GE TYPHOON FLA 7000 Getting Started Manual
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GE TYPHOON FLA 7000 Getting Started Manual

GE TYPHOON FLA 7000 Getting Started Manual

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Getting Started with
Typhoon
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FLA 7000
Original instructions
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Summary of Contents for GE TYPHOON FLA 7000

  • Page 1 Getting Started with Typhoon ™ FLA 7000 Original instructions...

  • Page 2: Table Of Contents

    3.5.2 Installing Typhoon FLA 7000 Control Software for Windows Vista ......3.5.3 Installing 32-bit Typhoon FLA 7000 Control Software for Windows 7 ....3.5.4 Installing 64-bit Typhoon FLA 7000 control software for Windows 7 ....Preparations before starting Typhoon FLA 7000 ..............
  • Page 3 Read the sample ........................... Reading digitization samples ................Set the reading mode and reading conditions ............... Place the sample on the stage ....................... Place the stage in the Typhoon FLA 7000 ................. Read the sample ........................... Appendix A Other settings ..................

  • Page 4: Introduction

    This manual provides you with the instructions needed to handle the Typhoon FLA 7000 system in a safe way. Prerequisites In order to operate the Typhoon FLA 7000 in the way it is intended, the following prereq- uisites must be fulfilled: •...

  • Page 5: Important User Information

    Typhoon FLA 7000 is a fast laser scanner optimized for quantitative phosphorimaging, Amersham ECL Plex™ Western blots, visible fluorescence and gel documentation. Typhoon FLA 7000 is intended for research use only, and shall not be used in any clinical procedures, or for diagnostic purposes.

  • Page 6: Typographical Conventions

    Software items are identified in the text by bold italic text. A colon separates menu levels, thus File:Open refers to the Open command in the File menu. Hardware items are identified in the text by bold text (for example, Power). Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 7: Regulatory Information

    1 Introduction 1.1 Important user information Regulatory information This section lists the directives and standards that are fulfilled by the Typhoon FLA 7000 system. Manufacturing information The table below summarizes the required manufacturing information. For further infor- mation, see the EU Declaration of Conformity document.

  • Page 8: Fcc Compliance

    • connected to other products recommended or described in the user documentation, • used in the same state as it was delivered from GE, except for alterations described in the user documentation. FCC compliance This device complies with part 15 of the FCC Rules. Operation is subject to the following...

  • Page 9: Environmental Requirement

    Information Products, China Restriction of Hazardous Substances (RoHS) Regulation (EC) No Registration, Evaluation, Authorization and restriction of 1907/2006 CHemicals (REACH) Laser standards This instrument is a Class 1 (IEC60825-1:2007/EN60825-1:2007) laser product. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 10 The beam divergence of all laser modules is collimated. Regulatory compliance of connected equipment Any equipment connected to Typhoon FLA 7000 should meet the safety requirements of EN/IEC 61010-1, or relevant harmonized standards. Within EU, connected equipment must be CE marked.

  • Page 11: The Typhoon Fla 7000 Laser Scanner

    1.3 The Typhoon FLA 7000 laser scanner The Typhoon FLA 7000 laser scanner Major features The compact Typhoon FLA 7000 is an image analyzer that offers high performance, multifunctionality, and high-speed reading. Benefits include: • Detection of high-sensitivity autoradiographs using storage phosphor screens.

  • Page 12: Parts And Functions

    1.3 The Typhoon FLA 7000 laser scanner Parts and functions Part Function Power switch, located between the AC connector and the USB connector Indicator lamps Knob Setting block Filter change door Filter module slot Filter module Filter Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 13 1 Introduction 1.3 The Typhoon FLA 7000 laser scanner Part Function Phosphor Stage Suction rod Protection cover for storage phosphor screen Fluor Stage Digitizing fluorescent plate Membrane weight (included in Fluor stage) Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 14: Typhoon Fla 7000 Control Software

    Microsoft Windows 7 Professional (64-bit) Internal memory 1 GB ® Processor Intel Core 2 Duo processor Hard drive 80 GB Monitor resolution 1280 × 1024 pixels Other requirements One USB 2.0 port DVD-ROM drive Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 15: Overview Of The Main Window

