Page 1 ® Operator’s Manual For Veterinary Use Only Customer and Technical Support: 1-800-822-2947 Available 24 hours a day, 7 days a week. March 2013 PN 1200-7063 Rev. A © 2013, Abaxis, Inc. Union City, CA 94587...
Page 2 Abaxis, VetScan, VetScan VS2, and iQC are registered trademarks of Abaxis. The information in this manual is relevant to analyzers P/N 1200-1001 and 1200-1001R. Analyzers 1200-1001 and 1200-1001R report to catalog numbers 1200-0000, 1200-0000E, 1200-0000R, and 1200-0000ER. U.S. Patent Nos. 5,061,381, 5,275,016,...
Page 3: Table Of Contents
Analyzer and Environmental Specifications....... . VetScan VS2 Chemistry Analyzer Overview.......
Page 4 Contacting Abaxis Technical Support ........
Page 5: Section 1: Quick Reference Guide
Quick Reference Section 1 Guide Analyzer Overview These figures show the VetScan VS2 Analyzer and its compo- nents. 1.1.1 Front and Back Views Printer and cover Touchscreen Rotor drawer Power button Handle Printer and cover USB ports DC power input...
Page 6: Installation Guidelines
Installation Guidelines 1.2.1 Step 1: Unpack Analyzer and Accessories Immediately upon receipt, place the box of reagent rotors in a refrigerator (2–8 ºC or 36–46 ºF). Remove the analyzer from its box and place it on a stable, level surface. Complete and mail the warranty card within 10 days of installing the system.
Page 7 See “Selecting Units” on page 5-16 for instructions on changing to SI units such as mmol/L. The VetScan VS2 is now ready to run samples. See “Test Procedure and Results” on page 4-1 instructions. Quick Reference Guide...
Page 8: Guidelines For Obtaining And Using Samples
Use only reagent rotors that have not expired, as noted on the pouch and box.  The VetScan VS2 Analyzer automatically rejects expired rotors. Do not remove the rotor from the refrigerator until you are ready to run the analysis.
Page 9 1.3.2 Step 2: Obtain a Sample Sample Types Whole blood samples: Use only lithium heparin test tubes (green top).  Plasma samples: Obtain these from whole blood samples (lithium heparin).  Serum: Obtain from a sample placed in a red top test tube. ...
Page 10 Test Tube Order To prevent contamination, always fill test tubes in this order: Blue top: Sodium citrate. Anticoagulant for coagulation only. Red top: No anticoagulant. Note: Use the red top test tube only if necessary. If the red top tube is not needed, fill the green top tube first.
Page 11 1.3.3 Step 3: Add Sample to the Rotor Sample Delivery Volume Follow these guidelines to make sure you add the correct sample volume to the reagent rotor. When a sufficient quantity of sample is added, it will  form a line between the arrows on the rotor surface. If an air bubble is introduced, add enough sample to ...
Page 12 Using a Disposable 100 µL Capillary Pipette Keep the capillary pipette horizontal until it is over the sample port. Raise the pipette to vertical (so it is perpendicular to the rotor sur- face), and place its tip in the sample port, as shown at right. Push the black plunger down slowly and smoothly until the white plug reaches the end of the capillary pipette.
Page 13: Section 2: General Information
CAUTION: If the VetScan VS2 Analyzer is used in any way other than described in this manual, the ana- lyzer may not operate as intended, may pro- duce inaccurate or no results, and may pose a safety hazard.
Page 14: Abaxis Technical Support
Abaxis Technical Support personnel can help with your questions about the VetScan VS2 Analyzer. Call Abaxis Technical Support, 24 hours a day, 7 days a week, at 1-800-822-2947. Symbols Used in Labeling The following symbols are found above the connectors or on the VetScan VS2 label, on the back of the analyzer. Symbol...
Page 15: Frequently Asked Questions
 Yes. The VS2’s extensive Intelligent Quality Control (iQC) detects any flaw or potential anomaly in the rotor. Abaxis also provides a Performance Guarantee covering manufacture- defective rotors. (This does not include cancellations or assay suppressions due to improper rotor storage, operator error, or inadequate sample integrity.) How many species can the VS2 store for reference ranges? ...
Page 16 Does the VS2 need surge protection or an uninterruptable power supply?  Abaxis recommends plugging the VS2 into a surge protector designed for use with a computer. Abaxis also recommends using an uninterruptable power supply (UPS) in areas prone to elec- trical surges or power outages.
Page 17 2.5.3 Samples What types of samples can I run on the VS2?  Whole blood, serum, or plasma. Is precision pipetting required?  No. Adding approximately 100–120 μ l of sample is sufficient. (Make sure not to overfill the chamber, or internal spills can result.) The VS2’s on-board microfluidics handle the rest. Can I use sodium heparin? ...
