle and Functional Description When [wash before sample aspiration] is set. Deliver the wash fluid1 with the wash pump, move the needle into the rinse port, and rinse the needle outer wall (Figure 2- 11). Wash the injection port flow path with the wash fluid1 after the needle has moved into the injection port (Figure 2- 12).
Page 55 2.2.3 Figure 2-12 Washing the Injection Port Flow Path with the Wash Fluid1 2 - 15...
Page 56 2.2 Operating Principle and Functional Description (ii) When [wash before sample aspiration + fluid2 wash] is set. Deliver the wash fluid1 with the wash pump, move the needle into the rinse port, and rinse the needle outer wall (Figure 2- 11). Wash the injection port flow path with the wash fluid1 after the needle has moved into the injection port (Figure 2- 12).
Page 57 2.2.3 Sampling The syringe aspirates one-half of the air volume (va/2) while needle is moving to the sample vial. The syringe aspirates the rear volume (vr) + injection volume (vi) + lead volume (vl) of sample after the needle has moved into the sample vial. vd: Dead volume (the instrument inside parameter) vi: Injection volume (the sample net volume to the flow path) vl: Lead volume (forward part, cut volume)
Page 58 2.2 Operating Principle and Functional Description Wash of the needle outer wall with the wash fluid1 The syringe aspirates one-half of the air volume (va/2) while needle is moving to the rinse port. Deliver the wash fluid1 via the wash pump to rinse the needle outer wall.
Page 59 2.2.3 Movement of the needle, and discharge of the lead volume Move the needle into the injection port. The syringe discharges the lead volume (vl) + air volume (va/2) + dead volume (vd) of sample into the injection valve. vd: Dead volume (the instrument inside parameter) vi: Injection volume (the sample net volume to the flow path) vl: Lead volume (forward part, cut volume) vr: Rear volume (rearward part, cut volume)
Page 60 2.2 Operating Principle and Functional Description Pressure release (LOAD switching) Switch the injection valve to the LOAD side to release pressure in the loop. vd: Dead volume (the instrument inside parameter) vi: Injection volume (the sample net volume to the flow path) vl: Lead volume (forward part, cut volume) vr: Rear volume (rearward part, cut volume) va: Air volume (the air volume which sample is sandwiched.)
Page 61 2.2.3 Loading a sample The syringe discharges the injection volume (vi) of sample into the sample loop. vd: Dead volume (the instrument inside parameter) vi: Injection volume (the sample net volume to the flow path) vl: Lead volume (forward part, cut volume) vr: Rear volume (rearward part, cut volume) va: Air volume (the air volume which sample is sandwiched.) Figure 2-18 Discharging the Sample into the Sample Loop...
Page 62 2.2 Operating Principle and Functional Description Sample injection (INJECT switching) Switch the injection valve to the INJECT side from the LOAD side to load the sample into the flow path. Move the syringe to the top dead point (vr) after switching the injection valve.
Page 63 2.2.3 Wash of the injection port flow path with the wash fluid1 Switch the syringe valve to the needle side (Port2). Deliver the wash fluid1 via the needle to the injection port with the wash pump to wash the flow path of the buffer tube, the needle inner wall, and the injection valve.
Page 64 2.2 Operating Principle and Functional Description [When two wash fluids are used] Wash of the injection port flow path with the wash fluid2. Switch the 3-way solenoid valve to the wash fluid2 side. Deliver the wash fluid2 via the needle to the injection port with the wash pump to wash the flow path of the buffer tube, the needle inner wall, and the injection valve.
Page 65 2.2.3 Return to idle status Switch the syringe valve to the syringe side (Port3). Move the needle to the home position. Switch the 3-way solenoid valve of the wash pump side to the wash fluid1 side when two wash fluids are set. Figure 2-22 Returning to the Idle Status 2 - 25...
Page 66 2.2 Operating Principle and Functional Description [When the back flush wash for the injection port is used] Back flush wash with the wash fluid1. Switch the syringe valve to the back flush wash side (Port4). Switch the 3-way solenoid valve of the back flush wash side to the syringe valve side.
Page 67 2.2.3 All Volume Injection Method Procedure The all volume injection method, the injection volume, the feed volume, and the air volume are restricted by the following relational expression. A stroke of the syringe capacity can’t be used all. <70 µL of Standard syringe > vi + vf + va + 10 ≦...
Page 68 2.2 Operating Principle and Functional Description Idle status The mobile phase is flowing to the column from the pump via the loop of the injection valve. The wash fluid1 is connected to the syringe via the 3- way solenoid valve, the wash pump, and the port3 of the syringe valve.
Page 69 2.2.3 Aspiration of the wash fluid1 The syringe aspirates the feed volume (vf) + dead volume (vd) (inside parameter) of fluid1. Switch the syringe valve to the rinse port side (port1) after aspiration of the wash fluid1 (Figure 2-25). Figure 2-25 Switching the Syringe Valve to the Rinse Port Side (Port1) 2 - 29...
Page 70 2.2 Operating Principle and Functional Description When [wash before sample aspiration] is set. Deliver the wash fluid1 with the wash pump, move the needle into the rinse port, and wash the needle outer wall (Figure 2- 26). Wash the injection port flow path with the wash fluid1 after the needle has moved into the injection port (in the same procedure of Section 2.3.3 (i)).
Page 71 2.2.3 (ii) When [wash before sample aspiration + fluid2 wash] is set. Deliver the wash fluid1 with the wash pump, move the needle into the rinse port, and wash the needle outer wall (Figure 2- 26). Wash the injection port flow path with the wash fluid1 after the needle has moved into the injection port (Figure 2- 27).
Page 72 2.2 Operating Principle and Functional Description Figure 2-28 Washing the Injection Port Flow Path with the Wash Fluid2 2 - 32...
Page 73 2.2.3 Sampling The syringe aspirates one-half of the air volume (va/2) while needle is moving to the sample vial. The syringe aspirates the injection volume (vi) of sample after the needle has moved into the sample vial. Figure 2-29 Sampling 2 - 33...
Page 74 2.2 Operating Principle and Functional Description Wash of the needle outer wall with the wash fluid1 The syringe aspirates one-half of the air volume (va/2) while needle is moving to the rinse port. Deliver the wash fluid1 via the wash pump to wash the needle outer wall.
Page 75 2.2.3 Movement of the needle Move the needle into the injection port (Figure 2-31). Figure 2-31 Moving the Needle into the Injection Port 2 - 35...
