Table of Contents
5 Operation
5.6 Pressure alarms
Step
3
4
5
Pre-column pressure alarms
It is important that the pre-column pressure alarm is set during all runs where a column is used. The
pressure alarm can be set in:
• the method to be run,
• the System Settings dialog , or
• during a manual run
Pre-column pressure alarm limits are automatically set in the method when a column from the column
list is selected in the method. Refer to UNICORN Method Manual for more information on pressure
alarms.
For some columns the max delta-column pressure (media) is significantly lower than the max pre-
column pressure (hardware). To protect the media if a delta-column pressure measurement is not
available (that is, when column valve V9-C or V9H-C is not used), the pre-column pressure alarm must
be manually set to the value in the column list that is the lowest of the max pre-column pressure and
the max delta-column pressure.
Delta-column pressure alarms
If column valve V9-C or V9H-C is installed the delta-column pressure will be measured, but the alarm
must be set manually if needed.
Set pressure alarms
Pressure alarm limits may be set manually in System Control. The example below describes how to
set the high pressure limit for the column. Other alarms are set in a corresponding way.
Step
1
178
Action
• Select the inner diameter of the tubing between the injection valve and the column
from the I.D. drop-down list.
• Type in the tubing Length.
If the sample pump is used:
• Select Tubing: Sample pump to injection.
• Set tubing i.d. and length, see step 3.
Click OK.
Note:
The system now calculates the Pre-column pressure.
Action
Select Manual:Execute Manual Instructions... in the System Control module.
Result: The Manual instructions dialog opens.
ÄKTA pure User Manual 29119969 AB
Table of Contents
loading

Table of Contents