    1 Introduction 1.4 Typhoon FLA 7000 control software Overview of the main window The Typhoon FLA 7000 is used to control, use and supervise the Typhoon FLA 7000. Part Function Phosphorimaging button: click to read a storage phosphor screen Fluorescence button: click to read a fluorescent sample Digitization button: click to perform digitization Method button: click to register or erase a combination of laser and filter.
  • Page 16 PMT: set the voltage of the photo-multiplier tube. The higher the value, the higher the sensitivity. Click to increase the number of scans Click to decrease the number of scans Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 17 File Size: the estimated size of the result data file is presented. Reading Time: the estimated time required for the scan is present- Top: return to the main window. Start Scan: start the scan. The sample must be loaded before starting a scan. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 18: Safety Instructions

    2 Safety instructions Safety instructions In this chapter This chapter contains the following sections: Section See page 2.1 Safety precautions 2.2 Labels 2.3 Emergency procedure 2.4 Recycling information 2.5 Declaration of Hazardous Substances (DoHS) Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 19: Safety Precautions

    Safety precautions Introduction The Typhoon FLA 7000 is powered by mains voltage and is used to image samples that may be hazardous. Before installing, operating or maintaining the equipment, you must be aware of the hazards described in the user documentation. Follow the instructions provided to avoid personal injury or damage to the equipment.

  • Page 20: Laser Safety

    WARNING Do not allow liquids, flammable materials or metallic objects to get into the Typhoon FLA 7000. This may result in fire or electric shock. Turn off the power switch, unplug the equipment from the power outlet, then contact you local GE representative.
  • Page 21 Wear gloves, protective glasses and a lab coat or similar when disposing of the Typhoon FLA 7000. Installing and moving the instrument WARNING The Typhoon FLA 7000 instrument must always be connected to a grounded power outlet. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 22 CAUTION • Do not connect any USB devices other than the Typhoon FLA 7000 to the computer in which the Typhoon FLA 7000 Control Software is installed. • Use only the Typhoon FLA 7000 Control Software during read- ing.
  • Page 23 Do not use excessive amounts of liquids for cleaning the Typhoon FLA 7000, this may result in product malfunction or electric shock. CAUTION Wear gloves to prevent direct contact with chemical substances. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 24 Turn off the power switch and remove connecting cables before moving the equipment. CAUTION Turn the power switch off before cleaning the inside of the equip- ment. CAUTION Unplug the equipment if it will not be used for an extended period. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 25: Labels

    2 Safety instructions 2.2 Labels Labels Typhoon FLA 7000 serial number The Typhoon FLA 7000 serial number is located on a label on the back of the instrument. Symbols used in safety labels Label Meaning Warning! Read the user documentation before using the system. Do not open any covers or replace parts unless specifically stated in the user documentation.
  • Page 26 Exposure to laser light can cause injury. Viewing the laser light directly can cause blindness. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 27 2 Safety instructions 2.2 Labels Labels at the front of Typhoon FLA 7000 Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 28 2 Safety instructions 2.2 Labels Labels at rear of Typhoon FLA 7000 Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 29 2 Safety instructions 2.2 Labels Labels on left side of Typhoon FLA 7000 I : Power ON O : Power OFF Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 30: Emergency Procedure

    2 Safety instructions 2.3 Emergency procedure Emergency procedure This section describes how to do an emergency shutdown of the Typhoon FLA 7000 in- strument. The section also describes the results of a power failure. Emergency shutdown Step Action Click the Stop button in the Typhoon FLA 7000.

  • Page 31: Recycling Information

    2.4 Recycling information Recycling information General instructions for disposal When taking the Typhoon FLA 7000 out of service, the different materials must be sepa- rated and recycled according to national and local environmental regulations. Specific instructions for disposal Measure the superficial radio isotope pollution of the instrument body and storage phosphor screen as mentioned in the radiation hazard prevention section of the User Manual.

  • Page 32: Declaration Of Hazardous Substances (Dohs)

    EFUP shall be done in accordance with the Product Maintenance Procedures. This product must not be disposed of as unsorted municipal waste, and must be collected separately and handled properly after decommissioning. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 33 Indicates that this toxic or hazardous substance contained in at least one of the homoge- neous materials used for this part is above the limit requirement in SJ/T11363-2006. Data listed in the table represents best information available at the time of publication. • Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 34: Installation

    This chapter contains the following sections: Section See page 3.1 Site requirements 3.2 Transporting Typhoon FLA 7000 3.3 Delivery inspection 3.4 Connections 3.5 Software installation 3.6 Preparations before starting Typhoon FLA 7000 Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 35: Site Requirements

    3 Installation 3.1 Site requirements Site requirements Note: The Typhoon FLA 7000 is intended for indoor use only. Parameter Requirement Power supply 100 to 240 V AC ±10%, 3.0 to 1.5 A Line frequency 50 to 60 Hz Placement Stable, horizontal surface.