Page 76 2.2 Operating Principle and Functional Description Pressure release (LOAD switching) Switch the injection valve to the LOAD side to release pressure in the loop. Figure 2-32 Releasing the Pressure (INJECT → LOAD Switching) 2 - 36...
Page 77 2.2.3 Loading a sample The syringe discharges the feed volume (vf) + injection volume (vi) + air volume (va) + dead volume (vd) of sample into the sample loop. vd: Dead volume (the instrument inside parameter) vi: Injection volume (the sample net volume to the flow path) vf: Feed volume (the wash fluid volume to deliver a sample to the loop) va: Air volume (the air volume which sample is sandwiched.) Figure 2-33 Discharging the Sample into the Sample Loop...
Page 78 2.2 Operating Principle and Functional Description Sample injection (INJECT switching) Switch the injection valve to the INJECT side to load the sample into the flow path. vd :Dead volume (the instrument inside parameter) vi :Injection volume (the sample net volume to the flow path) vf :Feed volume (the wash fluid volume to deliver a sample to the loop) va :Air volume (the air volume which sample is sandwiched.) Figure 2-34 Injecting the Sample (LOAD →...
Page 79 2.2.3 Full Loop Injection Method Procedure The full loop injection method, the injection volume, the waste volume, and the air volume are restricted by the following relational expression. A stroke of the syringe capacity can’t be used all. <70 µL of Standard syringe > vi + vw + va + 10 ≦...
Page 80 2.2 Operating Principle and Functional Description Idle status The mobile phase is flowing to the column from the pump via the loop of the injection valve. The wash fluid1 is connected to the syringe via the 3-way solenoid valve, the wash pump, and the port3 of the syringe valve.
Page 81 2.2.3 Aspiration of wash fluid1 The syringe aspirates the dead volume (vd) (inside parameter) of wash fluid1. Switch the syringe valve to the rinse port side (port1). When [wash before sample aspiration] is set, refer to the following operation. vd: Dead volume (the instrument inside parameter) vi: Injection volume (the sample net volume to the flow path) vw: Waste volume (the air volume of the lead and rear part of a sample) va: Air volume (the air volume which sample is sandwiched.)
Page 82 2.2 Operating Principle and Functional Description When [wash before sample aspiration] is set. Deliver the wash fluid1 with the wash pump, move the needle into the rinse port, and rinse the needle outer wall (Figure 2- 11). Wash the injection port flow path with the wash fluid1 after the needle has moved into the injection port.
Page 83 2.2.3 Sampling The syringe aspirates one-half of the air volume (va/2) while needle is moving to the sample vial. The syringe aspirates the waste volume (vw) + injection volume (vi) of sample after the needle has moved into the sample vial. vd: Dead volume (the instrument inside parameter) vi: Injection volume (the sample net volume to the flow path) vw: Waste volume (the air volume of the lead and rear part of a sample)
Page 84 2.2 Operating Principle and Functional Description Wash of the needle outer wall with the wash fluid1 The syringe aspirates one-half of the air volume (:va/2) while needle is moving to the rinse port. Deliver the wash fluid1 via the wash pump to wash the needle outer wall.
Page 85 2.2.3 Movement of the needle Move the needle into the injection port. Figure 2-39 Moving the Needle into the Injection Port 2 - 45...
Page 86 2.2 Operating Principle and Functional Description Pressure release (LOAD switching) Switch the injection valve to the LOAD side to release pressure in the loop. Figure 2-40 Releasing the Pressure (INJECT → LOAD Switching) 2 - 46...
Page 87 2.2.3 Loading a sample The syringe discharges the one-half of the air volume (va/2) + one-half of the waste volume (vw/2) + injection volume (vi) + dead volume (vd) (inside parameter) of sample into the sample loop. vd: Dead volume (the instrument inside parameter) vi: Injection volume (the sample net volume to the flow path) vw: Waste volume (the air volume of the lead and rear part of a sample) va: Air volume (the air volume which sample is sandwiched.)
Page 88 2.2 Operating Principle and Functional Description Sample injection (INJECT switching) Switch the injection valve to the INJECT side from the LOAD side to load the sample into the flow path. Move the syringe to the top dead point (vr) after switching the injection valve.
Page 89: Specifications
2.3.1 2.3 Specifications 2.3.1 Main Unit Maximum number of samples 120 (1.5 mL) 72 (4 mL) (option) 195 (1 mL) (option) 192 (96-holes microplate  2) (option) 768 (384-holes microplate  2) (option) Sample injection method Cut injection method All volume injection method Full loop injection method Syringe capacity 70 µL (standard)
Page 90 2.3 Specifications Carryover ≤ 0.001 % (Cut injection method) Linearity of injection volume : ≥0.999 (Cut injection method) (10) Injection volume accuracy ± 2 % (11) Minimum injection operating time Approx. 33 s (under specified condition) (12) Injection mechanism X, Y, Z directions: Needle movement (13) Washing method Washing with a dedicated pump Two Fluids wash function for needle inner wash...
Page 91 2.3.1 (18) GLP function Maintenance logbook Injection port seal Injection valve Syringe valve Syringe Wash pump operation time (19) External I/O START OUT (20) Dimensions 340 W  515 D  297 H (mm) (21) Weight Approx. 29 kg (22) Ambient temperature 4 to 35 C (non-condensing) (23) Ambient temperature variation 2 C/h or lower...
Page 92 2.3 Specifications (26) Power supply AC 100 to 240 V ± 10 %, 50/60 Hz, 310VA. *1) Performance specification should be checked in the following combination. Avoid using the non-slit septum or the sealed plastic cap to acquire original good reproducibility performance.
Page 93: Thermostat
2.3.2 2.3.2 Thermostat Temperature setup range* 1 to 45 °C (in increments of 1 °C) Temperature control range* [ambient temperature – 21 °C] to [ambient temperature + 25 °C] and within the setup range (humidity 60 %, sample vial 1.5 mL, 4 mL, 1 mL) [ambient temperature –...
Page 94 2.3 Specifications Temperature control system Peltier effect-based heating/cooling system Safety countermeasure Overheat prevention system by the thermal protector (75 °C) *1) An acceptable temperature range for the thermostat is from 1 to 45 °C. The actual temperature depends on ambient environment (temperature and humidity), so different specifications are set as temperature control ranges.
Page 95: System Operation
3. SYSTEM OPERATION Refer to instruction manual for ChromasterUltra Rs System "Section 3 SYSTEM OPERATION". 3 - 1...
Page 96: Operation
4. OPERATION The method of the ChromasterUltra Rs 6270 Autosampler operation is described here. Refer to each operation manual for the details of options, GUI controller, and CDS. WARNING Ignition of Flammable Chemicals This instrument is not explosion-proof. In unattended operation, do not use organic solvents having an ignition point below 70 C.
