Thermo Scientific Dionex AS-DV Operator's Manual
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Thermo Scientific Dionex AS-DV Operator's Manual

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Dionex AS-DV Autosampler
Operator's Manual
Document No. 065259
Revision 05
October 2012
Table of Contents
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Summary of Contents for Thermo Scientific Dionex AS-DV

  • Page 1 Dionex AS-DV Autosampler Operator's Manual Document No. 065259 Revision 05 October 2012...
  • Page 2 Thermo Fisher Scientific Inc. All rights reserved. ©2012 Chromeleon is a registered trademark of Thermo Fisher Scientific Inc. in the United States. Adobe, Adobe Reader, and Acrobat are registered trademarks of Adobe Systems Incorporated in the United States and other countries. Microsoft, Windows, and Windows Vista are registered trademarks of Microsoft Corporation in the United States and other countries.

  • Page 3: Table Of Contents

    ....1 Overview of the Dionex AS-DV ....... 1 About This Manual .
  • Page 4 Creating an Instrument or a Program with the Wizard ..30 3.5.2 Dionex AS-DV General Parameters .....32 3.5.3 Basic Edit Mode .
  • Page 5 Contents 3.8.6 Inserting a Priority Sample ......48 3.8.7 Controlling the Delivery Volume ..... . . 49 3.8.8 Using Sample Overlap Mode .
  • Page 6 Facility Requirements ........87 Unpacking the Dionex AS-DV ....... .87 Connecting the Dionex AS-DV Sample Out Line .
  • Page 7 Verifying Chromeleon Communication ....102 B.8.5 Setting Up the Dionex AS-DV for Simultaneous Injections. . 102 C • Relay Control ......... . 103 TTL Input Function .
  • Page 8 Dionex AS-DV Operator’s Manual Doc. 065259-05 10/12...

  • Page 9: Introduction To The Dionex As-Dv

    Dionex ICS-5000, ICS-2100, ICS-1600, ICS-1100, and ICS-900 systems, as well as legacy IC systems. The Dionex AS-DV holds 50 vials (either 0.5 mL or 5.0 mL, or a combination of the two sizes). Vials can be sampled in any order and multiple samples can be taken from each vial.

  • Page 10: About This Manual

    ® ® Adob Acrobat or Adobe Reader for assistance. Chapter 1, Introduces the Dionex AS-DV; explains the conventions Introduction used in this manual, including safety-related information. Chapter 2, Describes Dionex AS-DVoperating features and the Description software required for autosampler control.

  • Page 11: Safety And Regulatory Information

    Thermo Scientific does not recommend the use of flammable solvents in the Dionex AS-DV vials. Operation of a Dionex AS-DV in a manner not specified by Thermo Scientific may result in personal injury.
  • Page 12 Dionex AS-DV Operator’s Manual Messages d’avertissement en français Signale une situation de danger immédiat qui, si elle n'est pas évitée, entraînera des blessures graves à mortelles. Signale une situation de danger potentiel qui, si elle n'est pas évitée, pourrait entraîner des blessures graves à mortelles.

  • Page 13: Safety Symbols

    1 • Introduction to the Dionex AS-DV 1.3.2 Safety Symbols The symbols below appear on the Dionex AS-DV or on Dionex AS-DV labels. Alternating current Primary protective conductor terminal Secondary protective conductor terminal Power supply is on Power supply is off Indicates a potential hazard.

  • Page 14: Declaration Of Conformity

    Dionex AS-DV Operator’s Manual 1.4.1 Declaration of Conformity The cETLus and CE marks on the Dionex AS-DV model/data label indicate that the autosampler is in compliance with the following standards. Doc. 065259-05 10/12...

  • Page 15: Description

    2 • Description Operating Features 2.1.1 Front View Figure 2-1 illustrates the main operating features of the Dionex AS-DV. Vial Carousel Carousel Cover Carousel Release Button (Not visible in photo) Sampling Head Tower LED Display Front Cover Figure 2-1. Dionex AS-DV Front View...
  • Page 16 Figure 2-1). • The sampling head tower delivers sample from the sample vials to the sample out line, which exits the front of the Dionex AS-DV (see Section 2.1.2). For a description of how the sampling mechanism operates, see Section 2.2.1.
  • Page 17 PolyVials or a combination of the two sizes. Vial adapters (P/N 071901, package of 50) are required to load 0.5 mL vials into the carousel. Adapters are included in the Dionex AS-DV Ship Kit for 0.5 mL vials (P/N AAA-071115). PolyVials serve both rinse and sample functions.
  • Page 18 Vial Tray Vial trays (either for 0.5 mL or 5.0 mL vials) are included in the respective Dionex AS-DV Ship Kit (P/N AAA-071115, Ship Kit for 0.5 mL vials; P/N 068887, Ship Kit for 5.0 mL vials). Place vials into the tray and then fill them until the liquid in the vials reaches the top of the tray.