Page 18 Endogenous substances such as hemolysis, lipemia, and icterus affect all blood chemistries, regardless of the type, size, or cost of the analysis instrument. The VetScan VS2 is the only point-of-care analyzer that assesses and suppresses hemolysis, lipemia, and icterus by determining assay-by-assay the level at which these substances could affect the reliability of the results, and bringing this to the user’s attention.
Page 19 Can the VS2 results be transmitted to my lab management software?  Most lab management software is compatible with Abaxis equipment. Check with your soft- ware company to be certain. Can I back up the results on my VS2? ...
Page 20 General Information...
Page 21: Section 3: Set Up And Analyzer Description
Section 3 Description Unpacking the Analyzer Remove the VetScan VS2 Chemistry Analyzer from the shipping carton. Place the analyzer on a level sur- face relatively free of animal hair, dust, and other con- taminants. Do not place in a sunny window or near another heat source.
Page 22: Setting Up The Analyzer
Complete the warranty online at www.abaxis.com/warranty within 10 days of installation to start the warranty period. Customers are placed on the customer mailing list to receive infor- mation pertaining to the VS2 and ancillary products, such as software upgrades and new prod- uct announcements.
Page 23 Install a paper roll in the analyzer’s printer. a. Open the printer cover. If the printer contains a roll of paper, remove it. b. Remove the wrapper from the paper roll, then unroll several inches of printer paper. c. Put the paper roll into the printer so that the paper unrolls from the bottom of the roll and out towards the front of the analyzer, as shown.
Page 24 Customizing Reference Range feature described in “Customizing Reference Ranges” on page 5-2. Note: To order reagent rotors, pipettes, tips, and printer paper, contact an authorized distribu- tor, or contact Abaxis Customer Service at 1-800-822-2947 during regular business hours. Set Up and Analyzer Description...
Page 25: Analyzer And Environmental Specifications
Note: Be sure to become familiar with the VS2 before running samples. The VetScan VS2 Chemistry Analyzer consists of a portable analyzer and disposable single-use reagent rotors. Each reagent rotor contains all the reagents needed to perform a profile of tests on a single sample.
Page 26 The analyzer communicates to the operator through the display screen. The display gives procedural instruction, indicates the status of the analyzer, and presents error messages. The operator provides information to the analyzer through the touchscreen — for details, see “Touch- screen”...
Page 27: Reagent Rotors
The left and right arrow touchscreen keys move the display cursor forward or backward  to change a number on the display.   The right arrow key ( ) functions as a dash (–), and the left arrow key ( ) functions ...
Page 28 A Diluent Container is sealed with foil and can be seen in the center of the reagent rotor. The con- tainer is automatically opened after a rotor is inserted into the analyzer and the drawer is closed. The diluent is an aqueous solution which is used to dilute the plasma. Cuvettes are located along the perimeter of the reagent rotor and contain analyte-specific dry reagent beads.
Page 29: Section 4: Test Procedure And Results
Test Procedure and Section 4 Results Introduction to Testing Note: Use only VetScan Reagent Rotors with the VetScan VS2 Chemistry Analyzer. The basic testing procedure is listed below. The remainder of this section describes the details of collecting samples and preparing the reagent rotor (“Collecting Samples”...
Page 30: Collecting Samples
Touch on the touchscreen to open the ana- Analyze lyzer’s rotor drawer. Place the rotor in the drawer, then touch on the touchscreen. Close There is no need to orient the rotor in a particular direction. Analysis begins automatically when the drawer closes.
Page 31: Adding A Sample To The Rotor
Adding a Sample to the Rotor Remove the “practice” rotor from the start-up pack and practice loading a sample, using a preferred transfer device after reading the instructions in this section. CAUTION: The practice rotor is for sample loading practice only. DO NOT RUN THIS ROTOR IN THE VS2 —...
Page 32: Running Tests
Sample Delivery Volume Follow these guidelines to make sure you add the correct sample volume to the reagent rotor. When a sufficient quantity of sample is added, it will  form a line between the arrows on the rotor surface. If an air bubble is introduced, add enough sample to ...
Page 33 Turn on the analyzer power by pressing the Power button on the front of the analyzer. Do this before removing reagent rotors from the refrigerator. The analyzer starts up, then performs a self test. “Quality Control Fea- tures” on page 7-1 for infor- mation about the analyzer self test.
Page 34 Remove the reagent rotor from the pouch by holding the edges of the rotor. This eliminates the possibility of fingerprints on the optical surfaces of the rotor. CAUTION: Inspect the rotor for any damage before use. Use a transfer device to dispense ~100 μL of sample into the sample port. Note: Make sure whole blood samples are homogeneous before adding the sample to the reagent rotor.