Page 97 4. OPERATION WARNING Inflammation or Injury due to Toxic, Corrosive or Stimulative Solvent When using a toxic, corrosive or stimulative solvent, be careful not to incur a physical inflammation or injury. For details of the properties of each solvent and how to handle it, refer to the relevant Safety Data Sheets (SDS).
Page 98: Basic Operation
4.1.1 4.1 Basic Operation 4.1.1 Power Supply The power activation operation of the ChromasterUltra Rs 6270 Autosampler is described here. Power switch Window Door Figure 4-1 Front Panel Initialize-processing: Press the power switch to turn on the power supply. And check of memory, arm drive mechanism, valve changeover mechanism, etc., and auto-adjustment of the starting point for...
Page 99: Measuring Operation
4.2 Measuring Operation 4.2 Measuring Operation WARNING Injury of Hand by Needle or Internal Mechanism Never touch the inside of the autosampler during operation, as your hand or finger may be seriously injured by the needle or internal mechanism. 4.2.1 Basics of Operation Handling sample rack For taking out the sample rack, slightly lift its gripping section, and then pull it out toward you.
Page 100 4.2.1 Make sure that the sample rack does not move in an attempt of pulling it toward the front side of the instrument. Rack holder Sample rack Gripping section Figure 4-2 Handling of Sample Rack NOTES: 1. Mount the sample rack at the correct position. If the rack is mounted at a wrong position, the instrument may be caused to malfunction.
Page 101 4.2 Measuring Operation Handling of Thermostat Rack When the thermostat for the ChromasterUltra Rs 6270 Autosampler is used at high temperature and humidity place, it may cause of dew condensation water in the sample rack. Remove the sample rack to prevent splash of dew condensation water to the instrument in the following procedure.
Page 102 4.2.1 Tilt the sample rack on the dried cloth and remove dew condensation water. Pull out the sample rack while keeping it in parallel position to the Rack holder Sample rack Handle Locating pin * Take care not to flaw these surfaces. Figure 4-3 Handling of Thermostat Rack 4 - 7...
Page 103 4.2 Measuring Operation Put it in until a samp ple vial touches the bottom of a sam mple rack. NOTES: 1. Exercise care not to drop off the e sample rack accidentally. 2. Take care not to flaw the conta cting surfaces of the sample rack and cooling rack h holder.
Page 104: Analyzing Operation
4.2.2 4.2.2 Analyzing Operation Routine analysis operation Make sure the pump, column, detector, data processing unit, etc. are correctly connected, and then set the mobile phase and detergent. Check that the waste bottle is not filled with waste solution. Start the pump and wait until the flow path is filled with the mobile phase.
Page 105: Preparatory Operations Of Autosampler Proper
4.2 Measuring Operation 4.2.3 Preparatory Operations of Autosampler Proper Check that the autosampler is correctly connected to the pump and detector before turning on the power. Check that the drain tube is correctly connected to the waste (solution) bottle. The waste bottle must be positioned at a level lower than the autosampler.
Page 106 4.2.3 (10) Click Wash button in “Needle Wash” of maintenance software to fill the needle and the injection port with wash fluid1. (When two wash fluids are used, replace the wash fluid2 side with fresh one). Then, make sure that the detergent flows normally, there are no leaks and the waste solution runs into the waste bottle.
Page 107: Maintenance & Inspection
5.1.1 5. MAINTENANCE & INSPECTION 5.1 Checking the Performance and Specifications Performance checking method is explained here. 5.1.1 Preparation of Items The following items are required for performance check. Component Units of Liquid Chromatography System  Pump (ChromasterUltra Rs 6170 Binary Pump) ...
Page 108: Preparation Of Operation
NOTE: Be sure to use the sample vials, caps and septa recommended by Hitachi High-Tech Science (refer to Section 7.3) If other parts are used, the instrument may be malfunction or its original performance may not be available.
Page 109 5.1.2 Mobile phase Wash fluid Column Oven Detector 40℃ Wavelength 273nm Time constant 0.1s SUS coil 0.25mmID×20m Autosampler (Injection valve) (Wash pump) （Degasser） Binary Pump (Waste solution bottle) Figure 5-1 Flow Path Diagram Connect the e-Line cable among the autosampler, detector and pump.
Page 110 5.1 Checking the Performance and Specifications Start the pump to fill each flow path in the pump and autosampler with liquid. After the mobile phase has reached the injection valve of autosampler, turn on the autosampler power supply. If the injection valve is switched over when no liquid is in it, the valve seal may be damaged.
Page 111 5.1.2 (12) Input the following contents as instrument conditions by using data processing software and maintenance software. For detailed operational information of data processing software and maintenance software, refer to the instruction manual attached to them. INJ.METHOD: LEAD VOLUME: REAR VOLUME: 20.0 ASP SYRINGE SPEED:...
Page 112: Reproducibility Check
5.1 Checking the Performance and Specifications 5.1.3 Reproducibility Check Check the reproducibility of the injection volume in the following procedure. Set the following conditions. The vial number on a rack: 1 1 μL Sample injection volume: Injection number of times: 6 times Prepare distilled water for the mobile phase and wash fluid.
Page 113 5.1.3 Data Check Determine the Relative Standard Deviation (RSD) of peak area by the following calculations.    Mean value     Standard deviation    Relative Standard Deviation xi: Area value due to each injection, n: Number of injections Make sure the RSD value is 0.50 % or less (injection volume is 1 µL).
Page 114: Periodic Maintenance
5.2 Periodic Maintenance 5.2 Periodic Maintenance Estimated periodic maintenance is based on full operation of 8 hours daily and 20 days monthly with standard assay. Check Item Frequency Procedure Liquid leak check Daily Refer to 5.2.1 Washing the flow path As required Refer to 5.2.2 Cleaning...
Page 115: Cleaning
5.2.3 When using a saline solvent When a saline solvent is used as a mobile phase, salt may precipitate in the flow path to clog it or scratch the seal face of valve. After using such a solvent, wash the flow path adequately with distilled water.
Page 116: Liquid Leak Sensor Check
5.2 Periodic Maintenance 5.2.5 Liquid Leak Sensor Check The ChromasterUltra Rs 6270 Autosampler is equipped with a liquid leak sensor. Refer to the following procedure and perform periodic operation check to detect liquid leakage correctly. Leak sensor operation check To check the operation of the leak sensor, drip a droplet of...