  • Page 19: Front Interior Features

    Figure 2-4. Vial Tray (0.5 mL Version Shown) 2.1.2 Front Interior Features Figure 2-5 illustrates the front interior of the Dionex AS-DV. To access the front interior, remove the front cover by gripping the indentations at each side and pulling the cover straight off toward you. Sample...
  • Page 20 The sample out line is routed from the sampling mechanism through an aperture in the front interior. From there, it is connected either to the high- pressure valve in the Dionex AS-DV (if installed) or to the injection valve in the IC system.

  • Page 21: Rear Panel

    Power Receptacle Figure 2-6. Dionex AS-DV Rear Panel Drain Liquid from any spills exits the Dionex AS-DV enclosure through the port. Connect a drain hose (P/N 055075) to the port and place the DRAIN free end of the hose into either a waste container or an appropriate drain.
  • Page 22 Chromeleon is installed. Connect a USB cable between the USB connector on the Dionex AS-DV and a USB port on the PC. Alternatively, the Dionex AS-DV can be connected to a hub or an IC system with a USB port. For USB connection instructions, see Section B.6.

  • Page 23: Dionex As-Dv Operating Principles

    2 • Description Dionex AS-DV Operating Principles 2.2.1 Sampling Operating Principles The sampling head tower (see Figure 2-1) consists of a motor-driven needle, with a sampling tip on the end, that moves down to engage the top of the vial cap.

  • Page 24: Rinsing Operating Principles

    Section B.8.2). When performing a rinse, the Dionex AS-DV takes the rinse liquid from the first vial in the range. If the first vial is empty, the next vial in the range is used, and so on. To define rinse cycles in Chromeleon, see Section 3.8.3.
  • Page 25 2 • Description Figure 2-8 Figure 2-9 show the flow schematics for the 6-port and 10- port valves. B Position Figure 2-8. Optional 6-Port HIgh-Pressure Valve Flow Schematics B Position Figure 2-9. Optional 10-Port High-Pressure Valve Flow Schematics Operation as an Application Valve The optional valve can be configured for various application functions, including sample injection, concentrator column loading, or matrix elimination.
  • Page 26 Dionex AS-DV Operator’s Manual IC System AS-DV Injection Valve Optional Valve To Column (A Position) (Load Position) AS-DV Sample Out Line (5) S L (4) Sample Loop Concentrator (6) W C (3) Column (1) L P (2) PolyVial From Pump...

  • Page 27: Chromeleon Software

    2 • Description Chromeleon Software The Dionex AS-DV is controlled with a PC configured with Chromeleon 7 Chromatography Data System or Chromeleon 6.8 Chromatography Data System. 2.3.1 Software Control Modes Two modes of software control are available: direct control and automated control.

  • Page 28: System Wellness

    Dionex AS-DV Operator’s Manual Figure 2-13. Chromeleon 6.8 Panel Tabset: Dionex AS-DV Control Panel • With automated control, you create a predefined list of commands and parameters (called an instrument method in Chromeleon 7 or a program in Chromeleon 6.8) for controlling the Dionex AS-DV.
  • Page 29 2 • Description to monitor the usage of replaceable parts. After replacing a part, click Reset to reset the Current counter to 0. Figure 2-14. Dionex AS-DV Wellness Panel • The Carousel Jam Counter increments each time the carousel jams.
  • Page 30 Dionex AS-DV Operator’s Manual Doc. 065259-05 10/12...

  • Page 31: Operation And Maintenance

    3 • Operation and Maintenance This chapter describes routine operating and maintenance procedures for the Dionex AS-DV. Preparing the Sample Vials Thermo Fisher Scientific recommends thoroughly rinsing all PolyVials and caps with deionized water before filling to remove any traces of dust. Thermo Fisher Scientific DOES NOT recommend reusing vials or caps and does not guarantee their cleanness or proper operation if reused.

  • Page 32: Cleaning The Caps

    Dionex AS-DV Operator’s Manual 3.1.2 Cleaning the Caps Use the following procedure to clean caps without filters. For trace-level analyses, use caps without filters to avoid introducing contaminants from the filter. 1. Place caps into a clean 4-liter polyethylene container. Fill the container three-quarters full with deionized water and cap.

  • Page 33: Filling The Vials And Installing The Caps

    3 • Operation and Maintenance 3.1.4 Filling the Vials and Installing the Caps A cap must be installed in each vial in order for the autosampler to operate correctly. A vial without a cap will be sensed as an empty position.
  • Page 34 Cap Insertion Tool Figure 3-3. Caps Installed (0.5 mL Vials Shown) NOTE Do not use the concave end of the cap insertion tool for installing caps on Dionex AS-DV vials. It is for use with another autosampler. Doc. 065259-05 10/12...

  • Page 35: Turning On The Power

    Turning on the Power To turn on the autosampler power, press the power switch on the Dionex AS-DV rear panel (see Figure 2-6).