Page 35 Patient identification numbers may be written in the space indicated at right. Do not write anywhere else on Write patient ID here the rotor or on the bar code ring. Hold the reagent rotor by its edges and keep it in a flat position while carrying it to the analyzer.
Page 36 Note: In the Load Rotor screen, touch the information icon to view additional help screens outlining the basic analysis procedure. (Touch to move through Next Back the screens, and to close them.) Exit 11. Place the rotor in the recessed area in the drawer. 12.
Page 37 14. Select the species of the sample being analyzed from the choices shown in the display. (The exact choices depend on how the analyzer is configured — the figure shows the default selections.) Note: The correct Type is necessary for results to be interpreted cor- rectly.
Page 38 17. Enter an ID number for the patient (up to 14 characters), then touch Done  The right arrow key ( ) functions as a dash (–), and the left  arrow key ( ) functions as a backspace. Enter an alternate ID (up to 14 characters), then touch Done CAUTION: Do not lean on the analyzer, nor place potentially hazardous material on the...
Page 39 20. When the sample is finished processing, the analyzer stores the results and shows that analysis is complete. 21. By default, the analyzer automatically prints the results of the analysis. If the results do not print automatically, they can be recalled from memory and printed — see “Searching Results,”...
Page 40: Canceling An Analysis
25. When finished, touch to close the drawer and return the ana- Close lyzer to standby mode. Canceling an Analysis To cancel an analysis in process, touch on the touchscreen. Cancel Touch to confirm. Cancel The analysis is then can- celed, and the rotor drawer opens.
Page 41: Using Test Results
Using Test Results The results calculated by the analyzer are stored in memory and printed automatically, and can be recalled and printed later as needed. If the analyzer is connected to an external computer, the results are automatically transmitted as soon as they are calculated. See “Principles of Operation”...
Page 42: Test Procedure Summary
“Troubleshooting” on page 8-1. Test Procedure Summary 4.7.1 VetScan VS2 Chemistry Analyzer Check that the electrical outlet utilized for the analyzer is grounded.  Check that the ambient temperature where the analyzer is located is 15–32 ºC ...
Page 43 4.7.2 Reagent Rotors Do not use an expired rotor. The reagent rotor expiration date is printed on the foil pouch  and is encoded in the bar code. Store all reagent rotors at 2–8 ºC (36–46 ºF) as described on their respective pouch ...
Page 44 4.7.4 Handling Samples Use the following guidelines and procedures to ensure best results. Syringe Guide Use the following sizes according to the size of the patient:  Small breed: up to 10 kg — use a 25-gauge syringe.  Medium breed: 10–30 kg — use a 22-gauge syringe. ...
Page 45: Section 5: Configuring The Analyzer
Configuring the Section 5 Analyzer This section describes how to configure the VetScan VS2 Chem- istry Analyzer to achieve excellent performance. Make general analyzer settings as shown in “Using the  Settings Screens” on page 5-2. Set species reference ranges for the specific patient popu- ...
Page 46: Using The Settings Screens
Using the Settings Screens The following shows the general path through the menus for performing the procedures in this section. These procedures are available whenever the analyzer displays the Home screen. In the Home screen, touch the Settings icon. The Analyzer Settings screen then opens. From here, adjust the date and time, language, sound, display, and printer, or view basic information about the ana- lyzer.
Page 47 Select the reference range to modify. To modify the last reference range that was changed, touch  Last , then skip to step page 5-4. Modified To modify any reference range, touch All, then continue to step 5.  The Modify Reference Ranges screen then opens. From here, the fol- lowing procedures are available: Modify the reference range for a specific analyte: see below.
Page 48  Select the reference range from the list. If needed, use the up (  and down ( ) arrow keys to view the entire list. Use the controls to set the upper and lower limits for the range.  ...
Page 49 The analyzer displays the ranges for the species.  Select the analyte to adjust. If needed, use the up ( ) and down  ) arrow keys to scroll through the list. Use the controls to set the upper and lower limits for the range. ...
Page 50 Touch to create a reference range for a new, unnamed Species 1 species, to create a range for a new control level, or Control 1 to create a reference range for a predefined species. Select To create a reference range for a new, unnamed species: ...
Page 51 To create a new control:  Touch Control 1 Set minimum and maximum values for each of the ana- lytes to be included in the range: i. Select the analyte to adjust from the list. If needed,   use the up ( ) and down ( ) arrow keys to view the entire list.
Page 52   Select the species. Use the up ( ) and down ( arrow keys to scroll through the list. Set minimum and maximum values for each of the ana- lytes to be included in the range.  i. Select the analyte to adjust. Use the up ( ) and ...
Page 53 5.2.4 Removing a Species Use this procedure to remove the reference ranges for a particular species. In the Modify Reference Ranges screen, touch Species Touch Remove Species   Select the species to remove. Use the up ( ) and down ( ) arrow keys to scroll through the list.