Page 117 5.2.5 Cleaning of the leak sensor Preparation: A hexagon wrench (P/N:N315003, Size:B2.5) Leak Sensor Leak Sensor Set Screw Drain Figure 5-3 Cleaning of the Leak Sensor Loosen the leak sensor set screw with the hexagon wrench. Detach the leak sensor from the drain tray. Wipe up droplets on the leak sensor and the drain tray.
Page 118: Drain Tray Check
5.2 Periodic Maintenance 5.2.6 Drain tray Check WARNING Inflammation or Injury due to Toxic, Corrosive or Stimulative Solvent When using a toxic, corrosive or stimulative solvent, be careful not to incur a physical inflammation or injury. For details of the properties of each solvent and how to handle it, refer to the relevant Safety Data Sheets (SDS).
Page 119: Wash Pump Check
5.2.7 5.2.7 Wash Pump Check Start the pump. After the mobile phase has reached each flow path in the pump and the injection valve of autosampler, turn on the autosampler power supply. If the injection valve is switched over when no liquid is in it, the valve seal may be damaged.
Page 120: Cleaning And Positioning The Rinse Port
5.2 Periodic Maintenance 5.2.8 Cleaning and Positioning the Rinse port WARNING Injury of Hand by Remover Tool When removing a plunger seal, plunger wash seal and plunger guide from the cylinder, the remover tool can injure your hand or finger. Carefully read this instruction manual before starting operation, and use care with remover tool.
Page 121 5.2.8 Move the needle to detach the rinse po ort. > Start maintenance software. Pres ss the [Connect] button. Check that the autosampler is co onnected. Press the [Maintenance] button to o select the autosampler tab. Press the [Function] button.
Page 122 5.2 Periodic Maintenance Detach the lid of wash tray. Detach the injection port with an injection port removal tool. Loosen the setscrew for Drain pipe to detach. Pull up the wash tray. And then loosen the setscrew for wash fluid pipe of rinse port to detach. Replace the wash tray with a new one, or clean it with an ultrasonic cleaner.
Page 123 5.2.8 (13) Using the HEIGHT command, lower the needle by about 5 mm. Select the autosampler tab in maintenance software. Press the [Function] button. Input [Z = 5.0] in the [Move X-Y] section. And then press the [Move] button.
Page 124: Consumables And Spare Parts
5.3 Consumables and Spare e Parts 5.3 Consumables and Spare Parts 5.3.1 Consumables The following parts are consumables. They m must always be on hand. The amount of consumption of each it tem is based on the assumption that the autosampler is operated d 20 days per month, 8 hours per day, and 3 minutes per analysis.
Page 125 5.3.1 Table 5-2 Consumables (continued) Location Standard Life Part No. Name Form / Specification (Estimated Life) 889-0532 Injection valve Injection valve 50,000 pod N switchings (One year) Serial Number of Autosampler(*): 1301T-001 to 1605T-010 889-0530 Injection valve pod Injection valve 40.000 switchings (One year)
Page 126: Spare Parts
5.3 Consumables and Spare e Parts 5.3.2 Spare Parts It is desired to prepare the items listed below w for running the system for a long time. Each item should be prepared in an adequate quantity depending on the operatio on time.
Page 127: Fastening Uhp Screws
5.3.3 5.3.3 Fastening UHP Screws UHP fitting (P/N: 889-0440) Double-end wrench (P/N: N311040, Size 1/4 x 5/16) Torque wrench (1/4, 2.5-3.5 Nm) (if any) Refer to the following figure, insert the UHP fitting into a connection part. Pipe Ferrule Backup ferrule Screw...
Page 128: Replacing Injection Port Seals
5.3 Consumables and Spare Parts 5.3.4 Replacing Injection Port Seals WARNING Injury of Hand by Remover Tool When removing a plunger seal, plunger wash seal and plunger guide from the cylinder, the remover tool can injure your hand or finger. Carefully read this instruction manual before starting operation, and use care with remover tool.
Page 129 5.3.4 Insert a seal remover into the injection port to remove an injection port seal and an o-ring. Insert a a seal remover into Seal remover the inje ection port. O-ring Injection port Injection port seal Remove an injection port seal. Figure 5-7 Removal of Injectio on Port Seal Attach a new injection port seal moiste...
Page 130 5.3 Consumables and Spare Parts NOTES: 1. Turn the taper side of the injection port seal to the injection port side when attach it. 2. Looseness of the injection port may cause malfunction of the instrument. Tighten the injection port firmly. After replacing the injection port seal, start maintenance software and click Maintenance - Autosampler tab - Wash for Needle Washing to carry out injection port cleaning (wash...
Page 131: Replacing Needles
5.3.5 5.3.5 Replacing Needles Items to be prepared: Wrench (5/16 inch, 12 mm) Flange needle (P/N 893-0816) Detach a sample rack from the rack tray. Start maintenance software. Click Maintenance. Click Autosampler tab. Click Needle Change. Using a wrench (5/16 inch) to detach the flange needle while holding the screw on the needle joint side with a wrench (12 mm).
Page 132 5.3 Consumables and Spare Parts Attach a new flange needle to the needle joint with your hand temporarily. Using a wrench (5/16 inch) to attach the flange needle while holding the screw on the needle joint side with wrench (12 mm). Check a setscrew.
Page 133: Replacing Syringes
5.3.6 5.3.6 Replacing Syringes Items to be prepared: Plastic syringe A tube between a plastic syringe and a syringe valve Syringe, Beaker, Distilled water, Wash fluid Syringe (70 µL) (P/N 893-0817) Syringe (175 µL) (P/N 893-0815) (option) Syringe (700 µL) (P/N 893-0845) (option) Detach the pipe connected to the port 4 of the syringe valve.
Page 134 5.3 Consumables and Spare Parts Connect the provided plastic syringe to the port4. Plastic syringe Figure 5-12 Connecting the Plastic Syringe (or Tube) Start maintenance software. Click Maintenance - Autosampler tab. Loosen the plunger setscrews with your hand to detach the syringe from the hook.
Page 135 5.3.6 Click IDLE (Air bubbles entered at the time of syringe mounting will be purged through the port 4 (BACK FLUSH). (10) Secure the plunger setscrews with your hand to fasten the plunger groove with the hook (11) Click Purge for Syringe Purge to replace fluid with wash fluid of the syringe.