  • Page 36: Connecting To Chromeleon

    3. To start the Chromeleon 7 client, click Start > All Programs > Chromeleon 7 > Chromeleon 7. 4. To display the Dionex AS-DV ePanel, click the Instruments category bar on the Console. Click your instrument in the Navigation Doc. 065259-05 10/12...
  • Page 37 3 • Operation and Maintenance Pane. Chromeleon 7 connects to the instrument and displays the ePanel Set. Click the AS-DV tab (see Figure 3-4). Figure 3-4. Example Chromeleon 7 ePanel Set: Dionex AS-DV ePanel Doc. 065259-05 10/12...

  • Page 38: Connecting To Chromeleon 6.8

    Dionex AS-DV Operator’s Manual 3.4.2 Connecting to Chromeleon 6.8 1. Turn on the PC. 2. Check the Chromeleon 6.8 Server Monitor icon on the Windows taskbar and start the server, if it is not already running: • If the icon is gray , the server is already running (idle).
  • Page 39 3 • Operation and Maintenance 5. To display the Dionex AS-DV Control panel, click the AS-DV tab (see Figure 3-5). Figure 3-5. Example Chromeleon 6.8 Panel Tabset: Dionex AS-DV Control Panel Doc. 065259-05 10/12...

  • Page 40: Creating A Chromeleon Instrument Method Or Program

    A Chromeleon 7 instrument method or Chromeleon 6.8 program is a list of control commands to be executed at specified times, allowing automated operation of the Dionex AS-DV. This section provides an overview of how to create an instrument method or a program with a wizard. For detailed instructions, refer to the Chromeleon Help.
  • Page 41 AS-DV high-pressure valve configured as an injection valve, or the injection valve in the chromatography system module. The auxiliary valve is the optional Dionex AS-DV valve con- figured as an auxiliary valve. d. For Basic Edit Mode: Select the Volume of sample to deliver from the vial, select a Bleed option, and enable or disable a Rinse.

  • Page 42: Dionex As-Dv General Parameters

    The range is 0 to 500 µL. The default value of 125 µL is appropriate for the 1.4 m (56 in) length of sample out line provided with the Dionex AS-DV. You can set the Delay Volume in the Chromeleon 7 Instrument Configuration Manager or the Chromeleon 6.8...

  • Page 43: Basic Edit Mode

    Instrument Method Wizard or Program Wizard, a default list of commands is displayed in the Command/Parameters table. The Dionex AS-DV executes the commands in the order in which they appear in the table. Default Basic Edit Mode Commands •...

  • Page 44: Advanced Edit Mode

    Dionex AS-DV Operator’s Manual that will direct the bleed to waste. To disable the bleed, select None. For details about performing a bleed, see Section 3.8.2. • Rinse: Delivers rinse fluid through the sample out line. To enable the rinse, select the check box and then specify the volume. The volume is taken from the vials in the rinse vial range, specified in the Chromeleon 7 Instrument Method Manager or Chromeleon 6.8...
  • Page 45 3 • Operation and Maintenance The following two commands are available only if the optional valve is installed and configured as an auxiliary valve (see Section 2.2.3). • AuxValve.A: Sets the valve to the A position. • AuxValve.B: Sets the valve to the B position. Adding, Modifying, and Deleting Commands •...

  • Page 46: Creating A Chromeleon Sequence

    1. Start the Sequence Wizard: • For Chromeleon 7: On the Console, on the menu bar, click Create. Select the instrument in which the Dionex AS-DV is configured and click Next >. • For Chromeleon 6.8: Select File > New. Select Sequence (using Wizard) and click OK.
  • Page 47 3 • Operation and Maintenance 3. On the last page of the wizard, click Finish (Chromeleon 7) or Done (Chromeleon 6.8). The sequence opens on the Chromeleon 7 Console (see Figure 3-6) or in the Chromeleon 6.8 Browser (see Figure 3-7).

  • Page 48: Starting A Run

    During a run, do not press the Carousel Release button. Doing so clears all current vial information from Dionex AS-DV memory. (During a run, the Dionex AS-DV stores the vial size installed in each carousel position and the remaining volume in each vial.) After creating the sequence, you can start a run.

  • Page 49: Specifying A Bleed

    3 • Operation and Maintenance • To interrupt the autosampler when a sequence is not running, click Stop on the Dionex AS-DV ePanel in Chromeleon 7 or the Dionex AS-DV Control panel in Chromeleon 6.8. NOTE After the Dionex AS-DV stops its current operation, the carousel returns to the home position (position 1).
  • Page 50 Dionex AS-DV Operator’s Manual Figure 3-9). For suggested valve plumbing connections, see “Plumbing the Optional Valve” on page Figure 3-8. Sample Preparation Options (Chromeleon 6.8 Version Shown): Bleed Enabled Using the Injection Valve Doc. 065259-05 10/12...
  • Page 51 3 • Operation and Maintenance Figure 3-9. Sample Preparation Options (Chromeleon 6.8 Version Shown): Bleed Enabled Using the Auxiliary Valve Selecting Bleed adds two commands to the Command/Parameters table (see Figure 3-8 Figure 3-9): • The valve command switches the valve position to direct the bleed volume to waste.