Page 54: Printing And Archiving Reference Ranges
Printing and Archiving Reference Ranges Reference ranges can be printed, or stored in the analyzer as archive files for later use or review. In the Home screen, touch the Settings icon. Touch the More Settings icon. Touch the Archive Reference Ranges icon. Touch Send To archive the reference ranges into the analyzer’s internal mem-...
Page 55: Retrieving Reference Ranges
To archive the ranges onto a PC connected to the analyzer:  Touch External Archive Select the species reference range to store. The reference range is then stored on the PC under the current date and time. To print a range: ...
Page 56: Transmitting Reference Ranges
A warning screen then appears. Touch to retrieve the Continue archived reference range. Transmitting Reference Ranges Reference ranges can be transmitted to a connected PC and stored for later use or review. In the Home screen, touch the Settings icon. Touch the More Settings icon.
Page 57: Viewing Analyzer Identification
Touch External Archive Select the species reference range to store. The reference range is then stored on the PC under the current date and time. Viewing Analyzer Identification Use this function to verify information about the analyzer, such as serial number and the version of the installed software.
Page 58: Changing Date And Time
Changing Date and Time The date and time is factory preset to Pacific Time. Reset the date and time as needed when setting up the analyzer, and periodically thereafter. In the Home screen, touch the Settings icon. Touch the Date and Time icon. Use the controls to set the time: ...
Page 59: Selecting The Language
Selecting the Language The analyzer provides several languages for menus and printing. Select the language to use (English is the default) as follows. In the Home screen, touch the Settings icon. Touch the Languages icon.  Select the language from the list. If needed, use the up ( ) and ...
Page 60: Selecting Units
Selecting Units You can select either Common Units, such as mg/dL, or SI Units (Systeme International), such as mmol/L, to report results. In the Home screen, touch the Settings icon. Touch the More Settings icon. Touch the Units Settings icon. Set the units used for all analytes, a particular analyte, or a class of analytes (electrolytes, enzymes, minerals, or proteins) as follows.
Page 61 To set the units for a particular analyte:  Touch Single Analyte Select the analyte from the list. If needed, use the up   ) and down ( ) arrow keys to view the entire list. Select the units to use for the analyte: g/dL mg/dL , or...
Page 62 To set units for all electrolytes:  Touch Analyte Groups Touch Electrolytes Select the units to use for all electrolytes: mmol/L mEq/L To set units for all enzymes:  Touch Enzymes 5-18 Configuring the Analyzer...
Page 63 Select the units to use for all enzymes: ukat/L To set units for all lipids:  Touch Lipids Select the units to use for all lipids: mg/dL mmol/L Configuring the Analyzer 5-19...
Page 64 To set units for all minerals:  Touch Minerals Select the units to use for all minerals: , or mg/dL mmol/L mEq/L To set units for all proteins:  Touch Proteins 5-20 Configuring the Analyzer...
Page 65: Setting Sound Volumes
Select the units to use for all proteins: g/dL 5.10 Setting Sound Volumes Use this procedure to adjust the volume of the sounds used for the analyzer’s screen click, alert, and status notification. In the Home screen, touch the Settings icon. Touch the Sound Settings icon.
Page 66: Adjusting The Display
5.11 Adjusting the Display You can adjust the brightness of the analyzer’s display as needed, and set the period that the analyzer must be inactive before it activates a screen saver or enters power-saving mode. (For either, touch the screen to wake the analyzer.) In the Home screen, touch the Settings icon.
Page 67 To adjust the screen saver delay:  Touch Screen Saver Use the controls to set the delay.   Use the up ( ) and down ( ) arrow keys to  adjust the delay. Touch to turn off the screen saver. ...
Page 68: Configuring Printers
Use the controls to set the delay.   Use the up ( ) and down ( ) arrow keys to  adjust the delay. Touch to prevent the analyzer from entering  Never power-saving mode. Touch to set the delay to its factory default. ...
Page 69 5.12.1 Setting the Default Printer Select the printer to use for printing results and reports as follows. In the Printer Setup screen, touch Configure Touch Set Default Select Analyzer Printer External Printer For External Printer: Select the printer from the displayed list of available printers. The analyzer then detects automatically detects the type of printer and configures it as needed.
Page 70 B-1. Note: Printer capabilities are subject to change without notice. For an up-to-date list of print- ers supported by the VetScan VS2, contact Abaxis Technical Support 24 hours a day, 7 days a week, at 1-800-822-2947. 5.12.4 Selecting Reports Use this procedure to set the type and number of reports that can be printed automatically after a sam- ple is analyzed.
Page 71: Setting Communication Protocol
Select the report type. Use the controls to adjust the number of copies to print.   Use the up ( ) and down ( ) arrow keys to adjust the num-  ber. Touch to set the number to zero. ...