  • Page 36: Transporting Typhoon Fla 7000

    Typhoon FLA 7000 must be secured before long distance transports. Contact GE for help and advice before transporting Typhoon FLA 7000 long distances. The Typhoon FLA 7000 weighs approximately 62 kg and requires at least two persons to lift and move. Precautions before moving the...

  • Page 37: Delivery Inspection

    Delivery inspection What to check Upon receiving Typhoon FLA 7000, inspect the package for external damages. Should you find any external damages, notify the delivery company and contact GE for further advice. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 38: Connections

    Use only the Typhoon FLA 7000 Control Software during read- ing. Electrical power Connect the power cord of the Typhoon FLA 7000 to a grounded power outlet. WARNING Use only power cords delivered or approved by GE. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 39: Software Installation

    Installation sequence Software installation is performed in the following sequence: 1 Install the USB control driver. 2 Install the USB function driver (Windows XP only). 3 Install theTyphoon FLA 7000 control software. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 40: Installing Typhoon Fla 7000 Control Software For Windows Xp

    3 Installation 3.5 Software installation 3.5.1 Installing Typhoon FLA 7000 Control Software for Windows XP 3.5.1 Installing Typhoon FLA 7000 Control Software for Windows XP Before you begin Log in using a Windows account with administrator privileges. Install the USB Control Driver for...
  • Page 41 3.5.1 Installing Typhoon FLA 7000 Control Software for Windows XP Step Action Insert the Typhoon FLA 7000 DVD and click the Browse button. Select to install the driver from the Typhoon FLA 7000 DVD. Open the USB Control folder. Select the DevMng.inf file and click the Open button.
  • Page 42 Windows XP Step Action Connect the computer and the Typhoon FLA 7000 with a USB cable and turn the power switch of the Typhoon FLA 7000 to ON. The scanner is automati- cally detected by the computer. In the Found New Hardware Wizard dialog, choose No, not this time.
  • Page 43 Action Read the license text. If the license agreement is not acceptable please contact a GE representative, see back cover of this manual for contact infor- mation. Select I accept the terms in the license agreement and click the Next button.

  • Page 44: Installing Typhoon Fla 7000 Control Software For Windows Vista

    Select Install the hardware that I manually select from a list (Advanced) and click the Next button. Select Show All Devices and click the Next button. Click the Have Disk button. Insert the Typhoon FLA 7000 DVD and click the Browse button. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 45 3.5.2 Installing Typhoon FLA 7000 Control Software for Windows Vista Step Action Select to install the driver from the Typhoon FLA 7000 DVD. Select the USB Control folder and click Open. Select the file DevMng.inf and click the Open button.
  • Page 46 Insert the Typhoon FLA 7000 DVD. Locate and double-click the file Typhoon FLA 7000.exe. In the Typhoon FLA 7000 - InstallShield Wizard dialog, click the Next button. Read the license text. If the license agreement is not acceptable, please contact a GE representative. See the back cover of this manual for contact information.
  • Page 47 Click Install this driver software anyway in the Windows Security dialog. Click the Finish button. The installation of Typhoon FLA 7000 is now completed. Connect the computer and the Typhoon FLA 7000 with the USB cable. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 48: Installing 32-Bit Typhoon Fla 7000 Control Software For Windows 7

