Related Manuals for Thermo Scientific Heraeus Cryofuge 5500i
Summary of Contents for Thermo Scientific Heraeus Cryofuge 5500i
- Page 1 Thermo Scientific Heraeus Cryofuge 5500i Instruction Manual 20057781-e • 07 / 2020 Visit us online to register your warranty: thermofisher.com/labwarranty...
- Page 2 WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked by the following symbol:...
-
Page 3: Table Of Contents
Rotor Installation ..................... 4-3 Entering Parameters ....................4-4 Centrifugation ......................4-8 Temperature Adaptation during Standstill ............... 4-9 Short-term Centrifugation ..................4-9 Removing the Rotor ....................4-9 Aligning the Centrifuge ..................4-10 Audible Alarm ....................... 4-10 Key Switch ......................4-11 Thermo Scientific Cryofuge 5500i... - Page 4 Disinfection ......................5-3 Decontamination ..................... 5-4 Service of Thermo Fisher Scientific ................5-5 Chapter 6 Troubleshooting ........................6-1 Mechanical Emergency Door Release ..............6-2 Troubleshooting by User ..................6-3 When to contact a Service Technician ..............6-5 Cryofuge 5500i Thermo Scientific...
-
Page 5: Preface
CAUTION means that material damage could occur. WARNING means that injuries or material damage or contamination could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. Thermo Scientific Cryofuge 5500i... -
Page 6: Intended Use
Maximum sample density at maximum speed: 1.2 ---------- - Accident Prevention Prerequisite for the safe operation of the Heraeus Cryofuge 5500i is a work environment in compliance with standards, directives and trade association safety regulations and proper instruction of the user. - Page 7 • Rotor installation: Make sure the rotor is locked properly into place before operating the centrifuge. • Especially when working with corrosive samples (salt solutions, acids, bases), the accessory parts and vessel have to be cleaned carefully. • Always balance the samples. Thermo Scientific Cryofuge 5500i...
- Page 8 • Highly corrosive substances which can cause material damage and impair the mechanical stability of the rotor, should only be centrifuged in corresponding protective tubes. WARNING If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the area immediately. Cryofuge 5500i Thermo Scientific...
- Page 9 “Characteristics of the Cryofuge 5500i” page 1-2 “Technical Data” page 1-3 • “Directives, Standards and Guidelines” page 1-4 • “Functions and Features” page 1-4 • • “Mains Supply” page 1-5 “Refrigerants” page 1-6 • “Rotor Selection” page 1-6 • Thermo Scientific Cryofuge 5500i...
-
Page 10: Chapter 1 Introduction And Description
• The integrated rotor detection systems ensure that no inadmissible speed settings can be preselected. • Electronic imbalance detection, SMARTspin . • Door emergency release: For emergencies only, e.g. during power failures (see “Mechanical Emergency Door Release” page 6-2). Cryofuge 5500i Thermo Scientific... -
Page 11: Technical Data
-9 °C to +40 °C Dimensions Height 960 mm 960 mm 960 mm Width 680 mm 680 mm 680 mm Depth 720 mm 720 mm 720 mm Weight without Rotor 286 kg 286 kg 286 kg Thermo Scientific Cryofuge 5500i... -
Page 12: Directives, Standards And Guidelines
RCF-Selection, Temperature Regulation during Standstill, Pre-Temp, and PULSE Acceleration / Braking Profiles 9 Acceleration and 9 Braking Curves Rotor Recognition Automatic Imbalance Detection SMARTspin Electronic, contingent on Rotor and Speed Door Lock Motor assisted Door Looking Cryofuge 5500i Thermo Scientific... -
Page 13: Mains Supply
14.5 / 16 A 75004479 200 V 2.7 kW 16 AT 20 AT 60 Hz 14.5 A 75004479 208 V 2.7 kW 16 AT 20 AT 60 Hz 75004479 230 V 14.5 A 2.7 kW 16 AT 20 AT Thermo Scientific Cryofuge 5500i... -
Page 14: Refrigerants
1.43 t 75004479 Contains fluorinated greenhouse gases in a hermetically sealed system. Rotor Selection The Cryofuge 5500i is supplied without a rotor. Various Thermo Scientific rotors are available to choose from. LH-4000 75006475 with round Buckets 1000 75006477 Double rectangular Bucket DoubleSpin... -
Page 15: Before Use
“Setting up” page 2-2 “Aligning the Centrifuge” page 2-4 • “Transporting the Centrifuge” page 2-4 • “Location” page 2-4 • “Mains Connection” page 2-5 • “Adjust Voltage and Frequency” page 2-6 • “Storage” page 2-6 • Thermo Scientific Cryofuge 5500i... -
Page 16: Before Setting Up
1. Place the pallet with the centrifuge so that you have atleast 2 m room in front of the pallet. 2. Release the rails from the pallet. 3. Screw the rails onto the pallet in front of the centrifuge. Screws Rails Cryofuge 5500i Thermo Scientific... - Page 17 9. Screw the threaded nuts down. 10. Place the locking stabilizers centrally under the threaded rods and align the centrifuge. All the locking stabilizers should carry uniformly (check by a tilt test). The casters are some 5-15 mm off the ground. Thermo Scientific Cryofuge 5500i...