Page 72: Setting Optional Advanced Functions
ASCII Text None ASTM Note: To use ASTM, contact Abaxis Technical Support 24 hours a day, 7 days week, at 1-800-822-2947. If you use veterinary management software, please contact your software supplier to ensure that your software is compatible with the VetScan VS2.
Page 73 In the Advanced Settings screen, select the types of information to be entered during the analysis process. For Alternate ID, select the particular alternate ID item to be entered.   Use the up ( ) and down ( ) arrow keys to scroll through the list.
Page 74 5-30 Configuring the Analyzer...
Page 75: Section 6: Recalling Results
Recalling Results Section 6 The VetScan VS2 Analyzer includes a Recall function that pro- vides access to the results of the most recent tests, including patient results, control results, and test errors. The Recall function is available from the analyzer’s Home screen.
Page 76: Recalling The Last Rotor Results
Recalling the Last Rotor Results Use this procedure to recall, print, or transmit the results of the last rotor run. In the Home screen, touch the Recall icon. Touch Last Rotor The results from the last rotor are displayed for review. You can use ...
Page 77: Searching Results
Searching Results Use the Recall function to search for a particular record according to its patient ID number or date, or to view a list of all patient or control results sorted by date. In the Home screen, touch the Recall icon. Touch Search Use the Search screen to search for results:...
Page 78 6.2.1 Searching for Results by Patient/Control ID In the Search screen, touch Patient/Control ID Enter the patient ID number.  The right arrow key ( ) functions as a dash (–), and the left arrow  key ( ) functions as a backspace. Touch Search The analyzer then displays a list of results (Patient, Control, and...
Page 79 To print these results or transmit them to an external computer: a. Touch Print b. Touch and/or Print Transmit c. Touch , or Results Error Report The display shows while the report or Sending Report... results are printing or transmitting. 6.2.2 Searching for Results by Date In the Search screen, touch...
Page 80 Touch to display the results.   Use the up ( ) and down ( ) arrow keys to scroll through the results. To print these results or transmit them to an external computer: a. Touch Print b. Touch and/or Print Transmit c.
Page 81: Browsing Results
Touch to display the results.   Use the up ( ) and down ( ) arrow keys to scroll through the results. To print these results or transmit them to an external computer: a. Touch Print b. Touch and/or Print Transmit c.
Page 82 The analyzer then displays a list of all results (Patient, Control, and Error) in reverse chronological order, beginning with the date entered.   Use the up ( ) and down ( ) arrow keys to scroll through the list and find the needed results. Touch to display the results.
Page 83: Transmitting Results
Transmitting Results Use this procedure to transmit all stored results to a computer. Note: The analyzer must be connected to a computer to transmit results — see “Connecting to an External Computer” on page 11-1. In the Home screen, touch the Recall icon. Touch Transmit All The analyzer displays “Transmitting All...”...
Page 84 6-10 Recalling Results...
Page 85: Section 7: Calibration And Quality Control
Section 7 Quality Control Calibration The VetScan VS2 Analyzer is self-calibrating. A bar code on the reagent rotor contains the information needed to calibrate the rotor when run. Additionally, each reagent bead used in the rotor is calibrated to a reference method and/or reference material.
Page 86 The analyzer’s software incorporates statistical methods to ensure accurate results. The lamp flashes up to 13 time points for rate chemistries, and typically three time points for endpoint chemistries. To ensure that the analyzer is functioning properly, the distribution of flashes at each time point is analyzed, and the standard deviation is compared to a pre-determined limit.
Page 87: Running Controls
c. Sample Samples are checked for physical interference. The analyzer estimates the sample indi- ces, hemolysis, lipemia, and icterus using absorbance readings for the sample at 340 nm, 405 nm, and 467 nm. This information is then compared to pre-established hemolysis, lipemia, and icterus limits for each method.
Page 88 In the Home screen, touch to open the rotor drawer. Analyze Insert the control sample into the reagent rotor (see “Running Tests” on page 4-4), and place the rotor into the drawer. Touch to close the drawer and start analysis. Close Enter the Operator ID using the touchscreen, then touch Done...
Page 89 Enter an alternate ID (up to 14 characters), then touch Done The analyzer checks the rotor type, then begins processing the sam- ple with no further input. The analyzer indicates when the sample is finished processing, then automatically prints the results of the analysis. If the results do not print automatically, they can be recalled from memory and printed —...
Page 90 Calibration and Quality Control...
Page 91: Section 8: Troubleshooting
Abaxis Technical Support, 24 hours a day, 7 days a week, at 1-800-822-2947, or send a fax to 1-877-900-9333.
Page 92 Contact Abaxis Technical Support at 1-800-822-2947 to review the patients results printed on the error report. You can print the error report using the Recall function starting from the Home screen. See “Recalling Results” on page 6-1. Disclaimer: Note that “~~~” is printed in the results for an individual chemistry when the instrument iQC detects a possible fault condition in the chemistry reading or cal- culation.
