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Thermo Scientific Evolution 260 Bio Manuals
Manuals and User Guides for Thermo Scientific Evolution 260 Bio. We have
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Thermo Scientific Evolution 260 Bio manual available for free PDF download: User Manual
Thermo Scientific Evolution 260 Bio User Manual (500 pages)
UV-Vis Instruments
Brand:
Thermo Scientific
| Category:
Laboratory Equipment
| Size: 8.38 MB
Table of Contents
Table of Contents
3
About the Software and Hardware
15
Introduction
15
Overview
15
About this Document
16
Before Using the Spectrophotometer
16
Setting up the Instrument
16
Contacting Us
17
E-Mailing Data Files
18
Product Warranty
18
Items Not Covered by Warranty
19
Warranty Period
19
Warranty Repair
19
Shipping Cost for Items Covered by Warranty
20
Trademarks
20
Update Policy
20
Site Preparation and Safety
21
Applications
23
Fixed
23
INSIGHT Software
23
Quant
24
Rate
24
Scan
24
The INSIGHT Window
25
Action Buttons
26
Navigation Pane
26
Other Navigation Pane Features
26
Task Buttons
26
Right Pane
28
Data Display
29
Menus
35
Instrument Status Monitors
37
Measuring Samples
38
Operation
38
Thermo Software IQ
38
Performing a Quantitative Analysis
39
Quantifying Sample Data
40
Performing a Kinetics Measurement
45
Performing Kinetics Measurements
46
Using the Palette Tools and View Finder
50
Using the Selection Tool
50
Using the Spectral Cursor Tool
50
Using the Peak Area Tool
51
Using the Peak/Valley Measurement Tool
51
Using the Region-Threshold Tool
51
Using the View Finder
51
Setting Application Parameters
55
Fixed
56
Scan
60
Quant
65
Rate
82
Sharing Custom Templates
87
Managing Data
88
My Data
88
Reports
89
Adding Two Scan Spectra
91
Converting Scan Data to a Derivative
91
Converting Scan Data to Other Units
91
Math and Analysis Operations
91
Normalizing Scan Data
91
Ratioing Two Scan Spectra
91
Smoothing Scan Data
91
Subtracting a Scan Spectrum
91
Averaging Scan Spectra
102
Adding, Subtracting, Multiplying or Dividing Scan Data by a Factor
103
Finding Peaks in Scan Data
105
Finding Value Level Crossings in Scan Data
107
Applications Tab
110
Options
110
Report Master Page Tab
110
Data Store Tab
111
Preferences Tab
111
Formulas and Units Tab
112
Alignment Tab
113
System Settings
113
Calibrations Tab
114
Keyboard Shortcuts
115
Lamp Tab
115
System Tab
115
Evolution 200 Series Spectrophotometers
117
Safety Considerations
117
Safety Label Locations
118
Lifting or Moving the Instrument
120
Operating Precautions
120
Local Control or Computer Control
121
Opening and Closing the Sample and Reference Compartment Doors
121
Spectrophotometer Basics
121
Connectors
122
INSIGHT Software
122
Keypad
122
Touchscreen
122
Inside the Sample Compartment
123
Outside Cover
123
Removable Panels
125
Sample Compartment Front Panel
125
Tubing Access Panel
126
Mercury Lamp Access Cover
127
Detector Access Cover
128
Corrosion Protection
130
Detector Access Panel
130
Installing Sample Compartment Windows
130
Purging the Sample Compartment
130
10 MM Rectangular Sample Cell Holder
132
Standard Cell Holders
132
Rectangular Reference Cell Holder
133
Installing a Manual Accessory
134
Installing a Smart Accessory
135
Installing a Liquid Thermostatted Accessory
138
Computer Control Instruments
140
Installing a Reference Cell Holder
140
Using the Spectrophotometer
140
Setting up a Computer Control Instrument
141
Turning off a Computer Control Instrument
141
Local Control Instruments
142
Setting up a Local Control Instrument for Touchscreen Operation
142
Setting up a Local Control Instrument for Remote Operation
143
Turning off a Local Control Instrument
143
Keypad Operation
144
Cleaning the Instrument
145
Keypad Shortcuts
145
Maintenance
145
Routine Maintenance
145
Cleaning the Touchscreen
146
Removing and Installing the Detector Module
146
Ordering Parts
149
About the Life Science Applications
151
Life Science Applications
151
Nucleic Acid
152
Nucleic Acid Concentration Calculations
153
Overview
153
Accessories Tab for Nucleic Acid
154
Application Settings
154
Instrument Tab for Nucleic Acid
154
Measurement Tab for Nucleic Acid
154
Samples Tab for Nucleic Acid
154
Type Tab for Nucleic Acid
154
Unique Screen Features
159
Making Nucleic Acid Measurements
160
Oligo Calculator
162