    3 Installation 3.5 Software installation 3.5.3 Installing 32-bit Typhoon FLA 7000 Control Software for Windows 7 3.5.3 Installing 32-bit Typhoon FLA 7000 Control Software for Windows 7 Disable the computer sleep function Log in using a Windows administrator account. Disable the computer sleep function as described below.
  • Page 49 3 Installation 3.5 Software installation 3.5.3 Installing 32-bit Typhoon FLA 7000 Control Software for Windows 7 Identify the operating system Two software installers are included in this software. To choose the appropriate installer find out which operating system the computer is using by following the instructions below.
  • Page 50 If the installer does not match with the type of the computer operating system, the Typhoon FLA 7000 is not installed successfully. If one of the following messages is dis- played during the installation process, follow the instructions below.
  • Page 51 3 Installation 3.5 Software installation 3.5.3 Installing 32-bit Typhoon FLA 7000 Control Software for Windows 7 Cause Correction The computer is running on Windows 7 with Restart the installation using the 32-bit operating system. You have chosen installer for Windows 7 with 32-bit the installer for Windows 7 with 64-bit oper- operating system.
  • Page 52 3 Installation 3.5 Software installation 3.5.3 Installing 32-bit Typhoon FLA 7000 Control Software for Windows 7 Step Action In the details pane, select Action:Add legacy hardware. In the Add Hardware dialog, click Next. Select Install the hardware that I manually select from a list (Advanced) and click Next.
  • Page 53 3 Installation 3.5 Software installation 3.5.3 Installing 32-bit Typhoon FLA 7000 Control Software for Windows 7 Step Action Select Show All Devices and click Next. Click Have Disk. Insert the Typhoon FLA 7000 DVD and click Browse. Select to install the driver from the Typhoon FLA 7000 DVD.
  • Page 54 3 Installation 3.5 Software installation 3.5.3 Installing 32-bit Typhoon FLA 7000 Control Software for Windows 7 Step Action Select the file DevMng.inf and click Open. Click OK in the Install from disk dialog. Click Next in the Add Hardware wizard.
  • Page 55 Continue to proceed with the installation. Step Action Insert the Typhoon FLA 7000 DVD. Locate and double-click the file Typhoon FLA 7000.exe. In the Typhoon FLA 7000 - InstallShield Wizard dialog, click the Next button. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 56 Action Read the license text. If the license agreement is not acceptable, please contact a GE representative. See the back cover of this manual for contact information. Select I accept the terms in the license agreement and click the Next button.
  • Page 57 Allow. Click Install this driver software anyway in the Windows Security dialog. Click the Finish button to complete the installation. Connect the computer and the Typhoon FLA 7000 with the USB cable. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 58: Installing 64-Bit Typhoon Fla 7000 Control Software For Windows 7

    3 Installation 3.5 Software installation 3.5.4 Installing 64-bit Typhoon FLA 7000 control software for Windows 7 3.5.4 Installing 64-bit Typhoon FLA 7000 control software for Windows 7 Disable the computer sleep function Log in using a Windows administrator account. Disable the computer sleep function as described below.
  • Page 59 If the installer does not match with the type of the computer operating system, the Typhoon FLA 7000 is not installed successfully. If one of the following messages is dis- played during the installation process, follow the instructions below.
  • Page 60 3 Installation 3.5 Software installation 3.5.4 Installing 64-bit Typhoon FLA 7000 control software for Windows 7 Cause Correction The computer is running on Windows Uninstall Typhoon FLA 7000 for Win- 7 with 64-bit operating system. You dows 7 with 32-bit operating system.
  • Page 61 3 Installation 3.5 Software installation 3.5.4 Installing 64-bit Typhoon FLA 7000 control software for Windows 7 Step Action Click Control Panel:Hardware and Sound, then click Device Manager under Devices and Printers. On the Action menu, click Add legacy hardware. In the Add Hardware dialog, click Next.
  • Page 62 3 Installation 3.5 Software installation 3.5.4 Installing 64-bit Typhoon FLA 7000 control software for Windows 7 Step Action Select Install the hardware that I manually select from a list (Advanced) and click Next. Select Show All Devices and click Next.
  • Page 63 Action Insert the Typhoon FLA 7000 DVD and click Browse. In the Look in list, click Computer (1), then click Typhoon FLA 7000 control software DVD (2). Click Open (3). In the Installer for 64bit:USB Control 64 folder, click Open.
  • Page 64 3 Installation 3.5 Software installation 3.5.4 Installing 64-bit Typhoon FLA 7000 control software for Windows 7 Step Action In Add Hardware wizard, click Next. When the message The wizard is ready to install your hardware is dis- played, click Next.
  • Page 65 3 Installation 3.5 Software installation 3.5.4 Installing 64-bit Typhoon FLA 7000 control software for Windows 7 Step Action In Add Hardware wizard, click Finish. Install Typhoon FLA 7000 and USB function driver Note: During software installation you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue.
  • Page 66 3 Installation 3.5 Software installation 3.5.4 Installing 64-bit Typhoon FLA 7000 control software for Windows 7 Step Action Read the license agreement text. If the license agreement is not acceptable, please contact your GE representative. Contact information can be found on the back page of this document.
  • Page 67 3 Installation 3.5 Software installation 3.5.4 Installing 64-bit Typhoon FLA 7000 control software for Windows 7 Step Action Click Install. If User Account Control (UAS) is enabled, a dialog displays the message An unidentified programs wants access to your computer. Click Allow.
  • Page 68 3 Installation 3.5 Software installation 3.5.4 Installing 64-bit Typhoon FLA 7000 control software for Windows 7 Step Action In Typhoon FLA 7000 - InstallShield Wizard, click Finish. Uninstall the software Follow the instructions below to uninstall the Typhoon FLA 7000.
  • Page 69 Upgrade the software Follow the instructions below to upgrade the Typhoon FLA 7000. Step Action Uninstall the current version of the Typhoon FLA 7000 as described in the section Uninstall the software, on page 68 above. Install the new version of the Typhoon FLA 7000 as described above.