  • Page 52: Specifying A Rinse

    Dionex AS-DV Operator’s Manual 3.8.3 Specifying a Rinse A rinse delivers rinse liquid from a vial to waste. To perform a rinse cycle, the Dionex AS-DV needs the following information: • The position of the vial containing the rinse liquid •...
  • Page 53 3 • Operation and Maintenance Server Configuration program (see “Specifying the Rinse Vial Position” on page 45). Figure 3-10. Sample Preparation Options (Chromeleon 6.8 Version Shown): Deliver Rinse in Basic Edit Mode Doc. 065259-05 10/12...
  • Page 54 Dionex AS-DV Operator’s Manual Selecting the Rinse check box and entering a Volume adds two commands to the beginning of the Command/ Parameters table (see Figure 3-10): • The InjectPosition command switches the valve position, directing the rinse volume to waste.
  • Page 55 Figure 3-10. When the Dionex AS-DV performs the rinse, it takes the rinse liquid from the first vial in the rinse vial range. If the first vial is empty, the next vial in the range is used, and so on. If the specified rinse volume is greater than a single vial volume, the Dionex AS-DV takes the Doc.
  • Page 56 Dionex AS-DV Operator’s Manual volume from more than one vial. Verify that the specified volume is available in the vials in the rinse vial range. Method 2: Enter a specific vial position in the Chromeleon instrument method or program. 1. On the Sample Preparation page of the Instrument Method Wizard/Editor or Program Wizard/Editor, select Advanced Edit Mode.

  • Page 57: Returning To A Vial

    3-14, Sample 1 is taken from the vial in position 1. Samples 2 and 3 are taken from the vials in positions 2 and 3, respectively. For Sample 4, the Dionex AS-DV returns to the vial in position 1. Figure 3-14. Chromeleon Sequence Example (Chromeleon 6.8 Version Shown): Returning to a Vial...

  • Page 58: Inserting A Priority Sample

    A message informs you that a queue or batch is currently running. Click Yes to continue. 4. Wait until the Dionex AS-DV carousel and sampling tip are not moving. Then lift the top cover, remove the old vials, and replace them with the new vials.

  • Page 59: Controlling The Delivery Volume

    3 • Operation and Maintenance 3.8.7 Controlling the Delivery Volume The Dionex AS-DV can deliver a full vial volume in one injection or deliver a partial vial volume in multiple injections. Sample delivery volume features vary, depending on the Edit Mode.
  • Page 60 • Full: The Dionex AS-DV delivers the full vial volume. • 1666 L: The Dionex AS-DV delivers 1666 L from the vial. Select this option if you want to take three injections from a 5000 L vial. • 2500 L: The Dionex AS-DV delivers 2500 L from the vial.
  • Page 61 3 • Operation and Maintenance For example, to take five injections from a filled 5.0 mL vial, select 1000 L. In the instrument method Script or program Commands view, a DeliverSample Volume=1000 command appears (see Figure 3-16). Figure 3-16. Chromeleon 6.8 Commands View Example: Deliver Multiple Injections Per Vial by Specifying the Volume Notes: •...
  • Page 62 Example: Using the From Sequence Volume Option When From Sequence is selected for the sample delivery volume, the Dionex AS-DV calculates the delivery volume using the formula shown in the Total Volume Delivered Estimator. In this case, the DeliverSample command is included without a specified volume.

  • Page 63: Using Sample Overlap Mode

    Dionex AS-DV always injects a full loop volume. 3.8.8 Using Sample Overlap Mode The Dionex AS-DV can be configured to overlap sample preparation functions. This means that while data collection is occurring for one injection, the Dionex AS-DV performs the sample preparation steps for the next injection in the sequence.
  • Page 64 Dionex AS-DV Operator’s Manual Program Wizard, Chromeleon automatically adds the EndSamplePrep and BeginOverlap commands. See the example in Figure 3-18. Figure 3-18. Chromeleon 6.8 Commands View Example: Sample Overlap Enabled Doc. 065259-05 10/12...
  • Page 65 3 • Operation and Maintenance Enabling Sample Overlap Mode In the Instrument Configuration Manager or Server Configuration program, open the Dionex AS-DV Properties dialog box and click the Options tab. Select the Sample Overlap check box (see Figure 3-19). Figure 3-19. Dionex AS-DV Configuration Properties: Options Tab Page with Sample Overlap Enabled Doc.

  • Page 66: Routine Maintenance

    Routine Maintenance This section describes routine maintenance procedures that the user can perform for the Dionex AS-DV. Any maintenance procedures for the Dionex AS-DV that are not described here must be performed by Thermo Fisher Scientific personnel. For assistance, contact Technical Support for Dionex products. In the U.S. and Canada, call 1-800-346-6390.