Nucleic Acid Labels
163
Dye/Chromophore Editor
164
Overview
164
Application Settings
165
Instrument Tab for Nucleic Acid Labels
165
Measurement Tab for Nucleic Acid Labels
165
Type Tab for Nucleic Acid Labels
165
Accessories Tab for Nucleic Acid Labels
169
Samples Tab for Nucleic Acid Labels
169
Unique Screen Features
170
Making Nucleic Acid Labels Measurements
171
Oligo Calculator
173
Overview
174
Protein A280
174
Accessories Tab for Protein A280
175
Application Settings
175
Instrument Tab for Protein A280
175
Measurement Tab for Protein A280
175
Samples Tab for Protein A280
175
Type Tab for Protein A280
175
Making Protein A280 Measurements
181
Unique Screen Features
181
Proteins and Labels
183
Application Settings
184
Dye/Chromophore Editor
184
Instrument Tab for Proteins and Labels
184
Measurement Tab for Proteins and Labels
184
Overview
184
Type Tab for Proteins and Labels
184
Accessories Tab for Proteins and Labels
189
Samples Tab for Proteins and Labels
189
Making Proteins and Labels Measurements
190
Unique Screen Features
190
Pierce BCA
192
BCA Kits and Protocols
193
Overview
193
Application Settings
194
Measurement Tab for Pierce BCA
194
Type Tab for Pierce BCA
194
Standards Tab for Pierce BCA
197
Instrument Tab for Pierce BCA
198
Accessories Tab for Pierce BCA
199
Samples Tab for Pierce BCA
199
Making Pierce BCA Measurements
200
Working with Standard Curves
200
Accessories Tab for Protein Bradford
205
Application Settings
205
Bradford Reagent Kits and Protocols
205
Instrument Tab for Protein Bradford
205
Measurement Tab for Protein Bradford
205
Overview
205
Protein Bradford
205
Samples Tab for Protein Bradford
205
Standards Tab for Protein Bradford
205
Type Tab for Protein Bradford
205
Bradford Standard Curves
211
Making Protein Bradford Assay Measurements
211
Pierce Modified Lowry
215
Accessories Tab for Pierce Modified Lowry
216
Application Settings
216
Instrument Tab for Pierce Modified Lowry
216
Measurement Tab for Pierce Modified Lowry
216
Modified Lowry Kits and Protocols
216
Overview
216
Samples Tab for Pierce Modified Lowry
216
Type Tab for Pierce Modified Lowry
216
Lowry Standard Curves
222
Making Pierce Modified Lowry Assay Measurements
222
Pierce 660 Nm Protein Assay
226
Application Settings
227
Overview
227
Pierce 660 Nm Reagent and Protocols
227
Type Tab for Pierce 660 Nm Protein Assay
227
Measurement Tab for Pierce 660 Nm Protein Assay
228
Standards Tab for Pierce 660 Nm Protein Assay
230
Instrument Tab for Pierce 660 Nm Protein Assay
231
Accessories Tab for Pierce 660 Nm Protein Assay
232
Samples Tab for Pierce 660 Nm Protein Assay
232
Pierce 660 Nm Assay Workbooks
233
Standard Curves
233
Making Pierce 660 Nm Protein Assay Measurements
234
Protein Biuret
237
Accessories Tab for Protein Biuret
238
Application Settings
238
Instrument Tab for Protein Biuret
238
Measurement Tab for Protein Biuret
238
Overview
238
Samples Tab for Protein Biuret
238
Standards Tab for Protein Biuret
238
Type Tab for Protein Biuret
238
Making Protein Biuret Assay Measurements
244
Standard Curves
244
Accessories
249
Degree Near Normal Specular Reflectance
251
15 Degree Specular Reflectance
253
30 Degree Specular Reflectance
257
45 Degree Specular Reflectance
259
60 Degree Specular Reflectance
261
Calibrated Validation Carousel (CVC)
263
Installing Complete CVC in the Instrument
264
Installing the Carousel on the Smart Base Unit
264
Initialization
265
Loading CVC Calibration Data
265
CVC Test Limits
267
Running Performance Verification Tests
267
Using the CVC
267
CVC Test Results
269
Viewing CVC Test Reports
269
Maintenance
271
Routine Maintenance
271
Recertification
272
Depolarizer
273
Grazing Angle Specular Reflectance
275
Integrated Fiber Optic Module
277
Installation
278
Attaching Cables and Probes
280
Maintenance
281
Routine Maintenance
281
Aligning the Accessory
282
Troubleshooting
284
Integrating Sphere Diffuse Reflectance
285
Mercury Lamp
287
Installation and Operation
289
Operating Precautions
289
Getting Started
293
Nanocell
293
Overview
295
Introduction to Small Volume Measurements
296
Installing the Nanocell
297
Loading a Sample
297
Using the Nanocell
297
Measurements
300
Cleaning the Nanocell
301
Storing the Nanocell
301
Concentration
302
Direct UV Assays for DNA, RNA, Oligonucleotides and Protein Concentration
302