  • Page 70: Preparations Before Starting Typhoon Fla 7000

    The air exhaust fan on the rear side of Typhoon FLA 7000 is unobstructed. • Typhoon FLA 7000 is not placed in direct sunlight or in a brightly lit place. • There are no objects on top of Typhoon FLA 7000.

  • Page 71: Operation

    4 Operation Operation In this chapter This chapter contains the following sections: Section See page 4.1 Operation overview 4.2 Starting the instrument and the Typhoon FLA 7000 control soft- ware Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 72: Operation Overview

    4 Operation 4.1 Operation overview Operation overview Using Typhoon FLA 7000 comprises a series of steps outlined below. Detailed explanations are provided in subsequent chapters. Turn on the Typhoon FLA 7000 Phosphorimaging samples Start the computer and launch the Typhoon FLA 7000...

  • Page 73: Starting The Instrument And The Typhoon Fla 7000 Control Software

    4 Operation 4.2 Starting the instrument and the Typhoon FLA 7000 control software Starting the instrument and the Typhoon FLA 7000 control software Note: Before turning on the instrument, open the lid and make sure that the stage rack is empty. Then, close the lid securely.
  • Page 74 Typhoon FLA 7000 is lit. Start Typhoon FLA 7000 control software from the Start menu, or use the desktop shortcut. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 75 4.2 Starting the instrument and the Typhoon FLA 7000 control software Step Action The main window of the Typhoon FLA 7000 control software is displayed. The condition is displayed in the Status area of the main window. Status messages are as follows:...

  • Page 76: Reading Fluorescent Samples

    This chapter contains the following sections: Section See page 5.1 Set the reading mode and reading conditions 5.2 Place the sample on the stage 5.3 Place the stage in Typhoon FLA 7000 5.4 Read the sample Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 77: Set The Reading Mode And Reading Conditions

    5.1 Set the reading mode and reading conditions Set the reading mode and reading conditions Start the system 1 Turn on Typhoon FLA 7000 and the computer. 2 Start the Typhoon FLA 7000 control software. Set the reading conditions Click the Fluorescence button in the main window to reach the Reader Settings window for the fluorescence mode .
  • Page 78 For details, refer to the User Manual. Load Condition... click this button to load previously saved reading conditions, if desired. For details, refer to the User Manual. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 79: Place The Sample On The Stage

    The sample must not be thicker than 30 mm. For solutions, the maximum height is 4 mm. • Make sure there are no bubbles or gaps on the contact surface. Step Action Place the sample on the Fluor stage. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 80 Place the titer plate in the desired position in the titer plate frame. Placing a gel sample with glass on the Multi stage Step Action Place a gel sample with glass on the Multi stage. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 81 5 Reading fluorescent samples 5.2 Place the sample on the stage Step Action Carefully fold down the spring-loaded glass holders on the glass. Tighten the screws on the glass holders as necessary. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 82: Place The Stage In Typhoon Fla 7000

    5 Reading fluorescent samples 5.3 Place the stage in Typhoon FLA 7000 Place the stage in Typhoon FLA 7000 Placing the Fluor or Multi stage in the Typhoon FLA 7000 Step Action Pull the knob up and open the lid.