  • Page 67: Troubleshooting

    4 • Troubleshooting This chapter is a guide to troubleshooting problems that may occur while operating the Dionex AS-DV. • Section 4.1 describes the error messages displayed in the Chromeleon audit trail. • Section 4.2 through Section 4.8 describe other operating problems and how to resolve them.
  • Page 68 Dionex AS-DV Operator’s Manual The table below lists the Dionex AS-DV error messages. For troubleshooting assistance, refer to the page indicated in the table Alarms and Error Conditions Default Severity Level Attempt to deliver an invalid vial. Error page 59 Attempt to reset vials while needle is not home.
  • Page 69 This error occurs if you attempt to reset vial memory, either by pressing the button or clicking a Reset Vial Memory button on the Carousel Release Dionex AS-DV Control panel (see Figure 3-5), when the needle is down. To troubleshoot: 1.
  • Page 70 This error occurs when the needle is jammed or there is excessive backpressure. The motor speed is reduced and the Dionex AS-DV attempts to continue delivery. If the problem persists, the run is stopped, and the “Needle is jammed”...
  • Page 71 4 • Troubleshooting Needle is jammed. This error occurs when the needle is jammed or there is excessive backpressure. To troubleshoot: 1. Press the button to release the carousel and raise the Carousel Release needle. If the needle does not raise automatically, manually raise the needle (see Section 5.4).
  • Page 72 TTL triggered while another command is in progress. This error occurs if a controlling device sends a signal to the TTL input while the Dionex AS-DV is already performing a command. To troubleshoot: 1. After the other command is completed, send the signal to the TTL input again.
  • Page 73 NOTE Turning off the Dionex AS-DV power does not reset vial memory. c. On the Dionex AS-DV ePanel or Control panel, try to toggle the valve position by clicking first the Load Position (or Position A) button and then the Inject Position (or Position B) button.

  • Page 74: Liquid Leaks

    Dionex AS-DV memory. (For example, a 5.0 mL vial is installed in position 10, but the Dionex AS-DV expects a 0.5 mL vial to be installed in that position.) Vial memory must be reset before starting a new run.

  • Page 75: Poor Peak Reproducibility

    4 • Troubleshooting • Loose or damaged fitting connection(s) to optional high-pressure valve in the Dionex AS-DV or injection valve in the IC system 1. Make sure the liquid lines to the valve are tight. Replace any damaged fittings. See Section 5.1...

  • Page 76: Unexpected Or Extraneous Peaks Detected

    Dionex AS-DV Operator’s Manual • Liquid leak 1. Do not reuse caps or sample vials. 2. Inspect the lip and socket of each new cap for irregularities, nicks, scratches, or other damage that might interfere with sealing as the cap is pushed into the vial.

  • Page 77: Autosampler Stops Operating

    Excessive Backpressure • Injection valve in wrong position On the Dionex AS-DV ePanel (in Chromeleon 7) or Control panel on the panel tabset (in Chromeleon 6.8), toggle the valve position. • Restriction in the liquid flow path 1. If the high backpressure persists, follow the liquid flow path backward from the injection valve, through the high-pressure valve (if installed), loosening fittings and reconnecting them until you locate the restriction.

  • Page 78: Autosampler Jammed

    Sampling tip jams as it is being withdrawn 1. Check for excessive backpressure (see Section 4.7). 2. Check the sampling tip for possible obstruction: a. Turn off the power to the Dionex AS-DV. b. Manually raise the sampling head (see Section 5.4). c. Check for blockages.

  • Page 79: Service

    Refer to the warranty statement in the Dionex Terms and Conditions for more information. Replacing Tubing and Fittings The Dionex AS-DV is plumbed with the needle and tubing assembly listed below: Tubing Assembly Used for Dionex AS-DV Sampling Tip Kit...

  • Page 80: Removing The Carousel

    2. Turn off the Dionex AS-DVpower and disconnect the power cord. Raise the top cover. 3. Remove the Dionex AS-DV front cover by gripping the indentations at each side and pulling the cover straight off toward you. 4. Use a #1 Phillips screwdriver to remove the three flathead screws from the...
  • Page 81 5 • Service 5. Use a 3-mm Allen wrench to remove the set screw on the sampling head (see Sampling Figure 5-2). Head Remove the set screw Figure 5-2. Removing the Set Screw 6. Pull slightly on the sample out line to gain a small amount of slack in the line.
  • Page 82 Dionex AS-DV Operator’s Manual 8. Lift the needle out of the carousel and set it to the right (see Figure 5-4). Lift the needle Set the needle to the right Figure 5-4. Lifting the Needle Out of the Carousel 9. Use a 2.5-mm Allen...
  • Page 83 5 • Service 10. Unscrew the thumbscrew on the carousel, and lift the carousel out of the autosampler. Figure 5-6. Removing the Carousel 11. Clear any blockages and clean the basin. 12. Align the holes on the bottom of the carousel Alignment Pins on Carousel with the pins on the top of the carousel shaft and reinstall the carousel.