-
Page 18: Aligning The Centrifuge
People and hazardous substances must be kept out of the safety zone while centrifuging. • The supporting structure must be stable and free of resonance. • The supporting structure must be suitable for horizontal setup of the centrifuge. Cryofuge 5500i Thermo Scientific... -
Page 19: Mains Connection
3. Check whether the cable complies with the safety standards of your country. 4. Make sure that the voltage and frequency correspond to the figures on the rating plate. 5. Establish the connection to the power supply with the connecting cable. Thermo Scientific Cryofuge 5500i... -
Page 20: Adjust Voltage And Frequency
• Before storing the centrifuge and the accessories it must be cleaned and if necessary, disinfected and decontaminated. • Store the centrifuge in a clean, dust-free location. • Be sure to place the centrifuge on its feet. • Avoid direct sunlight. Cryofuge 5500i Thermo Scientific... -
Page 21: Control Panel
Control Panel Contents “Control Panel” page 3-2 • • “Keys” page 3-2 Thermo Scientific Cryofuge 5500i... -
Page 22: Keys
By pressing the key briefly, you increase or decrease the speed in one step. When pressing both arrows the cursor moves to the left. Cryofuge 5500i Thermo Scientific... -
Page 23: Operation
4-3 • • “Entering Parameters” page 4-4 “Centrifugation” page 4-8 • • “Temperature Adaptation during Standstill” page 4-9 “Short-term Centrifugation” page 4-9 • • “Removing the Rotor” page 4-9 “Aligning the Centrifuge” page 4-10 • Thermo Scientific Cryofuge 5500i... -
Page 24: Switch On Centrifuge
WARNING Do not reach into the crack between the door and the housing. The door is drawn shut automatically. Use the emergency release only for malfunctions and power failures (see “Mechanical Emergency Door Release” page 6-2). CAUTION Do not slam the door. Cryofuge 5500i Thermo Scientific... -
Page 25: Rotor Installation
6. If available close the rotor with the rotor lid. Be sure to check all sealings before starting any aerosol-tight applications. See the information in the rotor instruction manual. 7. Close the centrifuge door. Thermo Scientific Cryofuge 5500i... -
Page 26: Entering Parameters
10 rpm. The RCF can be set in steps of 1. 4. The value is stored when the value stops flashing. Note If an extremely low RCF value has been selected, it will be corrected automatically, if the resulting speed is less than 300 rpm. Cryofuge 5500i Thermo Scientific... - Page 27 Run Time Preselection 1. Press the key below the field running time. 2. Enter the desired value by pressing up or down. Up to 9 minutes 59 seconds the duration is given exactly to the second. Thermo Scientific Cryofuge 5500i...
- Page 28 1. Press the key below the field temperature. 2. Enter the desired value by pressing up or down. The temperature can be set in steps of 1. The value is stored when the value stops flashing. Cryofuge 5500i Thermo Scientific...
- Page 29 The value that is being changed flashes. 3. Press the key again when all displays are flashing again. The display with the number of the program memory flashes and all settings are stored. Thermo Scientific Cryofuge 5500i...
-
Page 30: Centrifugation
The display shows the following message: The run will terminate. Check the loading and start the centrifuge once again. See the information on proper loading in the rotor instruction manual. “Troubleshooting by User” page 6-3). For information on troubleshooting, see section Cryofuge 5500i Thermo Scientific... -
Page 31: Temperature Adaptation During Standstill
Note The centrifuge accelerates to maximum speed, regardless of which rotor was installed. Check carefully whether you have to maintain a certain speed for your application. During the acceleration process, time is counted forwards in seconds. The reading stays displayed until the centrifuge door is opened. Thermo Scientific Cryofuge 5500i... -
Page 32: Removing The Rotor
Note If the message rotor appears in the display, the selected speed or RCF value are too high. If you press the key the centrifuge accelerates to the shown maximum value. 4-10 Cryofuge 5500i Thermo Scientific... -
Page 33: Key Switch
All set parameters are blocked, but the actual program can be operated as often as needed with the control keys ( All set values can be seen with the arrow keys. The selected program cannot be changed. PULSE and the pre-temp function are locked. Thermo Scientific Cryofuge 5500i 4-11... - Page 35 Maintenance and Care Contents “Cleaning Intervals” page 5-2 • • “Cleaning” page 5-2 “Cleaning the Venting Slots” page 5-3 • • “Disinfection” page 5-3 “Decontamination” page 5-4 • • “Service of Thermo Fisher Scientific” page 5-5 Thermo Scientific Cryofuge 5500i...
