Emission Filters - GE Typhoon 9200 Operating Instructions Manual

Appendix A

Accessories

A.3

A.3 Emission filters

The Typhoon instrument contains a standard set of emission filters and can

accommodate a total of 14 emission filters. The instrument uses emission filters to

reject reflected and scattered excitation light and background fluorescence while

allowing the emitted light from the sample to pass through to the PMT. Table A-2 lists

the standard set of emission filters used with fluorescence scanning.

Table A-2. Standard set of emission filters.

Emission filter

520-nm band-pass filter

(520 BP 40)

(Typhoon 9400 and

9410 only)

555-nm band-pass filter

(555 BP 20)

580-nm band-pass filter

(580 BP 30)

610-nm band-pass filter

(610 BP 30)

670-nm band-pass filter

(670 BP 30)

526-nm short-pass filter

560-nm long-pass filter

390-nm band-pass filter

72

Description

Transmits light between 500 nm and 540 nm and has a

transmission peak centered at 520 nm.

Use this filter with fluorochromes, such as Cy2 and ECL

Plus™, or with fluorescein when scanning with the 488-

nm wavelength of the blue laser.

Transmits light between 545 nm and 565 nm and has a

transmission peak centred at 555 nm.

Use this filter with fluorochromes, such as R6G and

HEX.

Transmits light between 565 nm and 595 nm and has a

transmission peak centred at 580 nm.

Use this filter with fluorochromes, such as TAMRA and

Cy3.

Transmits light between 595 nm and 625 nm and has a

transmission peak centred at 610 nm.

Use this filter with fluorochromes, such as ROX, EtBr,

SYPRO™ Red, and SYPRO Ruby.

Transmits light between 655 nm and 685 nm and has a

transmission peak centred at 670 nm.

Use this filter with fluorochromes, such as Cy5.

Transmits light below 526 nm.

Use this filter with fluorochromes, such as fluorescein

when scanning with the green laser.

Note: When using the blue laser, the 520 BP 40 might

provide better results.

Transmits light above 560 nm. Use this filter with

fluorochromes, such as TRITC.

This filter is used for storage phosphor scanning and is

not normally used for fluorescence scanning.

Typhoon Operating Instructions 28-9623-56 AA