  • Page 84: Replacing The Sampling Tip And Tubing

    Carousel Release position. 3. Turn off the Dionex AS-DV power and disconnect the power cord. Raise the top cover. 4. Remove the Dionex AS-DV front cover by gripping the indentations at each side and pulling the cover straight off toward you.
  • Page 85 5 • Service 6. Use a 3-mm Allen wrench to remove the set screw on the sampling head (see Sampling Figure 5-8). Head Remove the set screw Figure 5-8. Removing the Set Screw 7. Pull slightly on the sample out line to gain a small amount of slack in the line.
  • Page 86 Dionex AS-DV Operator’s Manual 11. Thread the free end of the tubing up through the needle and screw the tip into place on the needle. 12. Continue threading the free end of the tubing up through the sampling head and out the aperture on the front of the Dionex AS-DV.

  • Page 87: Manually Raising The Sampling Head

    1. Turn off the Dionex AS-DVpower and disconnect the power cord. Raise the top cover. 2. Remove the Dionex AS-DV front cover by gripping the indentations at each side and pulling the cover straight off toward you. 3. Use a #1 Phillips screwdriver to remove the three flathead screws from the...
  • Page 88 Dionex AS-DV Operator’s Manual 4. Insert a 2.5-mm Allen wrench into the screw on top of the sampling mechanism (see Figure 5-11) and turn the wrench clockwise. The needle begins moving up. 5. Continue turning the wrench until the top of the sampling head is even with the top of the sampling mechanism.

  • Page 89: Rebuilding A High-Pressure Valve

    The following tool is needed to complete this procedure: 3-mm Allen wrench. 1. Turn off the Dionex AS-DVpower. The Dionex AS-DV power must be turned off before you rebuild a valve. If the power is on when you disconnect or connect the valve cable, the Dionex AS-DV will be damaged.
  • Page 90 Dionex AS-DV Operator’s Manual 5. Remove the 6-port or 10-port high-pressure valve: a. Use a 3-mm Allen wrench to remove the two screws holding the valve assembly in place (see Figure 5-13). Remove these 2 screws Figure 5-13. Removing the Valve Assembly b.

  • Page 91: Replacing A High-Pressure Valve Pod

    Dionex AS-DV may be damaged. 2. Raise the top cover. 3. Remove the Dionex AS-DV front cover by gripping the indentations at each side and pulling the cover straight off toward you. 4. Disconnect each liquid line connected to the valve.
  • Page 92 Dionex AS-DV Operator’s Manual 5. Remove the 6-port or 10-port high-pressure valve: a. Use a 3-mm Allen wrench to remove the two screws holding the valve assembly in place (see Figure 5-15). Remove these 2 screws Black Locking Ring Figure 5-15. Removing the Valve Assembly b.
  • Page 93 11. Reinstall the valve assembly and reconnect all liquid lines. 12. Turn on the power to the Dionex AS-DV. 13. Start a test run to check for leaks from the valve. Tighten fittings as required. 14. Reinstall the front cover and close the top cover.

  • Page 94: Replacing The Main Power Fuses

    Dionex AS-DV Operator’s Manual Replacing the Main Power Fuses 1. Turn off the power switch on the Dionex AS-DV rear panel (see Figure 5-18). 2. Disconnect the main power cord from both its source and from the Dionex AS-DV rear panel.

  • Page 95: A • Specifications

    A • Specifications A.1 Electrical Main Power 100 to 240 Vac, 50 to 60 Hz, 45 W Typical input power: 15 W Maximum line draw: 0.22 A at 110 Vac Fuses Two fast-blow IEC 127 fuses rated at 3.15 A (P/N 954745) A.2 Physical Dimensions Width: 44.45 cm (17.5 in)

  • Page 96: Sampling Mechanism

    Dionex AS-DV Operator’s Manual A.4 Sampling Mechanism Carousel 50 vials Sample Capacity Materials All wetted surfaces are chemically inert plastic (polypropylene, ™ ® polyethylene, polytetrafluoroethylene, PEEK , Tefzel , or Kel- ® Delivery Type Programmable in Chromeleon: From 0.1 mL/min to 5.0 mL/min...

  • Page 97: B • Installation

    For optimum operation, place the Dionex AS-DV close as possible to the left side of the IC system. • Allow at least 38 cm (15 in) of free space behind the Dionex AS-DV for connections and ventilation. •...

  • Page 98: Connecting The Dionex As-Dv Sample Out Line

    1. Raise the Dionex AS-DV top cover. 2. Remove the Dionex AS-DV front cover by gripping the indentations at each side and pulling the cover straight off toward you. The sample out line is threaded through an aperture in the chassis (see Figure B-1).