Page 136: Discharging Air Bubbles From Syringes
5.3 Consumables and Spare Parts 5.3.7 Discharging Air Bubbles from Syringes Items to be prepared: Plastic syringe A tube between a plastic syringe and a syringe valve Detach the pipe from the port4 of the syringe valve. Pipe Syringe valve Figure 5-15 Syringe Valve Connect the provided plastic syringe to the port4.
Page 137 5.3.7 NOTES: 1. If it is difficult to eliminate air bubbles from the syringe, disconnect the syringe once (for details, refer to 5.3.6 Replacing Syringes). With 2-propanol, wash the inside of the syringe, and then try to eliminate air bubbles. 2.
Page 138: Replacing Injection Valve Pods
5.3 Consumables and Spare Parts 5.3.8 Replacing Injection Valve Pod Items to be prepared: Hexagonal wrench (1/4 inch) Hexagonal wrench (2mm, P/N N315001) Injection port removal tool (Remove tool) (P/N 894-1829) Injection valve pod (P/N 889-0530) Seal remover (P/N 889-0538) Injection port seal (P/N 889-0535) O-ring P7 (included in P/N 889-0535 injection port seal) Distilled water...
Page 139 5.3 Consumables and Spare Parts Pull up the wash tray. And then loosen the setscrews for wash fluid pipe of rinse port to detach. Loosen the hexagon socket head cap screw of the injection valve using a hexagon wrench (2mm) to detach the injection valve pod.
Page 140: Replacing Syringe Valve Seals
5.3 Consumables and Spare Parts 5.3.9 Replacing Syringe Valve Seals Items to be prepared: Hexagonal wrench (9/64 inch, This wrench is attached to P/N 893- 0814) Syringe valve seal (P/N 893-0814) Disconnect the tubes from the syringe valve with your hand. Port 1: Tube SV1 Port 2: Buffer tube Port 3: Tube SV3...
Page 141 5.3.9 Remove the syringe valve seal from the unit. Unit Syringe valve seal Syringe valve stator Stator ring Stator setscrew Figure 5-20 Removing the Syringe Valve Stator In the reverse order of removal, attach a new syringe valve seal, the stator ring, and the syringe valve stator. Then tighten the stator setscrews.
Page 142 5.3 Consumables and Spare Parts NOTE: Be sure to insert each tube into the inner side. If not, as tubes may be caught in the arm part(X, Y, Z mechanical part), it could result in the damage and malfunction of the arm part.
Page 143: Washing Solvent Filters
5.3.10 5.3.10 Washing Solvent F Filters The solvent filter (filter for wash fluid bottle) s should be washed in the following procedure. Items to be prepared: Beaker, Wash fluid (2-propanol) Ultrasonic cleaner, Distilled water Loosen the cap of the wash fluid bottle Inlet tube Wash fluid bot ttle...
Page 144 5.3 Consumables and Spare e Parts Fill a beaker with wash fluid (e.g. 2-pro opanol), and put the solvent filter in it. Perform ultrasonic cle eaning for approx. 5 to 6 minutes. Solvent filt Beaker Wash fluid Distilled water Ultrasonic cleaner Then, replace the wash fluid with distill ed water, and perform additional ultrasonic cleaning for appro...
Page 145: How To Replace Fuse
5.3.11 5.3.11 How to Replace Fu WARNING Electric Shock due to Contact with Ins side of Instrument When replacing the fuse with a new one, b be careful not to receive an electric shock. To prevent this, be sure to turn off the power switch before proceeding to fus e replacement.
Page 146: Troubleshooting
6.1.1 6. TROUBLESHOOTING 6.1 Troubleshooting 6.1.1 Troubleshooting Table If an abnormal phenomenon is encountered, reference should be made to the following table. Table 6-1 Troubleshooting Symptom Cause Remedy Error is a. Improper measuring a. Perform correct operation indicated on the conditions are entered or according to the message CDS/GUI.
Page 147 6.1 Troubleshooting Table 6-1 Troubleshooting (continued) Symptom Cause Remedy Reproducibility h. The detergent bottle is h. Wash the bottle and replace of analytical contaminated. the detergent. result is poor. i. The sample vial or septum is i. Replace the vial or septum. contaminated.
Page 148 6.1.1 Table 6-1 Troubleshooting (continued) Symptom Cause Remedy Carryover a. The flow path is a. Wash the flow path. occurs. contaminated. b. The injection port seal leaks. b. Check the lock nut and retighten The injection port is loose. if loose. Or replace the seal. c.
Page 149: Error Messages
Remedy ROM ERROR PC board malfunctions. Turn on power supply again. If the same error is indicated RAM ERROR again, contact your nearest LOG_INFORMATION service office of Hitachi High- ERROR Technologies Corporation sales EEPROM ERROR representative. PARAMETER ERROR COMMUNICATION Communication is abnormal.
Page 150 1. No sample vials are set. 1. Set sample vials. 2. The clearance between 2. Contact your nearest service the top face of sample vial office of Hitachi High- and the detection lever is Technologies Corporation inappropriate. Or the sales representative.
Page 151 3. Remove the obstacle. the moving route of syringe (vertically). 4. The electric system 4. Contact your nearest service malfunctions. office of Hitachi High- Technologies Corporation sales representative. ERROR-SVALVE 1. The syringe valve seal has 1. Replace the seal. worn out.
Page 152: Thermostat Errors
THERMO UNIT ROM PC board malfunctions. Turn on power supply again. ERROR If the same error is indicated again, contact your nearest THERMO UNIT RAM service office of Hitachi High- ERROR Technologies Corporation sales THERMO UNIT representative. PARAMETER ERROR THERMO UNIT TIME Communication is Turn on power supply again.
Page 153: Accessories
Rs 6270 Autosampler to meet the specific requirements of the laboratory. 7.1 Thermostat ChromasterUltra Rs 6270 Autosampler is equipped with thermostat as standard. This unit is capable of cooling 120 sample vials (1.5 mL each) within a temperature range from (room temperature - 21) °C to (room temperature + 25) °C, or a setting range.
Page 154: Sample Racks
7.2.1 Sample Racks Specification a) Sample rack for thermostat (4 mL x 72) i) Applicable product: ChromasterUltra RS 6270 Autosampler ii) Temperature setup range: 1 – 45 °C (in increments of 1 °C) iii) Temperature control range: [Ambient temperature -21 °C] to [Ambient temperature + 25 °C] and within the...
Page 155 Applicable sample vial: 4 mL (15 mm OD x 46 mm H) b) Sample rack for thermostat (1 mL x 195) i) Applicable product: ChromasterUltra RS 6270 Autosampler ii) Temperature setup range: 1 – 45 °C (in increments of 1 °C) iii) Temperature control range: [Ambient temperature -21 °C] to...