  • Page 83: Read The Sample

    Adjust the gradations in the image. Exponential Exponential gradation adjustment Linear Linear gradation adjustment Sigmoid Sigmoid gradation adjustment Magnification Zoom in or out. Auto range scope Check this option to optimize the tones automatically. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 84: Exposing Storage Phosphor Screens

    6 Exposing Storage phosphor screens Exposing Storage phosphor screens In this chapter This chapter contains the following sections: Section See page 6.1 Handling precautions 6.2 Preparing the Storage phosphor screen 6.3 Exposing the Storage phosphor screen Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 85: Handling Precautions

    Prying the Storage phosphor out may result in the edge peeling off, making the Storage phosphor screen unusable. NOTICE • Do not scratch or bend the Storage phosphor screen. • Keep the Storage phosphor screen free from dust. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 86 Storage NOTICE Remove the Storage phosphor screen from the Typhoon FLA 7000 main unit when not in use. Store the Storage phosphor screens in a horizontal position, protected from moisture. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 87: Preparing The Storage Phosphor Screen

    Dim the ambient light to 20 lux or less before opening or moving the Storage phosphor screen without a cover. • Protect the phosphor stage with the protective cover when removing an exposed Storage phosphor screen from the cassette and placing it on the Phosphor stage. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 88 X-ray film. Take this into consideration when setting the initial exposure time. Tip: For increased image quality, minimize the time between exposing the Storage phosphor screen and reading it with the Typhoon FLA 7000. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 89: Exposing The Storage Phosphor Screen

    Place the sample on the cassette. Make sure that the sample surface faces Note: Keep the radio imaging sample away from the edge of the Storage phosphor screen. Otherwise, the recorded data may become corrupted. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 90 Storage phosphor screen is in the front left corner of the cassette as shown below. Close the cover of the cassette. Press on the cover until it clicks into place. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 91: Reading Phosphorimaging Samples

    Section See page 7.1 Set the reading conditions 7.2 Place the storage phosphor screen on the phosphor stage 7.3 Place the phosphor stage in the Typhoon FLA 7000 7.4 Read the sample Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 92: Set The Reading Conditions

    7.1 Set the reading conditions Set the reading conditions Start the system 1 Turn on Typhoon FLA 7000 and the computer. 2 Start the Typhoon FLA 7000 control software. Set the reading conditions Click the Phosphorimaging button to reach the Reader Settings window for the phos- phorimaging mode.
  • Page 93 Select All to use the entire Fluor stage as the reading area. • Save Condition... click this button to save the current reading conditions in a file, if desired. For details, refer to the User Manual. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 94 7 Reading phosphorimaging samples 7.1 Set the reading conditions Part Description Load Condition... click this button to load previously saved reading conditions, if desired. For details, refer to the User Manual. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 95: Place The Storage Phosphor Screen On The Phosphor Stage

    Pick up the storage phosphor screen and move it to the phosphor stage. Place the storage phosphor screen on the back of the phosphor stage, with the white or blue reading surface of the storage phosphor screen facing up. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 96: Place The Phosphor Stage In The Typhoon Fla 7000

    7 Reading phosphorimaging samples 7.3 Place the phosphor stage in the Typhoon FLA 7000 Place the phosphor stage in the Typhoon FLA 7000 Step Action Pull the knob up and open the lid. Position the stage so that the black triangle mark is aligned with the corre- sponding mark on the setting block.

  • Page 97: Read The Sample

    Adjust the gradations in the image. Exponential Exponential gradation adjustment Linear Linear gradation adjustment Sigmoid Sigmoid gradation adjustment Magnification Zoom in or out. Auto range scope Check this option to optimize the tones automatically. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 98: Reading Digitization Samples

    This chapter contains the following sections: Section See page 8.1 Set the reading mode and reading conditions 8.2 Place the sample on the stage 8.3 Place the stage in the Typhoon FLA 7000 8.4 Read the sample Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 99: Set The Reading Mode And Reading Conditions

    8.1 Set the reading mode and reading conditions Set the reading mode and reading conditions Start the system 1 Turn on Typhoon FLA 7000 and the computer. 2 Start the Typhoon FLA 7000 control software. Set the reading conditions Click the Digitization button of the main window to reach the Reader Settings window for the digitization mode.
  • Page 100 For details, refer to the User Manual. Load Condition... click this button to load previously saved reading conditions, if desired. For details, refer to the User Manual. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 101: Place The Sample On The Stage