  • Page 99: Connecting The Sample Out Line Directly To An Ic System

    Section B.3.2). B.3.1 Connecting the Sample Out Line Directly to an IC System 1. Route the sample out line through the side slot in the Dionex AS-DV chassis. Leave a little slack in the tubing. Route sample out line through...
  • Page 100 Installing the Optional Valve 1. Verify that the power to the Dionex AS-DV is off. The Dionex AS-DV power must be turned off before you install a valve. If the power is on when you connect or disconnect the valve cable, the Dionex AS-DV will be damaged.
  • Page 101 4. Remove the 6-port or 10-port valve from the Dionex AS-DV Valve Kit (6-port, P/N 068920; 10-port, P/N 068921). 5. Align the electrical cable with the connector on the back of...
  • Page 102 • Plumbing the 10-port valve as a bleed valve (see Figure B-7). For information about plumbing the valve for other functions, refer to the appropriate Thermo Scientific Dionex Application Note. To Column AS-DV Sample Out From Pump Sample Loop To Waste Figure B-5.
  • Page 103 Dionex AS-DV and is not elevated at any point. 2. Connect the Dionex AS-DV sample out line: a. Install a fitting and ferrule onto the end of the Dionex AS-DV sample out line. b. Connect the sample out line to the appropriate port on the valve:...

  • Page 104: Connecting The Drain Line

    5. Replace the front cover and close the top cover on the Dionex AS- Go on to Section B.4. B.4 Connecting the Drain Line 1. Locate the corrugated drain hose (P/N 055075) in the Dionex AS-DV Ship Kit. 2. Push the hose onto the port on the DRAIN Dionex AS-DV rear panel.

  • Page 105: Installing Chromeleon And The Chromeleon Software License

    USB ports. • Use a hub if the Dionex AS-DV is more than 5 meters (16 ft) from the PC. The USB standard limits the USB cable length to 5 meters (16 ft). Each USB device can be separated from the PC by no more than five hubs.

  • Page 106: Connecting The Power Cord

    Dionex AS-DV Operator’s Manual B.7 Connecting the Power Cord 1. Verify that the power switch on the rear panel of the Dionex AS-DV is turned off. (The power switch may be turned on accidentally when the module is unpacked.) 2. Connect the power cord (IEC 320 C13) (ordered separately) from the main power receptacle on the rear panel to a grounded power source.

  • Page 107: Setting Up Chromeleon

    Chromeleon 6.8 Server is starting. 5. Press the power switch on the Dionex AS-DV rear panel to turn on the power. 6. Windows automatically detects the new USB devices. A message flashes on the screen to inform you that new hardware was found.

  • Page 108: Configuring The Dionex As-Dv In Chromeleon

    • For Chromeleon 6.8, click Start on the Windows taskbar and click All Programs > Chromeleon > Server Configuration. 2. Add the Dionex AS-DV to an existing instrument or timebase or create a new instrument or timebase. Doc. 065259-05 10/12...
  • Page 109 B • Installation 3. In the Dionex AS-DV Properties dialog box, click the Options tab (see Figure B-8). Figure B-8. Dionex AS-DV Properties Dialog Box: Options Tab Page 4. If a high-pressure valve is installed, select the function for which the valve will be used: •...

  • Page 110: Connecting To The Epanel Set Or Panel Tabset

    Console. Click your instrument in the Navigation Pane. Chromeleon 7 connects to the instrument and displays the ePanel Set. Click the AS-DV tab (see Figure B-9). Figure B-9. Example Chromeleon 7 ePanel Set: Dionex AS-DV ePanel Doc. 065259-05 10/12...
  • Page 111 View > Default Panel Tabset or click the Default Panel Tabset toolbar button. 3. To display the Dionex AS- DV Control panel, click the AS-DV tab (see Figure B-10). Figure B-10. Example Chromeleon 6.8 Panel Tabset: Dionex AS-DV Control Panel Doc. 065259-05 10/12...

  • Page 112: Verifying Chromeleon Communication

    Verifying Chromeleon Communication To verify that Chromeleon is communicating with the Dionex AS-DV: 1. On the Dionex AS-DV ePanel (in Chromeleon 7) or Control panel (in Chromeleon 6.8), under Settings, enter a Vial Position. 2. Under Commands, click Move to Vial. The carousel should move to the entered position.

  • Page 113: C • Relay Control

    C-1) on the rear panel of the Dionex AS-DV provides one relay output and one TTL input. Figure C-1. Relay Output and TTL Input Connector (on Dionex AS-DV Rear Panel) The relay output can be programmed in Chromeleon to switch any low-voltage device.

  • Page 114: Ttl Input Function

    When the input is triggered for the first time, the Dionex AS-DV performs a full vial delivery on vial position 1. When triggered again, the Dionex AS-DV performs a full vial delivery on vial position 2, and so on.

  • Page 115: Ttl Input Control Modes

    If the Dionex AS-DV has been connected to Chromeleon, the delivery speed, flush factor, and TTL homing delay last entered are used. To change one of the options, you can connect the Dionex AS-DV to Chromeleon, change the option’s value, and then disconnect the Dionex AS-DV from Chromeleon.
  • Page 116 • For Chromeleon 6.8, click Start on the Windows taskbar and click All Programs > Chromeleon > Server Configuration. 2. Under the instrument or timebase, double-click the Dionex AS-DV icon. The Dionex AS-DV Properties dialog box appears. 3. Click the TTL Inputs tab and then press the F2 key.
  • Page 117 C • Relay Control The Device Configuration dialog box appears (see Figure C-2). Figure C-2. Device Configuration Dialog Box: Selecting the TTL Input Control Mode 4. Select the TTL input control mode required by the controlling device from the Mode list. Doc.