Page 156: Basic Operations Of 1M Rack
7.2 Sample Racks 7.2.2 Basic Operations of 1m Rack NOTICE Damage to Needle by Misssetting Sample Rack Cover If the rack cover is not pushed in completely, the instrument might malfunction. Be sure to use sample rack cover. Rack Cover Main body of the Rack Figure 7-2 Sample rack for 1 mL x 195 vials Setting of Microplate...
Page 157: Basic Operations Of Microplate Rack
7.2 Sample Racks 7.2.3 Basic Operations of Microplate Rack Setting of Microplate (a) Raise the rack cover. (b) Fit a microplate to the sample rack. (c) Push in the rack cover. (d) Mount the sample rack to the rack holder. Fit a microplate to the sample rack.
Page 158 7.2 Sample Racks Removal of Microplate (a) Remove the sample rack from the rack holder. (b) Raise the rack cover. (c) Take out the microplate from the sample rack. Use in Hot and Humid Environment If the microplate rack for the autosampler equipped with a thermostat is used in a hot and humid environment, the microplate may become difficult to remove because it may come in close contact with the aluminum block due to...
Page 159 Relationship between Set Temperature, Room Temperature and Offset Value NOTE: The offset values (Fig. 3-3) are set as typical values for the microplates recommended by Hitachi High- Technologies. When a stricter temperature control is needed, measure the liquid temperature in the wells...
Page 160 7.2 Sample Racks NOTE: If any microplate other than recommended by Hitachi High-Technologies is used, offset values may differ significantly from the above values. Furthermore, the thermostatic controllability intrinsic to the instrument will be unavailable. 7 - 8...
Page 161: Vials
NOTE: Be sure to use the sample vials, caps and septa recommended by Hitachi High-Tech Science (indicated in Table 7-4 to 12). If other parts are used, the instrument may malfunction or its original performance may not be available.
Page 162 Screw top Vials Table 7-4 Vials (A) 080-3704 855-2351 080-3710 080-3711 080-3595 080-3705 080-3596 890-3674 890-3675 1.5 mL 890-3676 pplicable Part No. Name Specification/Standard Remarks Rack 080-3595 1.5 mL sample e vial, Manufactured by Chromacol, Ltd. 92-0230 With blue 500 pcs contai Vial type: 2-SV, 92-0240 mark...
Page 163 7.3 Vials Table 7-5 Vials (B) 080-3704 855-2351 080-3710 080-3711 080-3706 1.1 mL pplicable Part No. Name Specification/Standard Remarks Rack 080-3706 1.1 mL sample e vial, Manufactured by Chromacol, Ltd. 92-0230 500 pcs contai Vial typel: 1.1-STVG, 92-0240 12  32 mm, Pointed-bottom type 855-2351 Septum (Si-TE...
Page 164 Table 7-6 Vials (C) 080-3702 080-1863 080-3707 080-3701 4 mL pplicable Part No. Name Specification/Standard Remarks Rack 080-3707 4 mL sample v ial, Manufactured by Chromacol, Ltd. 92-0232 Vial type: 4-SV,  500 pcs contai 92-0242 15  46 mm 080-3701 4 mL sample v Manufactured by Chromacol, Ltd.
Page 165 7.3 Vials Crimp top Vials Table 7-7 Vials (H) 080-3712 080-3597 080-3768 080-3598 1.5 mL pplicable Part No. Name Specification/Standard Remarks Rack 080-3597 1.5 mL crimp-to Manufactured by Chromacol, Ltd. 92-0230 With blue Vial type: 2-CV,  sample vial, mark 92-0240 12...
Page 166 Table 7-8 Vials (I) 080-3712 080-3700 1.1 mL pplicable Part No. Name Specification/Standard Remarks Rack 080-3700 1.1 mL crimp-to Manufactured by Chromacol, Ltd. 92-0230 sample vial, Vial type: 1.1-CTVG, 92-0240 12  32, 500 pcs contai Pointed-bottom type 080-3712 Crimp cap Manufactured by Chromacol, Ltd.
Page 167 7.3 Vials Crimp-top Vials A septum is placed in the aluminum crimp ca ap which is sealed to the vial using the dedicated crimper tool. To remove the cap, use the decapper. Crimper Part No. Name Remarks 080-3233 Crimper CR-11 For se ealing of 080-3712 Decapper...
Page 168 Neckless Vials Table 7-9 Vials (J) 890-3663 890-3662 890-3665 1 mL Applicable Part No. Name Specification/Standard Remarks Rack 890-3661 1 mL neckless Manufactured by 892-0234 With sample vial National Scientific Company, polyethylene 892-0244 (transparent), C4015-96 8 40 mm 200 pcs contained 890-3664 1 mL neckless Manufactured by...
Page 169 7.3 Vials Table 7-10 Vials (K) 080-1954 Applicable Part No. Name Specification/Standard Remarks Rack 080-1869 Neckless sample Manufactured by 892-0230 With vial (transparent), FUJIMOTO SCIENCE, silicone 892-0240 12  32 mm 100 pcs contained rubber cap 805-2236 Neckless sample Manufactured by Without vial (transparent), FUJIMOTO SCIENCE,...
Page 170 Snap Vials Table 7-11 Vials (L) 890-3670 890-3671 890-3669 890-3687 0.3 mL Applicable Part No. Name Specification/Standard Remarks Rack 890-3672 0.3 mL snap-top La-Pha-Pack® 892-0230 With snap sample vial, cap set, 11190933, 892-0240 100 pcs contained 11150637 having 12  32 mm, polypropylene septum (Si-PTFE, non-slit)
Page 171 Otherwise, the instrument performance may be degraded without the microplate seal. NOTE: Be sure to use the microplate recommended by Hitachi High-Tech Science. If other microplates are used, its original performance may not be available. 7 - 19...
Page 172: Optional Syringes, Sampling Loops
7.4 Optional Syringes, Sampling Loops Table 7-14 shows a list of the optional syringes, sampling loops for the ChromasterUltra Rs 6270 Autosampler. Refer to the Section 5.3.6 Replacing Syringes for detailed information. Table 7-14 Option Syringes and Sample Loops Syringe...
Page 173: Index
8. INDEX 3-way solenoid valve ..............2-2, 2-12, 2-24, 2-25, 2-26 Accessories ........................7-1 Advance-injection ......................2-51 All Volume Injection Method ..................... 2-27 Ambient humidity ......................2-51 Ambient temperature ......................2-51 Arm ............................. 2-3 Back flush wash ......................1-1, 2-26 Cap ...........................