-
Page 36: Chapter 5 Maintenance And Care
Clean centrifuge and accessories as follow: 1. Open the centrifuge. 2. Turn off the centrifuge. 3. Pull out the power supply plug. 4. Release the rotor. 5. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle. Cryofuge 5500i Thermo Scientific... -
Page 37: Cleaning The Venting Slots
Keep in mind the risk of infection when touching the rotor and take all necessary precautions. In case of contamination, make sure that others are not put at risk. Decontaminate the affected parts immediately. Take other precautions if need be. Thermo Scientific Cryofuge 5500i... -
Page 38: Decontamination
For general radioactive decontamination use a solution of equal parts of 70 % ethanol, 10 % SDS and water. 1. Open the centrifuge. 2. Turn off the centrifuge. 3. Pull out the power supply plug. Cryofuge 5500i Thermo Scientific... -
Page 39: Service Of Thermo Fisher Scientific
Thermo Fisher Scientific offers inspection and service contracts for this work. Any necessary repairs are performed for free during the warranty period and afterwards for a charge. This is only valid, if the centrifuge has only been maintained by a Thermo Fisher Scientific service technician. Thermo Scientific Cryofuge 5500i... -
Page 41: Troubleshooting
Troubleshooting Contents “Mechanical Emergency Door Release” page 6-2 • • “Troubleshooting by User” page 6-3 “When to contact a Service Technician” page 6-5 • Thermo Scientific Cryofuge 5500i... -
Page 42: Mechanical Emergency Door Release
4. Push the release cords back into the centrifuge and mount the plug. 5. Reconnect the centrifuge once the power has been restored. Switch on the centrifuge. Press the key to have the door locks operative again. Cryofuge 5500i Thermo Scientific... -
Page 43: Troubleshooting By User
After 15 sec. the rotor will be decelerated. Always wait until the rotor has come to a stop without braking. rotor By opening and closing the door you reset the message After entering a permissible speed, you can start again. Thermo Scientific Cryofuge 5500i... - Page 44 Restart the centrifuge. If an error message appears again, inform a service technician. E-10 Self-test after switching on the centrifuge. NV-RAM; error in program memory Restart the centrifuge. If an error message appears again, inform a service technician. Cryofuge 5500i Thermo Scientific...
-
Page 45: When To Contact A Service Technician
1. Turn on the power switch on the right side of the device. The centrifuge performs a self-check of its software. Subsequently, the following readings will be displayed. The order is: Software version key panel Software version NV-RAM-Version 1 Thermo Scientific Cryofuge 5500i... - Page 46 When to contact a Service Technician NV-RAM-Version 2 Cycle counter Note The values given are only examples. 2. Afterwards the different programs are tested as well. Program test 3. Communicate the software version to the service technician. Cryofuge 5500i Thermo Scientific...
- Page 47 U S S M S S U S S S S S S S S S U S S S S S S S S S S Boric Acid Cesium Acetate S S S - S S S S - S S - S S S S S S M S S S Thermo Scientific Cryofuge 5500i...
- Page 48 S M U S S S S M S U U U - Hydrofluoric Acid (50 %) U U U U - U U U U S U U U S S M M S U U U - Cryofuge 5500i Thermo Scientific...
- Page 49 S S S S - S S - S S S S S S M S S S Lactic Acid (100 %) M S U - S S S M S S - M S M S S - Thermo Scientific Cryofuge 5500i...
- Page 50 S S S S S S M S S S Rubidium Chloride Sucrose M S S - S S S S S S S S S S S S S S S S S S S S S S S Cryofuge 5500i Thermo Scientific...
- Page 51 S S S S S S S S S S S S S S S S S S S S S S S Citric Acid (10 %) M S S M S S M S S S S S S S S S M S S S S S S S S S S Polyethyleneterephthalate Thermo Scientific Cryofuge 5500i...
- Page 52 Performance unknown; suggest testing, using sample to avoid loss of valuable material. Chemical resistance data is included only as a guide to product use. No organized chemical resistance data exists for materials under the stress of centrifugation. When in doubt, we recommend pretesting sample lots. Cryofuge 5500i Thermo Scientific...
- Page 53 Explanation of RCF Value ......4-5 Run Time Preselection ........4-5 Features ............1-4 Safety features ..........1-2 Safety zone ............2-4 Select Program ..........4-7 Imbalance Indicator ........4-8 Service Technician ........5-5, 6-5 Short-term Centrifugation .......4-9 Starting Centrifuge Program ......4-8 Thermo Scientific Cryofuge 5500i...
- Page 54 Index Stopping the Centrifugation Program ..... 4-9 Storage ............2-6 Switch on Centrifuge ........4-2 Technical Data ..........1-3 Temperature Adaptation .........4-9 Transporting the Centrifuge ......2-4 Trouble Shooting ..........6-1 Cryofuge 5500i Thermo Scientific...
- Page 55 Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Germany thermofisher.com/centrifuge © 2000-2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise indicated. Heraeus is a registered trademark of Heraeus Holding GmbH licensed to Thermo Fisher Scientific. Delrin, TEFLON, and Viton are registered trademarks of DuPont.