  • Page 118: Connecting To The Relay Output And Ttl Input

    When attaching wires to the connector plug, be careful not to allow stray strands of wire to short to an adjoining position on the connector. 3. Plug the connector into the 4-pin connector on the Dionex AS-DV rear panel (see Figure C-1).

  • Page 119: Example Connections For Stand-Alone Operation

    TTL-2 TTL-3 TTL-4 Figure C-4. Example TTL Input Connection: Dionex AS-DV Connected to Dionex IC20 To control the Dionex AS-DV, set up a Dionex IC20 method that includes timed events for triggering on the autosampler. Figure C-5 illustrates the timed TTL IN events in an example method.

  • Page 120: Controlling The Relay Output

    Figure C-5. Dionex IC20 Timed Events Example C.4 Controlling the Relay Output The Dionex AS-DV provides relay contacts for control of functions in external devices. The relay output can be programmed in Chromeleon to switch any low- voltage device. Switched current must be no more than 200 mA at 5 VDC. The relay-contact closures are normally open.
  • Page 121 C • Relay Control Controlling the Relay Output Directly Using Chromeleon 7 Open the ePanel Set (see Section 3.4.1) and press the F8 key. The Command window appears. 2. In the list of devices in the left pane, click Sampler_Relay. 3.
  • Page 122 Dionex AS-DV Operator’s Manual 3. Execute the required command (see the example in Figure C-6). Figure C-7. Chromeleon 6.8 Commands Dialog Box: Example Relay Control Command Doc. 065259-05 10/12...
  • Page 123 C • Relay Control Controlling the Relay Output with an Instrument Method or Program To add relay output control commands to a Chromeleon 7 instrument method, use the Script Editor (see Figure C-1). Figure C-1. Chromeleon 7 Script Editor Doc. 065259-05 10/12...
  • Page 124 Dionex AS-DV Operator’s Manual In Chromeleon 6.8, you can enter the commands on the Relay and State Devices Options page in the Program Editor or Program Wizard (see Figure C-8). Figure C-8. Chromeleon 6.8 Program Wizard: Example Relay Output Control Command...

  • Page 125: D • Reordering Information

    Kits 068920 6-Port High-Pressure Valve Kit 068921 10-Port High-Pressure Valve Kit 060258 Dionex AS-DV and AS40 Simultaneous Injection Kit for ICS Systems 072678 Dionex AS-DV Preventive Maintenance Kit Miscellaneous Items 055075 Drain hose, convoluted, 10-mm (0.4-in) ID, 1.2 m (4 ft) long Doc.
  • Page 126 Dionex AS-DV Operator’s Manual Part Item Quantity Number 954745 Fuse, IEC 127, rated 3.15 A Doc. 065259-05 10/12...
  • Page 127 Index Numerics 10-port valve, 11 Cables See also High-pressure valves Power cord, 84 6-port valve, 11 USB, 13, 95 See also High-pressure valves Carousel Description, 8 Loading, 25 Release button, 7, 9 Removing, 70 Adapters for 0.5 mL vials, 10 Carousel Error Counter, 20 Advanced Edit Mode, 31, 34 Carousel LED, 8...
  • Page 128 Dionex AS-DV Operator’s Manual Creating a program, 19 Fitting replacement, 69 Creating an instrument method, 19 Flow schematics Concentrator mode, 30 High-pressure valves, 16 Connected LED, 8 Flush Factor, 32 Control panel Front interior, 11 AS-DV, 29, 101 Accessing, 11...
  • Page 129 Index High-pressure valve installation, 90 Power cord connection, 96 Operating features Sample out line connection, 88 Front interior, 11 Simultaneous injection setup, 102 Front view, 7 TTL and relay connectors, 108 Rear panel, 12 Unpacking the AS-DV, 87 Operating principles, 14 Instrument methods (Chromeleon), 19 High-pressure valves, 15 Creating, 30...
  • Page 130 Dionex AS-DV Operator’s Manual Power switch, 13 Manually raising sampling head, 77 USB receptacle, 13 Sampling tip replacement, 74 Rebuilding the high-pressure valve, 79 Tubing and fittings replacement, 69 Regulatory information, 3 Ship Kit, 2 Relay output, 13 Simultaneous injections, 102...
  • Page 131 Index Unexpected peaks detected, 66 Unpacking the AS-DV, 87 USB cable, 95 USB receptacle, 13 Valve Actuation Counter, 20 Valves See High-pressure valves Vial adapters for 0.5 mL vials, 10 Vial caps, 9 Cleaning, 22 Filters, 9 Insertion tool, 9 Installing, 24 Vial Process Counter, 20 Vial sensors, 8...
  • Page 132 Dionex AS-DV Operator’s Manual Index-6 Doc. 065259-05 10/12...

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