Page 174 8. INDEX GLP ..........................2-51 Grounding terminal ......................2-4 Injection Method ..................2-7, 2-8, 2-9, 2-17 Injection port .................... 2-3, 5-18, 5-22, 5-32 Injection port removal tool ....................5-22 Injection port seal ................... 2-51, 5-18, 5-22 Injection valve ..................2-2, 2-51, 5-18, 5-32 Injection valve pod ....................
Page 175 Rack parameters ....................... 2-52, 5-5 Remove tool ......................5-22, 5-32 Reproducibility Check ......................5-6 Reproducibility of injection volume ..................2-49 Rinse port ..................2-2, 5-9, 5-14, 5-15, 5-33 Rinse Port Wash ....................... 4-11 Routine analysis operation....................4-9 RUN ........................... 2-1 Sample injection method ...................
Page 176 8. INDEX Vial ............................. 7-9 Wash fluid ........................... 2-2 Wash pump ......................2-2, 2-51, 5-8 Washing Pump Purge ....................... 4-10 Window..........................2-1 Wiring cover ........................2-4 8 - 4...
Page 177: Appendix
APPENDIX APPENDIX 1 INSTALLATION CAUTION Caution in Carrying Heavy Instrument ChromasterUltra R Organizer weighs as much as 9 kg. Be careful not to drop it and to handle it with care when carrying it, for preventing injury. Hold the right and left parts of the instrument firmly.
Page 178: Unpacking
If relocation of the instrument becomes necessary after delivery, consult your dealer or nearest Hitachi High- Tech Science service representative in order to avoid possible trouble involved in relocation.
Page 179: Place For Installation
2. Place for Installation For installation of the instrument, select a place which meets the following conditions. Operating temperature: Within 15 to 35 C and with minimum temperature variation (2 °C/h) during measurement. Utmost care should be used for preventing condensation. Operating humidity: 25 to 85% Atmospheric gas...
Page 180: Removal Of Transport Fixed Plate
(h) Power supply to motor-driven equipment (such as stirrer and vibrator) without a noise suppressor shall not be turned ON/OFF frequently on the same power supply line as that of this instrument. 2.1 Removal of Transport Fixed Plate (ChromasterUltra R 6270 Autosampler) Open the front door of ChromasterUltra R 6270...
Page 181: Removal Of Transport Screws
2.2 Removal of Transport Screws (ChromasterUltra R 6430 DAD) Remove Transport Screws from ChromasterUltra R 6430 Diode Array Detector. There are two screws for transport on the bottom of the detector. Remove them using a Phillips screwdriver and attach the washers with the accessory short screws at that place.
Page 182 2.3 Removal of Transport Screws (ChromasterUltra R 6440 FL Detector) Remove Transport Screws from ChromasterUltra R 6440 Fluorescence Detector. There are three screws for transport on the bottom of the detector. Remove them (screw for transport 1, 2, 3) using a Phillips screwdriver Screws for transport 3 (for fixing lamp house)
Page 183 Detach a lamp house from the detector (refer to section 5.6.1) Anode nut Lamp house New Xenon lamp Cathode nut Appended Fig. 1-4 Attaching Xenon Lamp Attach a Xenon lamp to the lamp house (refer to section 5.6.1). Adjust the position of xenon lamp (refer to section 5.6.1) NOTE: Please keep the transport screw after detaching.
Page 184 2.4 Removal of Transport Screws(ChromasterUltra R 6420 UV-VIS Detector) Remove grommets, please remove two screws for transport. Please attach two screws provided in screw holes. Please attach grommets to the original position. Grommets, Two Screws for tansport The screw on the optical base fixing Appended Fig.
Page 185: Items To Be Prepared By Customer
3. Items to be Prepared by Customer 3.1 Power Supply Arrangement Power supply voltage: 100 to 240 V AC Fluctuation shall be ±10% of the rated voltage. Frequency: 50 or 60 Hz Fluctuation shall be ±0.5 Hz of the rated frequency. Power Capacity: 1100 VA or more The total system shown comprises ChromasterUltra R...
Page 186 WARNING Electric shock due to improper grounding! This instrument is designed to meet the basic insulation stipulated in the international standard IE C61010-1. To prevent an electric shock hazard due to improper grounding, provide a proper grounding connection. Be sure to use the grounded 3P power cable, which is supplied as a standard accessory for the instrument.
Page 187 (a) Usual Grounding Connection (with grounded 3P power outlet) Grounded 3P power outlet (15 A) Grounded 3P table tap Power cord (attached to ChromasterUltra R instrument) 6170 Binary Pump 750 mm or Ground resistance 100  or less more below ground Connector Rear panel...
Page 188 (b) Connection when Grounded Power Outlet is Unavailable 2P power outlet (15 A) 3P table tap with grounding wire Power cord (attached to ChromasterUltra Rs Grounding instrument) 6170 Binary Pump terminal 750 mm or Ground more below resistance ground Connector 100 ...
Page 189: Installation Space
3.2 Installation Space For composing a liquid chromatograph system with the ChromasterUltra R System, a space of 735 mm wide and 765 mm deep is required at least. Behind each unit, a space of 150 mm or more must be secured. At the left and right sides of the respective units, a space of minimum 100 mm each is necessary.
Page 190: Assembling
5. Assembling NOTE: It is not permitted for the user to carry out installation at the time of delivery. For ensuring safety and high accuracy in operation of the instrument, installation of the instrument shall be carried out by or under supervision of qualified service personnel of the manufacturer or its authorized service agent.
Page 191: System Power Supply
5.1 System Power Supply For ChromasterUltra R System, the AC power cables are connected to each unit. Plug the power cables into AC power outlets on back side of instruments. ChromasterUltra R Organizer Inter Face Control Board ChromasterUltra R 6420 UV-VIS ChromasterUltra R 6430 DAD ChromasterUltra R...
Page 192: System Cabling
5.2 System Cabling In the ChromasterUltra R System, the module units are cabled by plugging the e-Line cable into the e-Line connector at the rear left of each unit. Rear panel side e-Line connector Appended Fig. 1-10 e-Line Cable Connector e-Line Cable Connection ChromasterUltra R Organizer...