Pathlength Correction Factors
302
Frequently Asked Questions
303
TPS-1500W Sealed Peltier Recirculator
305
General Safety Information
306
Front and Back Panel
307
Product Description
307
Specifications
308
Preinstallation
311
Verifying the Power Supply and Fuse
311
Changing the Voltage Setting or Fuse
312
Installation
313
Operation
315
Cleaning the Accessory
320
Maintenance
320
Routine Maintenance
320
Filling the Reservoir
321
Acoustic Alarm
322
Other System Failures
324
Water Level Failure
324
Peltier Thermostatted Single Cell Holder
325
General Safety Information
326
Product Description
327
Temperature Controller Front and Rear Panels
327
Specifications
328
Preinstallation
330
Changing the Voltage Setting or Fuse
331
Verifying the Power Supply and Fuse
331
Installation
333
Operation
335
Setting the Target Temperature and Stir Speed
336
Using INSIGHT Software to Set Temperature and Stir Speed
336
Using the Peltier Controller to Set Temperature and Stir Speed
336
Cleaning the Accessory
342
Routine Maintenance
342
Troubleshooting
343
Probe Handling and Maintenance
345
Installation
346
Operation
347
Maintenance
348
Routine Maintenance
348
Rapid MIX Accessory
351
Cylindrical Reference Cell Holder
353
Rectangular Reference Cell Holder
353
Reference Cell Holders
353
Sample Position Cell Holders
355
Water Thermostatted 10 MM Rectangular Cell Holder
356
Cell Holder for 15 MM Beam Height Cells
357
Cylindrical Cell Holder
358
Solid Sample Holders
359
Rotatable Test Tube and Cell Holder
361
Universal Sample Holder Slide
361
Sample Holder for 1-Inch Square Cells
362
Sample Holder for Small Test Tubes and Vials
363
Variable Pathlength Rectangular Cell Holder
364
Variable Pathlength Cell Holder Slide
365
Installation
368
Installing a Smart Cell Changer
368
Operation
368
Replacing the Cell Holder
368
Smart Linear 8-Cell Changer
368
Calibrating the Cell Changer
370
Cell Changer Settings
370
Displaying the Cell Changer Status Monitor
370
Cleaning the Accessory
378
Routine Maintenance
378
Troubleshooting
379
SPE-8W Smart Peltier Linear Cell Changer
381
General Safety Information
382
Product Description
383
Temperature Controller Front and Rear Panel
383
Changing the Voltage Setting or Fuse
388
Preinstallation
388
Verifying the Power Supply and Fuse
388
Installation
390
Calibrating the Cell Changer
393
Initializing the Cell Changer
393
Operation
393
Configuring the Cell Changer
396
Setting the Target Temperature and Stir Speed
396
Measuring Samples with the Cell Changer
404
Cleaning the Accessory
406
Maintenance
406
Routine Maintenance
406
Troubleshooting
408
Smart Rotary Cell Changer
409
Installation
410
Installing a Smart Cell Changer
410
Operating Precautions
410
Replacing the Cell Holder
410
Safety Considerations
410
Cell Changer Settings
413
Displaying the Cell Changer Status Monitor
413
Initializing the Cell Changer
413
Measuring Samples with a Cell Changer
413
Measuring Standards with a Cell Changer
413
Operation
413
Cleaning the Accessory
419
Maintenance
419
Routine Maintenance
419
Safety Considerations
421
Smart Sipper
421
Installation
423
Installing a Flow Cell
423
Installing Pump Tubing
423
Installing the Smart Sipper
423
Operating Precautions
423
Calibration
429
Operation
432
Cleaning Flow Cells
434
Cleaning the Accessory
434
Maintenance
434
Replacing Flow Cell Tubing
434
Replacing Pump Tubing
434
Routine Maintenance
434
Pump Tubing Chemical Compatibility
436
Specifications
436
Troubleshooting
442
Operating Precautions
445
Temperature Probe
445
Installation
446
Operation
447
Configuring a Target Temperature
448
Displaying a Temperature Status Monitor
448
Temperature Settings
448
Cleaning Temperature Probes
451
Maintenance
451
Replacing Stopper O-Rings
451
Routine Maintenance
451
Troubleshooting
454
Variable Angle Transmission
455
Fiber Optic Probes
457
Operating Precautions
457
VERSA Probe
457
Fiber Optic Cables
458
Installation
458
Installing the VERSA Probe
458
Installing Tips
458
Operation
460
Routine Maintenance
463
Chapter 6 Performance Verification
465
Overview of PV
465
PV Test Descriptions
466
Running PV Tests
468
Chapter 7 INSIGHT Security Software
471
Signing a File
472
Verifying a Signature
473
Access Control and System Policies
473
Assigning Signature Meanings
477
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