Page 93: Rotor Cancellations And Procedures
Rotor Cancellations and Procedures If the rotor cancels, record the following information or print an error report, and contact Abaxis Tech- nical Support at 1-800-822-2947: Lot number  Product name  4-digit error code  Specimen type and species ...
Page 94 Note: The next step is irreversible. All settings — including custom reference ranges — are returned to their factory values, and the memory is cleared. Results previously stored, however, are not affected. Touch to restore all factory settings. Continue Refer to the sections noted below to reset the date and time, any custom reference ranges, and the units of measurement: “Customizing Reference Ranges”...
Page 95: Section 9: Principles Of Procedure And Operation
The reagent rotor contains a diluent, and test-specific reagent beads. The operator then places the rotor in the VetScan VS2 Chemistry Analyzer and enters the appropriate identification numbers. The VS2 Analyzer automatically performs the remainder of the test- ing protocol.
Page 96: Principles Of Operation
Principles of Operation Chemical reactions occur between reagent beads, the diluent contained in the reagent rotor, and the added sample. These reactions produce chromophores that are measured photometrically by the VS2. The microprocessor then calculates the concentrations of the analytes. The measurement optics include a stroboscopic xenon lamp, a wavelength selection system, and a mul- tiple-wavelength detector.
Page 97 The equation for calculating absorbance is this: (I λ1RC - I λ1 ) / (I λ2RC - I λ2 A = -log (I λ1OC - I λ1 ) / (I λ2OC - I λ2 where: = Absorbance at wavelength 1, referenced to wavelength 2 = Intensity of light transmitted through the reaction cuvette at wavelength 1 λ1RC = Intensity of light transmitted through the reaction cuvette at wavelength 2...
Page 98 9.2.2 Endpoint Reactions Reagents, samples, and chromophores all absorb light in endpoint reactions. Light absorption by the samples must be subtracted so that the analyte concentration can be isolated. The absorbance of the sample is measured in a sample blank cuvette. Some chemistries employ a generic sample blank and others (such as total bilirubin) use a dedicated sample blank.
Page 99: Section 10: Maintenance And Service
Maintenance and Section 10 Service The VetScan VS2 Analyzer requires minimal maintenance. Clean the exterior of the analyzer weekly with mild detergent and a soft, damp cloth. The air filter requires cleaning once a month. Regular maintenance of the analyzer will assure reliable operation.
Page 100: Cleaning Spills
Check the filter frequently if the analyzer is in an environ- ment with excessive dust or animal hair. Note: For replacement filters, contact Abaxis Customer Service (1-800-822-2947, during regu- lar business hours). Turn the analyzer off by pressing and holding the Power button.
Page 101: Updating The Analyzer Software
10.4 Updating the Analyzer Software Abaxis provides software updates on CD-ROM to registered owners of the VetScan VS2 Analyzer. Replacement software is mailed immediately whenever a new software version is released. See “View- ing Analyzer Identification” on page 5-13 to determine the software version installed in the analyzer.
Page 102: Returning The Analyzer For Service
10.5 Returning the Analyzer for Service Before sending the analyzer in, you must obtain authorization for service: call Abaxis Technical Sup- port, 24 hours a day, 7 days a week, at 1-800-822-2947.
Page 103: Section 11: Connecting To An External Computer
Connecting to an Section 11 External Computer The VetScan VS2 Analyzer is equipped to transmit patient and control results in 8-bit, ASCII (American Standard Code for Information Interchange) format to a computer through a USB port. To receive the results from the VS2, the computer requires terminal emulation software, such as HyperTerminal, which is included with Windows 95 and higher.
Page 104: Transmission Specifications
L” and “mmo\L” is “mmol/L” in the transmitted results. 11.2 Enabling the VetScan VS2 Communication Protocol The VetScan VS2 can communicate with computers running Microsoft Windows, Macintosh OS X, or Linux (kernel 2.4 or above). To enable this communication, set the VS2’s communication protocol as follows: Follow steps 1 through 3 of “Setting Communication Protocol”...
Page 105: Installing The Windows Driver
“Installing the Windows Vista Driver” on page 11-7. Use this procedure to install the Abaxis driver that enables the analyzer to communicate with a PC run- ning the Windows operating system. The analyzer installs on the PC as a virtual communication port in Windows.
Page 106 Open the Win2K_XP folder. Double-click the file Abaxis Driver Setup.exe. Installation then begins. When the installer finishes, click Use the USB cable to connect the VS2 to any available USB port on the computer. Click , then click the tab.
Page 107 11. Note the port number to which the analyzer is assigned, and record this number. You will use this port number later in this procedure. (In the example at right, the port is COM4 12. Close the Device Manager. 13. Click , and in the dialog enter Start >...