Page 193 ChromasterUltra R Organizer Inter Face Control Board ChromasterUltra R 6420 UV-VIS ChromasterUltra R 6430 DAD ChromasterUltra R ChromasterUltra R 6310 Column Oven 6270 Autosampler ② ChromasterUltra R ChromasterUltra R 6170 Binary Pump 6440 FL Detector Appended Fig. 1-12 e-Line Cable Connection （with CM6440 FL Detector）...
Page 194 ChromasterUltra R Organizer Inter Face Control Board ChromasterUltra R 6420 UV-VIS ChromasterUltra R 6430 DAD ChromasterUltra R 6310 Column Oven ChromasterUltra R 6270 Autosampler ChromasterUltra R 6440 FL Detector ChromasterUltra R 6170 Binary Pump Appended Fig. 1-13 e-Line Cable Connection（with CM6440 FL Detector） (The FL detector is connected to a DAD (or UV-VIS detector) in parallel.) Appended Table 1-2 Details of Cable e-Line cable 1) (50 cm)
Page 195 USB Cable Connection ChromasterUltra R Organizer Inter Face Control Board ChromasterUltra R 6420 UV-VIS ChromasterUltra R 6430 DAD ChromasterUltra R ChromasterUltra R 6310 Column Oven 6270 Autosampler ChromasterUltra R ChromasterUltra R 6170 Binary Pump 6440 FL Detector To the USB connector of the PC Appended Fig.
Page 196 Appended Fig. 1-15 Driver Installation Screen, which is wrong APPENDIX 1 - 20...
Page 197: System Tubing
5.3 System Tubing In the ChromasterUltra R System, the inlet tube up to the pump is fastened to the left side face of each unit with tube clamps. ChromasterUltra R DAD System Flexible tube 400 Drain tube D Inlet tube A1 Inlet tube A2 Inlet tube B1 Inlet tube B2...
Page 198 Connection to tube 1. 6170 Binary Pump Connection to double corkscrew mixer Flexible tube 300 2. 6270 Autosampler Connection to injection valve (a) Flexible tube 300 (b) Flexible tube 400 3. 6310 Column Oven Connection to preheat plate (b) Flexible tube 400 (c) Flexible tube 180 or MEM column fitting 4.
Page 199 (2) ChromasterUltra R UV-VIS System Flexible tube 400 Drain tube Inlet tube A1 Inlet tube A2 Inlet tube B1 Inlet tube B2 Flexible tube 180 Inlet tube AS1 Inlet tube AS2 Inlet tube W Flexible tube 400 WP tube Drain tube P Flexible tube 300 Appended Fig.
Page 200 Drain Tubing (Right side view) (Front side view) T-joint T-joint Drain tube 1 Drain tube 1 T-joint T-joint Drain tube 2 Drain tube 2 Waste fluid tank P-OV tray Drain tube 3 Appended Fig. 1-18 Tubing Diagram for Drain Appended Table 1-6 Drain Tubes to be Used T-joint P/N 810-1362 (attached to autosampler)
Page 201 ChromasterUltra R Two Detectors System (DAD or UV-VIS detector + FL detector) The FL detector is connected to a DAD (or UV-VIS detector) in series. Flexible tube 400 Inlet tube A1 Inlet tube A2 Flexible tube 180 Inlet tube B1 Inlet tube B2 Inlet tube AS1 Inlet tube AS2...
Page 202 a) As for the analysis result of FL detector installed in the downstream side, a peak may become broad by the increase in system volume. b) Please be careful of the resisting pressure of the flow cell of DAD or UV-VIS detector installed in the upper stream side.
Page 203 ChromasterUltra R Two Detectors System (DAD or UV-VIS detector + FL detector) The FL detector is connected to a DAD (or UV-VIS detector) in parallel. Inlet tube A1 Flexible tube 400 Inlet tube A2 Inlet tube B1 Flexible tube 180 Inlet tube B2 INLET semi-micro U assy Inlet tube AS1...
Page 204: Fastening Between Component Units
5.4 Fastening between Component Units CAUTION Prevention of Instrument Tipover Personal injury could be incurred if the instrument tips over. When installing the instrument, provide a proper means for preventing the instrument from tipping over accidentally. On the rear of each module unit, a cabling cover is attached. The adjacent models are fastened with each other by attaching the fastening plate between the wiring covers.
Page 205: Communication
APPENDIX 2 DESCRIPTION ON CONTACT SIGNAL COMMUNICATION 1. Outline The following two types of contact signals are used in the ChromasterUltra R series. Contact signals (START, ERROR, BUSY) entering the e-Line connector. These are contact signals having input/output functions that are used in common for each unit. These signals are respectively formed from the START (IN/OUT), ERROR (IN/OUT), and BUSY (IN/OUT) lines.
Page 206 Connection with L-2000, Chromaster S Series Use 3-pin connectors of the e-DIO cab ble (890-6145) in the connection shown below. ERROR START STOP Line nector To L-2000, To ChromasterUltra R Chromaster series instrument series instrument e-Line connector BUSY 3-pin connec ctor e-DIO cable e-DIO cable...
Page 207: Individual Contacts Of Each Unit
3. Individual Contacts of Each Unit Contact Signal Input Terminals These are contact signal input termina ls for control using contact signals from an external switch, relay etc. Any of the signals is activated by shorting the term minal for 0.1 second or longer.
Page 208 +5 V 0  Max. 10 mA Chromaster series u unit External unit Activated u upon shorting contacts of external unit. Appended Fig. 2-3 Contact Signal Inpu ut Circuit 0.1 A max. 30 V max. Chromaster series u unit External unit Append ded Fig.
Page 209 APPENDIX 3 TERMINOLOGIES e-line Used for an analysis-specific digital network. Interface control board to control the ChromasterUltra R series instrument by PC. An interface control board (IFC board) is a standard equipment of the ChromasterUltra R Organizer. Liquid leak sensor A sensor to detect liquid leakage.
Page 210 Peltier A semiconductor device that allows heat exchange from one direction to another by flowing a current. Cooling or heating is possible according to the direction of current flow. Sample rack A component part on which a sample is set. Septum Sheet used as the lid for a vial.
Page 211 Auto zero Electrically adjusts absorbance to zero, thereby canceling a rise of the baseline. Generally activated just before or after start. Grating An optical device for dispersing incident beam according to differences in wavelength. Output range Determines the amplitude of a signal to be output to an external system such as data processor.
Page 212 Flow cell A sample container for measuring a sample continuously. Lamp house A section where the light source lamp is accommodated. The lamp gets hot when the instrument is used. Be careful not to get burned. Sample-side energy Refers to the intensity of beam that passes through a sample in a flow cell.

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