    Make sure there are no bubbles or gaps on the contact surface. Step Action Place the sample on the Fluor stage. Place the fluorescent plate for digitization on top of the sample. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 102: Place The Stage In The Typhoon Fla 7000

    8 Reading digitization samples 8.3 Place the stage in the Typhoon FLA 7000 Place the stage in the Typhoon FLA 7000 Step Action Pull the knob up and open the lid. Position the stage so that the black triangle mark is aligned with the corre- sponding mark on the setting block.

  • Page 103: Read The Sample

    Adjust the gradations in the image. Exponential Exponential gradation adjustment Linear Linear gradation adjustment Sigmoid Sigmoid gradation adjustment Magnification Zoom in or out. Auto range scope Check this option to optimize the tones automatically. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 104: Appendix A Other Settings

    Note: The correction mode settings list is registered by a serviceman. Please contact the dealer where you purchased Typhoon FLA 7000, or contact ND Filter Enables the use of an ND filter to adjust the light intensity. Use the ND filter if the signal from the sample is very strong.
  • Page 105 User account specific settings Changes to the settings in the Preferences dialog affect only the current Windows user account. Changes made using a different user account do not affect the current user account. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 106: Appendix B Daily Maintenance

    Wipe the stage with a sponge moistened with a fluorescence-free neutral detergent. Thoroughly rinse the stage with water and dry with a lint-free cloth. Place the stage in the main body of the instrument. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 107: Appendix C Periodic Maintenance

    If you use the SHG laser in the Typhoon FLA 7000, it will require periodical calibration. When the Typhoon FLA 7000 is switched on, it automatically executes calibration. You do not need to do a manual calibration if the Typhoon FLA 7000 is used at least once a month.

  • Page 108: Appendix D Installing And Replacing Filters

    2 If replacing a filter, remove the filter to be replaced from the filter module. 3 Install the new filter in the filter module. 4 Place the filter module in the scanner. 5 Register the filter change in the Typhoon FLA 7000. Remove the filter module from the scanner...
  • Page 109 Open the filter change door. Pull filter module straight out. Remove and install filters in the filter module Step Action Press the notch on the bottom of the filter module and pull out the filter. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 110 Place the new filter in the filter module. Push the new filter in until it locks in place. Place the filter module in the scanner Step Action Open the filter change door. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 111 Close the filter change door. Register the filter change in Typhoon FLA 7000 Step Action Click the Filter Module button in the main window. Click the desired filter position on the filter tray. Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 112 Select the filter to be registered from Filter List. Click the Insert button to register the filter. Tip: You can also click and drag the filter from Filter List to the desired filter posi- tion. Click the OK button. Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 113: Appendix E Default Sample Detection Methods

    Y520 Alexa Fluor™ 488 Y520 Digitize473 Y520 532 nm laser Method name Filter type O580 EtBR O580 ROX™ O580 Pro-Q™ Diamond O580 Digitize532 O580 635 nm laser Method name Filter type R670 Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 114 E Default sample detection methods Method name Filter type Alexa Fluor 633 R670 DDAO R670 650 nm laser Method name Filter type Phosphorimaging R710 Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 115: Appendix F Specifications

    24 cm x 40 cm maximum (22 cm x 36 cm valid field angle) (238 mm x 398 mm setting size) Parameter Data Pixel size 25, 50, 100 or 200 µm (selectable) Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 116: Power Supply

    The Reading block is a standard unit. The other units are optional. Power supply Parameter Data Input voltage 100 to 240 V~ (AC) Allowable variations in ±10% voltage Frequency 50 to 60 Hz Rated current 1.5 to 3.0 A Getting Started with Typhoon FLA 7000 28-9607-64 AD...

  • Page 117: Environmental Conditions

    2,000 m above sea level Overvoltage category Transient overvoltage category II Applicable Pollution Pollution Degree 2 Rating Noise levels Parameter Data Noise 70 dB (A) or lower Degrees of protection IP20 provided by enclosure Getting Started with Typhoon FLA 7000 28-9607-64 AD...
  • Page 118 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare repre- sentative for the most current information.

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