Page 108 17. In the COM Properties dialog, use the pull- Bits per second down menu to select 9600 18. Transmit patient results from the VS2 patient archive. For instructions, see “Transmitting Results” on page 6-9. 19. Retransmit results from the VS2: a.
Page 109: Installing The Windows Vista Driver
11.4 Installing the Windows Vista Driver Use this procedure to install the Abaxis driver that enables the analyzer to communicate with a PC run- ning Windows Vista. The analyzer installs on the PC as a virtual communication port in Windows.
Page 110 Install the driver as follows. Insert the Abaxis Driver CD into the computer’s CD drive. The computer’s default web browser then opens, displaying the VS2 driver installation guide links. Close the browser. Go to to dis- Start > My Computer play the computer’s drives.
Page 111 Start > Control Panel > Hardware and Sound > Device Manager 10. Click the before Ports (COM & LPT). Look for Abaxis Blood Chemistry Analyzer. Note: If the analyzer does not appear in the device list, do not reinstall the driver. Unplug the USB cable from the computer, then reinsert it.
Page 112 b. Touch Last Rotor c. The results from the last rotor are displayed. Touch Print d. Select , and make sure is not selected. Xmit Print e. Touch Results The display shows while the results are Sending Report... transmitting. 11-10 Connecting to an External Computer...
Page 113: Installing The Mac Os X Driver
11.5 Installing the Mac OS X Driver Use this procedure to install the Abaxis driver that enables the Abaxis analyzer to communicate with a Mac. The analyzer installs on the Mac as a virtual communication port in Mac OS X.
Page 114 Click Restart Connect the USB cable to a Type A connector on the Mac and to the uppermost USB con- nector (Type B) on the back of the analyzer. Turn on the analyzer. Use the Network section in the System Pref- erences application ( Go >...
Page 115: Installing The Linux Driver
11.6 Installing the Linux Driver Use this procedure to install the Abaxis driver that enables the Abaxis analyzer to communicate with a PC running Linux. The analyzer installs on the PC as a virtual communication port. Installation requires the following: VetScan VS2 Analyzer ...
Page 116: Using Hyperterminal
Start > Settings > Control Panel > tab). Add/Remove Programs (Add/Remove Windows Components > Communications Consult Windows help for details. Type a descriptive name (such as Abaxis) for the con- nection in the Name box. Click 11-14 Connecting to an External Computer...
Page 117 In the Connect Using dialog, select the port that the ana- lyzer is connected to, then click . (The other settings are not required.) Note: If the port is unknown, select any one for now. If needed, you can run the wizard again to try other ports. Change Bits per second to , then click 9600...
Page 118 11.7.2 Capturing and Transmitting Results Prepare HyperTerminal to capture results by loading a saved session file or by creating a new session file as described in Section 11.7. Click on the menu. Select Transfer > Capture Text a path and file name and click Start Any results transmitted to the computer from this point forward will be captured in the designated...
Page 119: Section 12: Ordering Information
To order reagent rotors, pipettes, tips, printer paper, or other Vet- Scan supplies or accessories, contact your authorized distributor, or contact Abaxis Customer Service at 1-800-822-2947 during regular business hours. You can get the latest information online at this address: www.order.abaxis.com...
Page 120: Vetscan Reagent Rotors
12.3 VetScan Reagent Rotors Reagent Rotor Package Catalog Number Canine Wellness Profile Abaxis # 500-0044 Comprehensive Diagnostic Profile Abaxis # 500-0038 Prep Profile II Abaxis # 500-0026 Critical Care Plus Abaxis # 500-0042 /Cholesterol Profile Abaxis # 500-0037 Kidney Profile Plus...
Page 121: Section A: Iqc Codes
If the iQC code is not listed or if you cannot correct a particular problem, record the iQC number or print the report, and contact Abaxis Technical Sup- port, 24 hours a day, 7 days a week, at 1-800-822-2947, or send a fax to 1-877-900-9333.
Page 122 If the iQC code is not listed or if you cannot correct a particular problem, record the iQC number or print the report, and contact Abaxis Technical Support, 24 hours a day, 7 days a week, at 1-800-822-2947, or send a fax to 1-877-900-9333.
Page 123 If the iQC code is not listed or if you cannot correct a particular problem, record the iQC number or print the report, and contact Abaxis Technical Support, 24 hours a day, 7 days a week, at 1-800-822-2947, or send a fax to 1-877-900-9333.
Page 124 If the iQC code is not listed or if you cannot correct a particular problem, record the iQC number or print the report, and contact Abaxis Technical Support, 24 hours a day, 7 days a week, at 1-800-822-2947, or send a fax to 1-877-900-9333.
Page 125: Section B: Supported Printers
Supported Printers Appendix B Printers Supported by the VetScan VS2 The VetScan VS2 is compatible with most Hewlett-Packard (HP) PCL3-compatible USB printers. Abaxis has tested a limited number of these printers and validated their use with the VetScan VS2. The following VS2 printers are validated as of October 2012.
Page 126 Supported Printers...
Page 127: Section C: Methods Performance
Methods Appendix C Performance This appendix lists the materials required for operating the VetScan VS2, and the test panels provided by each profile rotor. Methods Performance...
Page 128 C.1 Materials Required The following materials are required to operate the VetScan VS2 according to the recommended procedure given in “Test Procedure Summary” on page 4-14. Materials Catalog Number Provided with Purchase of Reagent Rotor Profiles VetScan Reagent Profile “Ordering Information” on page 12-1.
Page 129 Methods Performance...
Page 130 C.3 Warnings and Precautions C.5 Indications of Reagent Rotor Instability or Deterioration • The diluent container in the reagent rotor is automati- cally opened when the analyzer drawer closes. A rotor • When stored as described above, all reagents con- with an opened diluent container cannot be re-used.
Page 131 C.8 Test Parameters sample collected from the patient. To accurately inter- The VetScan VS2 Chemistry Analyzer operates at ambi- pret glucose results, samples should be obtained from °...
Page 132 • Lithium heparin is the only anticoagulant recom- known values, or by using a serum-based, commercially mended for use with the VetScan VS2 Chemistry Ana- available control. See “Running Controls” on page 7-3.
Page 133 Ranges cited by Meyer, et al for dogs and cats were adjusted to reflect field data collected by Abaxis on the VetScan VS2 Chemistry Analyzer. Cutoffs for Bile Acids (BA) set for 100% specificity with a sensitivity of 74% for dogs and cats. See Ettinger SJ, Feldman EC, eds. St. Louis: W.B. Saunders Company.
Page 134 C.17 Performance Characteristics Linearity The chemistry for each analyte is linear over the dynamic range listed in Table 2 when the VetScan VS2 Analyzer is oper- ated according to the recommended procedure (see “Test Procedure and Results” on page 4-1).
Page 135 Table C-3: Correlation of VetScan System with Comparative Method Canine Feline Equine Bovine Analyte (n = 22–180) (n = 20–55) (n = 7–101) (n = 126) Alanine Aminotransferase (ALT) (U/L) Correlation (r) 1.00 0.98 0.97 Slope 0.95 0.92 0.94 Intercept Sample range 10–1549 27–99...
Page 136 Table C-3: Correlation of VetScan System with Comparative Method (Continued) Canine Feline Equine Bovine Analyte (n = 22–180) (n = 20–55) (n = 7–101) (n = 126) Creatinine (CRE) (mg/dL) Correlation (r) 0.99 1.00 0.95 Slope 1.00 1.01 1.00 Intercept -0.1 -0.4 Sample range...
Page 137: Section D: Species Labels
Species Labels Appendix D In addition to Dog, Cat, and Horse, the following species labels are available on the VS2 analyzer: Canines  Coyote Jackal   Dingo Wolf    Felines  Bobcat Lynx   Cheetah Panther ...
Page 138 Reptiles/Amphibians  Reptile Lizard Snake    Alligator Monitor Toad    Crocodile Python Tortoise    Frog Salamander Turtle    Small Mammals  Small Animal Hamster Rabbit    Hedgehog Raccoon   ...
Page 139 Index Controls, Running 7-3 Correlation C-9 Absorbance 9-3 Cuvettes 3-8 Advanced Functions 4-4 Setting 5-28 Alert Diluent Container 3-8 Volume 5-21, 5-22, 5-24 Direct Current Symbol 2-2 Bar Code Ring 3-8 Endpoint Reactions 9-4 Biohazard Symbol 2-2 Error Messages C-6 Expected Values C-7 Calibration 7-1, 9-1, C-6 Analyzer 7-1...
Page 140 Language Quality Control 7-1, C-6 Selecting 5-15 During Analysis 7-2 Limitations C-6 LIP 4-14, 6-1 Rate Methods 9-3 Lipemia 4-14 Reagent Rotors 3-7, 4-15, C-2 Lipemic Samples 4-14 Bar Code Ring 3-8 Cuvettes 3-8 Maintenance 10-1 Diluent Container 3-8 Air Filter 10-2 Expired 4-15 Exterior 10-1 Instability C-4...
Page 141 SI (Systeme International) 5-16 Caution 2-2 USB Connections 3-6 Direct Current 2-2 Network Port 2-2 USB Connection 2-2 VetScan VS2 Chemistry Analyzer Ambient Operating Temperature 3-5, C-5 Analyzer Identification 5-13 Test Panels C-2 Dimensions 3-5 Test Parameters C-5 Display 3-6...
Page 142: Index
Index...

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